Identification and Molecular Characterization of Bacteria Having Antimicrobial and Antibiofilm Activity

Sharma, Garima; Dang, Shweta; Gupta, Sanjay; Gabrani, Reema

doi:10.22159/ijpps.2016v8i10.12338

Cited by 11 publications

(12 citation statements)

References 23 publications

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“…The bacteria were cultured in nutrient broth at 37 ° C for 18-24 h. B. subtilis GAS101 was grown overnight in deMan, Rogosa and Sharpe (MRS) broth at 30 ° C for 24 h [11].…”

Section: Methodsmentioning

confidence: 99%

“…For the biofilm inhibition assay, a 1 × 10 6 CFU/mL sample of S. epidermidis was incubated with different concentrations of bacteriocin on a 96-well microtiter plate for 48 h at 37 ° C. The adhered cells of biofilm were stained with crystal violet (CV) dye as described by Sharma et al [11], and the absorbance of the plate was measured at 595 nm in an ELISA reader (BioRad, CA, USA).…”

Section: Antibiofilm Analysis Of Bacteriocinmentioning

confidence: 99%

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Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from Bacillus subtilis GAS101

et al. 2018

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Objective: The aim of this study was to purify and characterize bacteriocin from the soil isolate Bacillus subtilis GAS101, and to determine its antimicrobial as well as antibiofilm potential. The purified bacteriocin was further analyzed and evaluated for mammalian cell cytotoxicity and the possible mode of action. Material and Methods: Bacteriocin from B. subtilis GAS101 (an animal husbandry soil isolate) was partially purified and checked for antimicrobial and antibiofilm activity against gram-positive and gram-negative bacteria. The molecular weight of bacteriocin was determined using tricine SDS-PAGE gel. The stability of bacteriocin was investigated at various temperatures and pH levels, and its sensitivity towards 8 enzymes and 6 chemicals was determined. Cytotoxicity analysis was performed on a Vero cell line by a tetrazolium dye-based assay. Scanning electron microscopy (SEM) of bacteriocin-treated bacteria was carried out to determine the possible mode of action. Results: Bacteriocin from B. subtilis GAS101 was a potential inhibitor of both the indicator organisms (Staphylococcus epidermidis and Escherichia coli), and had a molecular weight of approximately 6.5 kDa. An in situ gel assay showed a zone of inhibition corresponding to the estimated protein band size. Bacteriocin was stable and showed antibacterial activity in broad ranges of temperature (30–121°C) and pH (2–12). It was sensitive to 4 proteolytic enzymes, which indicated its proteinaceous nature. Bacteriocin showed > 70% cell viability on the mammalian Vero cell line. SEM depicted that the bacteriocin was able to disrupt the bacterial cell membrane as its probable mode of action. Conclusion: Thermostable and pH-tolerant bacteriocin from B. subtilis GAS101, of about 6.5 kDa, showed broad-spectrum antimicrobial and antibiofilm activity.

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“…The bacteria were cultured in nutrient broth at 37 ° C for 18-24 h. B. subtilis GAS101 was grown overnight in deMan, Rogosa and Sharpe (MRS) broth at 30 ° C for 24 h [11].…”

Section: Methodsmentioning

confidence: 99%

Section: Antibiofilm Analysis Of Bacteriocinmentioning

confidence: 99%

Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from Bacillus subtilis GAS101

et al. 2018

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“…The extraction of whole genomic DNA from strain JAR8 was performed by following standard protocol described by Liu et al [11]. The amplification of 16S-rRNA gene of strain JAR8 was carried out using forward primer of approximately 400 g 5'-AGAGTTTGATCCTGGCTCAG-3ʹ and reverse primer of 400 ng 5'-GGTTACCTTGTTACGACTTC-3' [12]. The reaction mixture of 100 µl was prepared by mixing 2.5 mM each of dNTPs, 10X Taq polymerase assay buffer, and Taq DNA polymerase enzyme.…”

Section: Identification and Characterizationmentioning

confidence: 99%

Evaluation of Antimicrobial, Cytotoxicity, and Dyeing Properties of Prodigiosin Produced by Serratia Marcescens Strain Jar8

Chauhan

Choudhuri

Abraham

2017

Asian J Pharm Clin Res

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Objective: Prodigiosin is a red tripyrrole pigment which is synthesized as a secondary metabolite by biotypes A1, A2, and A6 of Serretia marcescens. This study was aimed to optimize production parameters for prodigiosin pigment produced by S. marcescens strain JAR8 and to determine its antimicrobial, cytotoxicity, and dyeing properties. Methods:The effect of various media components, process parameters (temperature, pH, incubation period), and other supplements was investigated, and the maximum production of prodigiosin was found to be at temperature 28°C, pH 7.0, incubation period of 5 day with peptone in the media. Antibacterial, cytotoxicity, and dyeing properties of prodigiosin were studied in depth. Results:The antimicrobial study of prodigiosin revealed that it is a potent inhibitor of Gram-positive bacteria as well as Gram-negative bacteria. The prodigiosin exhibited commendable dose-dependent cytotoxicity effect against human bone cancer cells with the IC50 of 108 µg/ml. Prodigiosin produced by strain JAR8 resisted the action of acid, alkali and detergent when applied to textile cloth. Conclusion:The deep red pigment prodigiosin produced by strain JAR8 is economically effective and can be categorized as microbial natural product for further pharmaceutical applications.

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“…Advancement in medical sciences has led to the increased use of invasive devices resulting in high rates of infection [1]. Listeria monocytogenes is a rod-shaped food-borne pathogen that is gram-positive, non-spore-forming, and strictly anaerobic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Charaterization of Listeria Monocytogenes Isolated From Different Sources in Iraq

Yousif

AL-Shamari

2018

Asian J Pharm Clin Res

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Objective: The main goal of the current study was to isolate and detect the phylogenetic characterization of Listeria monocytogenes isolate from clinical and non-clinical specimens.Methods: L. monocytogenes was isolated from 353 samples including: (94) Vaginal swabs, (81) piece of placenta, (51) frozen chicken, (69) soft cheese, and (58) frozen red meat samples using the Association of Official Agricultural Chemists method. Polymerase chain reaction (PCR) was performed for the detection of virulence gene hlyA followed by DNA sequence analysis for this gene.Results: A total of 13 isolates of L. monocytogenes were isolated from 2 (2.1%) vaginal swabs, 4 (4.9%) piece placenta, 2 (3.9%) frozen chicken, 3 (4.3%) frozen soft cheese, and 2 (3.4%) frozen red meat samples, and hlyA gene was detected in 100% of L. monocytogenes isolates.Conclusion: All the isolates tested positive for hlyA gene, so this is important role in the study of the L. monocytogenes infection and its pathogenicity. The phylogenetic analysis showed a clear convergence in the L. monocytogenes isolates from chicken, red meat, and soft cheese 70%, while there is a marked difference in isolates of L. monocytogenes isolated from placenta and vaginal swabs.

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Identification and Molecular Characterization of Bacteria Having Antimicrobial and Antibiofilm Activity

Cited by 11 publications

References 23 publications

Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from <b><i>Bacillus subtilis</i></b> GAS101

Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from <b><i>Bacillus subtilis</i></b> GAS101

Evaluation of Antimicrobial, Cytotoxicity, and Dyeing Properties of Prodigiosin Produced by Serratia Marcescens Strain Jar8

Phylogenetic Charaterization of Listeria Monocytogenes Isolated From Different Sources in Iraq

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