Evaluation of Antimicrobial, Cytotoxicity, and Dyeing Properties of Prodigiosin Produced by Serratia Marcescens Strain Jar8

Abstract

Objective: Prodigiosin is a red tripyrrole pigment which is synthesized as a secondary metabolite by biotypes A1, A2, and A6 of Serretia marcescens. This study was aimed to optimize production parameters for prodigiosin pigment produced by S. marcescens strain JAR8 and to determine its antimicrobial, cytotoxicity, and dyeing properties. Methods:The effect of various media components, process parameters (temperature, pH, incubation period), and other supplements was investigated, and the maximum production of prodigiosin was found to be at temperature 28°C, pH 7.0, incubation period of 5 day with peptone in the media. Antibacterial, cytotoxicity, and dyeing properties of prodigiosin were studied in depth. Results:The antimicrobial study of prodigiosin revealed that it is a potent inhibitor of Gram-positive bacteria as well as Gram-negative bacteria. The prodigiosin exhibited commendable dose-dependent cytotoxicity effect against human bone cancer cells with the IC50 of 108 µg/ml. Prodigiosin produced by strain JAR8 resisted the action of acid, alkali and detergent when applied to textile cloth. Conclusion:The deep red pigment prodigiosin produced by strain JAR8 is economically effective and can be categorized as microbial natural product for further pharmaceutical applications.

Prodigiosin/celecoxib-loaded into zein/sodium caseinate nanoparticles as a potential therapy for triple negative breast cancer

“…The mobile phase (10 mL) was put in a tight closed glass jar and left till saturation. Sample was then spotted on TLC plate and allowed to be developed 100 .…”

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“…Results of TLC developed with solvent system E showed 5 bands (Table 3) [7], system C [28] and system D [29] had shown no similar results: only one band with Rf varying between 0.10 and 0.14 depending to the strains (Table 3) for solvent system A, one large band (Rf: 0.58) for solvent system C and two bands (Rf: 0.56 and 0.75) for solvent system D (Not shown). This comparative analysis showed that the studied pigments, even their similarity with anthracyclines of prodigiosin family, exhibit different TLC profiles and should contain structural differences.…”

Yellow Pigment from Fast-growing Marine Soil Bacteria Citricoccus sp. for Dyeing Cotton Fabrics

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