Study and application of flow injection spectrofluorimetry with a fluorescent probe of 2-(2-pyridil)-benzothiazoline for superoxide anion radicals

Tang, Bo; Zhang, Li; Zhang, Lili

doi:10.1016/j.ab.2003.11.023

Cited by 35 publications

(15 citation statements)

References 17 publications

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“…From product analysis and the fluorescence properties, we propose that the mode of reaction of O 2 – · with DBZTC is as follows (Scheme 2). Upon treatment with O 2 – ·, DBZTC, an almost nonfluorescent compound, was oxidized at pH 7.40 by deleting hydrogen [19] to yield DBZTC oxide, which has better rigidity and a larger conjugated system (free electron pairs on S also conjugating with phenyl ring).…”

Section: Resultsmentioning

confidence: 99%

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Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe

Gao

Tang

et al. 2007

The FEBS Journal

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Quantitation of superoxide radical (O 2–·) production at the site of radical generation remains challenging. A simple method to detect nanomolar to micromolar levels of superoxide radical in aqueous solution has been developed and optimized. This method is based on the efficient trapping of O2–· using a novel fluorescent probe (2‐chloro‐1,3‐dibenzothiazolinecyclohexene), coupled with a spectra character‐signaling increase event. A high‐specificity and high‐sensitivity fluorescent probe was synthesized in‐house and used to image O2–· in living cells. Better selectivity for O2–· over competing cellular reactive oxygen species and some biological compounds illustrates the advantages of our method. Under optimal conditions, the linear calibration range for superoxide anion radicals was 5.03 × 10−9−3.33 × 10−6 m. The detection limit was 1.68 × 10−9 m. Fluorescence images of probe‐stained macrophages stimulated with 4β‐phorbol 12‐myristate 13‐acetate were obtained successfully using a confocal laser scanning microscope.

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Section: Resultsmentioning

confidence: 99%

“…Buffer concentration also affects fluorescence intensity. We showed that the relative fluorescence intensity of the system was high and stable at buffer concentrations of [16][17][18][19][20][21][22] (v ⁄ v %) (Fig. 3B).…”

Section: Effect Of Ph and Buffer Concentrationmentioning

confidence: 99%

Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe

Gao

Tang

et al. 2007

The FEBS Journal

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“…[40,83] Die Sonden wurden speziell im Hochdurchsatz-Screening auf SOD-Aktivitäte ingesetzt, um O 2 C À in RAW264.7-Zellen, Zebrabärblingen und Mausmodellen fürEntzündungen und Diabetes sichtbar zu machen. [84]…”

Section: Benzothiazolinunclassified

Materialien mit Selektivität für oxidative Molekülspezies für die Diagnostik und Therapie

et al. 2020

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Abbildung 1. A) Krankheiten, fürdie oxidativer Stress bedeutsam ist. B) Primäre Untergruppen oxidativer Molekülspezies, ihre Entstehungsund Transformationsreaktionen und ihre Halbwertszeit. a Die Halbwertszeit der verwandten Radikale hängt von den Umgebungsbedingungen ab. Caiyan Zhao ist als Postdoktorandin Stipendiatin an der Havard Medical School. Sie wurde 2018 in physikalischer Chemie am National Center for Nanoscience and Technology,C hinese Academy of Science (China) promoviert. Dann wechselte sie zur Fuzhou University und ist dort seit 2018 Postdoktorandin. In ihren Forschungenkonzentriert sie sich auf die Entwicklung antioxidativer und multifunktionaler Nanoplattformen zur Freisetzung von Therapeutika von niedermolekularen Substanzen bis hin zu RNA fürdie Behandlung entzündlicher Erkrankungen. Jinjun Shi ist Associate Professora nder Harvard MedicalS chool und Fakultätsmitglied am Center for Nanomedicine and Department of Anesthesiology am Brigham and Women'sHospital. Er befasst sich mit Grundlagen-und angewandter Forschung im Nanomedizinbereich. Dabei geht es um eine interdisziplinäre Kombination aus Nanotechnologie, Biomaterialien, RNA-Freisetzung, Immuntherapie und antioxidativer Therapie fürb iomedizinische Anwendungen gegen Verletzungen und verschiedeneK rankheiten wie Krebs. Er wurde 2008 an der Texas A&MUniversity promoviertund arbeitete als PostdoktorandamMIT und am Brigham and Woman'sHospital. Jibin Song promovierte 2014 in Chemical and BiomedicalE ngineeringa nder Nanyang Technological University (Singapur). Danach arbeitete er als Postdoktorand bei Prof. Xiaoyuan (Shawn) Chen an den National Institutes of Health (NIH). Er wechselte 2018 zur Fuzhou University als "Min Jiang Scholar"-Professor füranalytische Chemie. Seine Forschung dreht sich vor allem um die Entwicklungv on Nanosonden fürm olekulare Bildgebung fürB ioimaging, Biosensoriku nd Wirkstoff-oder Genfreisetzung.

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“…Superoxide-induced oxidation can be discriminated from other cellular oxidative processes by monitoring fluorescent emissions. Tang et al [21] described a sensitive probe, 2-(2-pyridyl)benzothiazoline, that could be used to determine O 2…”

Section: Introductionmentioning

confidence: 99%

“…•-led to a strongly fluorescent product [21]. Medvedeva et al [22] synthesized a pyrene nitroxide based compound to monitor superoxide radical production in a xanthine/xanthine oxidase system.…”

Section: Introductionmentioning

confidence: 99%

A novel functional imidazole fluorescent ionic liquid: simple and efficient fluorescent probes for superoxide anion radicals

et al. 2013

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Novel imidazole fluorescent ionic liquids with anthracene groups (ImS-FILA) were synthesized for the first time to act as fluorescent probes. They were developed for the determination of superoxide anion radicals (O2 (•-)) in an aqueous system. O2 (•-) was produced by pyrogallol autoxidation. The fluorescence of ImS-FILA was quenched by superoxide anion radicals. The π-bond structure of the fluorescent molecules was oxidized and damaged. This method is very simple and sensitive. The linear range of sensitivity was 1-70 μM ImS-FILA, and the detection limit for reactive oxygen species was 0.1 μM. This method was used to detect superoxide radicals in papaya and garlic, with satisfactory results. Further work is needed to demonstrate the utility of this method in detecting reactive oxygen species in a biological aqueous system.

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Study and application of flow injection spectrofluorimetry with a fluorescent probe of 2-(2-pyridil)-benzothiazoline for superoxide anion radicals

Cited by 35 publications

References 17 publications

Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe

Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe

Materialien mit Selektivität für oxidative Molekülspezies für die Diagnostik und Therapie

A novel functional imidazole fluorescent ionic liquid: simple and efficient fluorescent probes for superoxide anion radicals

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