Study and evaluation of a fluorimetric method for the determination of inorganic mercury in water

Mariscal, M. D.; Galbán, Javier; Urarte, Maria L.; Aznárez, José A.

doi:10.1007/bf00321713

Cited by 3 publications

(4 citation statements)

References 29 publications

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“…According to the reported literature, a notorious number of fluorometric methods are based on the quenching effect of Hg(II) on the fluorescence of a reagent, such as rhodamine B, 28 bathophenanthrolinedisulfonate, 24 meso-tetrakis(4-trimethylammoniumphenyl)porphyrin 26 or binaphthyl-based amphiphiles. 9 Mercury methods based on increasing fluorescence intensity measurements are based on one of the following approaches: the effect of the Hg(II) on the oxidation of a reagent to yield a fluorescent derivative, 6,13 the formation of ion-association complex with a fluorescent reagent and extraction to an organic phase, 2,15,16 and the selective formation of a fluorescent complex of mercury. Of course, the latter approach is the more selective one, but methods based on the formation of fluorescent complexes of the metal are scarce.…”

Section: Analytical Characteristicsmentioning

confidence: 99%

“…In addition, mercury methods based on increasing fluorescence intensity measurements are scarce, of course, which is the more selective one. 2,4,6,7,10,12,15,16 Previously, several hydrazones, of which salicylaldehyde and 2-hydroxy-1-naphthaldehydene were parent substances, were synthesized in our laboratory. They were used as spectrofluorometric reagents for the determination of aluminum, 17,18 strontium, 19 copper, 20 molybdenum, 21 lead 22 and chromium.…”

Section: Introductionmentioning

confidence: 99%

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Highly Sensitive Spectrofluorometric Determination of Trace Amounts of Mercury with a New Fluorescent Reagent, 2-Hydroxy-1-naphthaldehydene-8-aminoquinoline

Jiang

2006

ANAL. SCI.

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The principalship of chemical analysis, especially in environmental sciences, is the determination and quantification of harmful substances. The determination of mercury in this type of sample is one of the problems, but, as already acknowledged, is a very important one. Firstly, there is the inherent toxicity of the element at low concentrations (water with a content of over 1 µg L -1 is considered to be contaminated). Secondly, there is the ease with which mercury is biologically transformed into alkyl-mercury compounds, which are longer lasting and more dangerous toxins, 1,2 which can result in death or severe damage to the brain. 3 In recent years, due to its involvement in poisoning incidents, its use in industry has somewhat declined. Despite this, it is still widely used, and is the most ubiquitous of all heavy metals, because it is the only metal that can exist as a liquid and as a volatile form at ambient temperature. It is thus necessary to monitor the mercury levels in products for human consumption and environmental samples.Molecular fluorescence is attracting a great deal of interest in environmental monitoring, since it is inherently more sensitive than spectrophotometry, 31 and thus it can be used for the analysis of very low trace concentrations. As far as mercury is concerned, only a few methods have been described so far for its fluorometric determination. [5][6][7][8][9][10][11][12][13][14] Moreover, the majority of these methods present problems of sensitivity, selectivity, reaction time or procedure complexity. In addition, mercury methods based on increasing fluorescence intensity measurements are scarce, of course, which is the more selective one. 2,4,6,7,10,12,15,16 Previously, several hydrazones, of which salicylaldehyde and 2-hydroxy-1-naphthaldehydene were parent substances, were synthesized in our laboratory. They were used as spectrofluorometric reagents for the determination of aluminum, 17,18 strontium, 19 copper, 20 molybdenum, 21 lead 22 and chromium. 23 In order to increase the degree of molecular conjugation and the coplanar effect of a particular complex, it is necessary to design the molecular structure. In the present work, a new fluorescent reagent, 2-hydroxy-1-naphthaldehydene-8-aminoquinoline (HNAAQ), was synthesized, and its complex with mercury was studied. The fluorescent intensity of the complex was obviously increased by a coplanar effect, and the degree of molecular conjugation, owing to the presence of a naphthalene ring and a quinoline ring at both ends of the reagent's structure. Moreover, according to the Lewis "Hard and Soft Acids and Bases Rules", Hg 2+ is a soft acid and -N=CH-and the pyridine ring are borderline bases. It is therefore very possible and easy that Hg 2+ and N of "-N=CH-" and N of the pyridine ring can be complexed with each other. They formed a steady structure, probably as follows:Based on this complexation, a new spectrofluorometric method with high sensitivity was developed for the determination of mercury. After a concentration step for th...

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Section: Analytical Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Spectrofluorometric Determination of Trace Amounts of Mercury with a New Fluorescent Reagent, 2-Hydroxy-1-naphthaldehydene-8-aminoquinoline

Jiang

2006

ANAL. SCI.

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“…However, the amount of total mercury in rivers contaminated with industrial, mining, and urban waste occasionally reaches mid-ppt to low-ppb levels (30). An important application of rapid mercury assays could be to monitor whether the mercury concentrations in environmental water samples complies with the regulatory limit, 1 ppb in many countries (30,31). The sensitivities of some of our assays appear to be adequate for this use and are similar to those of the cold-vapor atomic absorption spectrometry and a current immunoassay method (32).…”

Section: Resultsmentioning

confidence: 99%

Development of Rapid Mercury Assays. Synthesis of Sulfur- and Mercury-Containing Conjugates

1995

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We have devised rapid analyses for mercury exploiting the high affinity of dithiocarbamate chelators for the mercuric ion. Our first assay is based on a sandwich chelate formed by a ligand supported on the well of an ELISA plate, Hg2+ ion of the investigated sample, and another ligand bound to a reporter enzyme. The second assay utilizes competitive binding of the analyte Hg2+ ions versus an organomercury conjugate to a chelating conjugate. Low ppb sensitivity and high selectivity for Hg2+ ions have been achieved in our pilot studies.

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“…The detection limit was 0.15 pg Hg per liter. Finally, Hg(I1) was determined after solvent extraction (n-butyl acetate) and fluorescence excitation at 452 nm and detection at 476 nm [159].…”

Section: Mercurymentioning

confidence: 99%

Advances in Flow Analysis – Instrumentation and its Application in Environmental Analysis

Berman¹

1995

Quality Assurance in Environmental Monitoring

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Study and evaluation of a fluorimetric method for the determination of inorganic mercury in water

Cited by 3 publications

References 29 publications

Highly Sensitive Spectrofluorometric Determination of Trace Amounts of Mercury with a New Fluorescent Reagent, 2-Hydroxy-1-naphthaldehydene-8-aminoquinoline

Highly Sensitive Spectrofluorometric Determination of Trace Amounts of Mercury with a New Fluorescent Reagent, 2-Hydroxy-1-naphthaldehydene-8-aminoquinoline

Development of Rapid Mercury Assays. Synthesis of Sulfur- and Mercury-Containing Conjugates

Advances in Flow Analysis – Instrumentation and its Application in Environmental Analysis

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