Development of Rapid Mercury Assays. Synthesis of Sulfur- and Mercury-Containing Conjugates

Szurdoki, Ferenc; Kido, Horacio; Hammock, Bruce D.

doi:10.1021/bc00032a001

Cited by 13 publications

(4 citation statements)

References 34 publications

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“…Fluorescence generation strategies could provide a unique template for fingerprinting of various cells or biological components in the presence of a specific antibody for drug- and immuno-targeting purposes . The strategy for immuno-targeting is shown as Scheme .…”

Section: Introductionmentioning

confidence: 99%

“…32 Fluorescence generation strategies could provide a unique template for fingerprinting of various cells or biological components in the presence of a specific antibody for drugand immuno-targeting purposes. 33 The strategy for immunotargeting is shown as Scheme 1. In this study, we also examined the effect of metal ion coordination on fluorescence properties of the chitosan-gelatin conjugates.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

2005

Biomacromolecules

153

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Polysaccharide-protein conjugations have generated increasing interests for biomedical applications in recent years. A naturally occurring cross-linking reagent, genipin, which has been used in herbal medicine, was employed to cross-link chitosan and gelatin for the preparation of a novel chitosan-gelatin conjugate. The primary amine groups on chitosan and gelatin were covalently linked with genipin, leading to the formation of a chitosan-gelatin conjugate with nitrogen-containing heterocycle units, the pyrindine-like derivatives. The FT-IR and UV-vis studies revealed that chitosan could react with genipin via a nucleophilic ring-opening reaction to construct more sufficient and extensive cross-link networks, as compared with its gelatin counterpart. The UV-vis absorption properties of the chitosan-gelatin conjugates were strongly related to the chitosan-to-gelatin weight ratio in the compositions. It is worth noting that the conjugation process endows the special emission properties of the chitosan-gelatin conjugates, which depends on the cross-linking reaction and the formation of hydrogen bonding involved chitosan-gelatin complex. Fluorescence quenching or enhancement was observed from the chitosan-gelatin conjugates upon coordinated with a wide variety of heavy metal ions (Ag+, Cu2+, Fe2+, and Co2+). This study also examined the possibility of covalent coupling the capture chelator (chitosan) with bioactive protein (e.g., albumin, alpha-globulin, and fibrinogen) to create fluorescence emission. These findings may provide a novel way to deliver therapeutic radionuclides for immuno-targeting purposes in the future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

2005

Biomacromolecules

153

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“…New rapid assays for mercury, based on the formation of sulfur containing chelates have been devised (Szurdoki et al, 1995a(Szurdoki et al, , 1995b. One of these assays involves a sandwich chelate formed by a chelator supported on the well of the ELISA plate, the mercuric ¡on, and a chelator linked to the reporter enzyme ( Figure 1).…”

Section: Other Toxic Chemicals Metabolitesmentioning

confidence: 99%

Rapid assays for environmental and biological monitoring

Szurdoki¹,

Jaeger²,

Harris³

et al. 1996

Journal of Environmental Science and Health, Part B

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Rapid, inexpensive, sensitive, and selective enzyme-linked immunosorbent assays (ELISAs) now are utilized in environmental science. In this laboratory, many ELISAs have been developed for pesticides and other toxic substances and also for their metabolites. Compounds for which ELISAs have recently been devised include insecticides (organophosphates, carbaryl, pyrethroids, and fenoxycarb), herbicides (s-triazines, arylureas, triclopyr, and bromacil), fungicides (myclobutanil), TCDD, and metabolites of naphthalene and toluene. New rapid assays have been developed for mercury.

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“…It is necessary to carry out continuous control of pesticide residues in food. Immunological methods using monoclonal or polyclonal antibodies are one of these alternatives (Szurdoki et al 1995, Li et al 2009Limei et al 2008). There are many ELISA for determination of low concentrations of pesticides.…”

Section: Introductionmentioning

confidence: 99%

Immunofluorescence assay of pesticides on the base of immobilized multi-polyclonal antibody

Ivanov

Yaneva

Godjevargova

et al. 2019

FSAB

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The sensitive competitive immunofluorescence method for simultaneous determination of paraoxon and dichlorvos with immobilized multi-polyclonal antibody on magnetic nanoparticles was developed. The multi-polyclonal antibody was obtained after the immunization of mixture of two prepared immunogens dichlorvos-cBSA and paraoxon-BSA (1:1). The immunogens dichlorvos-cBSA and paraoxon-BSA were synthesized preliminary. Multi-polyclonal antibody against dichlorvos and paraoxon was covalently coupled on magnetic nanoparticles. The competitive fluorescence conjugates dichlorvos-cBSA-FITC and paraoxon-BSA-ATTO 620 were synthesized. Two typical calibration curves of immunofluorescence assay for determination of dichlorvos and paraoxon in buffer solutions were obtained. The linear interval from 2 to 200 ng/mL for these two pesticides was determined. Then the calibration curves for dichlorvos and paraoxon were obtained in cow milk solutions. The linear range of pesticides in cow milk was determined (from 5 to 300 ng/L) and the detection limit for paraoxon (3.5 ng/mL) and dichlorvos (4 ng/mL) was found. The obtained results for cow milk samples were compare with results in UHT, pasteurized cow milk, sheep and goal milk. Quite different are the results when analyzing paraoxon and dichlorvos in standard solutions prepared in sheep's milk. The linear working range for the two pesticides is between 7 and 300 ng/mL it is obviously, that there is a shift of all analytical characteristics up to higher values. The reason for this is the high fat content of sheep's milk. The obtained results were showed that the developed method was 2 time more sensitive than method with the results obtained with mixture (1:1) of two separated antibodies – anti-paraoxon and anti-dichlorvos. These results confirmed the potential of the immunoassay for quantitative simultaneous screening of both dichlorvos and paraoxon. Practical applications:The use of immunofluorescence assay based on immobilized multi-polyclonal antibody on magnetic nanoparticles allows us to detect simultaneous paraoxon and dichlorovos in raw milk and ather diary products.

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Development of Rapid Mercury Assays. Synthesis of Sulfur- and Mercury-Containing Conjugates

Cited by 13 publications

References 34 publications

Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

Rapid assays for environmental and biological monitoring

Immunofluorescence assay of pesticides on the base of immobilized multi-polyclonal antibody

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