Materialien mit Selektivität für oxidative Molekülspezies für die Diagnostik und Therapie

Abstract

Abbildung 1. A) Krankheiten, fürdie oxidativer Stress bedeutsam ist. B) Primäre Untergruppen oxidativer Molekülspezies, ihre Entstehungsund Transformationsreaktionen und ihre Halbwertszeit. a Die Halbwertszeit der verwandten Radikale hängt von den Umgebungsbedingungen ab. Caiyan Zhao ist als Postdoktorandin Stipendiatin an der Havard Medical School. Sie wurde 2018 in physikalischer Chemie am National Center for Nanoscience and Technology,C hinese Academy of Science (China) promoviert. Dann wechselte sie zur Fuzhou University und ist dort seit 2018 Postdoktorandin. In ihren Forschungenkonzentriert sie sich auf die Entwicklung antioxidativer und multifunktionaler Nanoplattformen zur Freisetzung von Therapeutika von niedermolekularen Substanzen bis hin zu RNA fürdie Behandlung entzündlicher Erkrankungen. Jinjun Shi ist Associate Professora nder Harvard MedicalS chool und Fakultätsmitglied am Center for Nanomedicine and Department of Anesthesiology am Brigham and Women'sHospital. Er befasst sich mit Grundlagen-und angewandter Forschung im Nanomedizinbereich. Dabei geht es um eine interdisziplinäre Kombination aus Nanotechnologie, Biomaterialien, RNA-Freisetzung, Immuntherapie und antioxidativer Therapie fürb iomedizinische Anwendungen gegen Verletzungen und verschiedeneK rankheiten wie Krebs. Er wurde 2008 an der Texas A&MUniversity promoviertund arbeitete als PostdoktorandamMIT und am Brigham and Woman'sHospital. Jibin Song promovierte 2014 in Chemical and BiomedicalE ngineeringa nder Nanyang Technological University (Singapur). Danach arbeitete er als Postdoktorand bei Prof. Xiaoyuan (Shawn) Chen an den National Institutes of Health (NIH). Er wechselte 2018 zur Fuzhou University als "Min Jiang Scholar"-Professor füranalytische Chemie. Seine Forschung dreht sich vor allem um die Entwicklungv on Nanosonden fürm olekulare Bildgebung fürB ioimaging, Biosensoriku nd Wirkstoff-oder Genfreisetzung.

Dye-Triplet-Sensitized Downshifting Nanoprobes with Ratiometric Dual-NIR-IIb Emission for Accurate In Vivo Detection

“…Due to the short half-lives, high reactivity, and short diffusion distance of ClO – and other ROS, the quantitative detection of ClO – is still challenging by other established methods . Nevertheless, we believed that our in vivo ratiometric detection could potentially approach real data quantification based on in vitro investigation.…”

“…Plotting ratiometric signals (F 808ex /F 980ex ) against ClO – concentration (0 to 16 μM) resulted in a strong linear correlation ( R 2 = 0.998, Figure e). Notably, the limit of detection (LOD) of this system was calculated to be 77 nM, which is much lower than the ClO – concentration in the physiological environment. , To further confirm the reliability of IR-808@DSNPs@DSPE-PEG-NH 2 for ratiometric imaging (detection) of ClO – , we examined these ratiometric signals under 1% intralipid with 1 mm depths and found that they could remain consistent without being affected by fluorescence attenuation (Figure S26). This result was in accordance with the result of Figure c, verifying the effectivity of IR-808@DSNPs@DSPE-PEG-NH 2 for ratiometric detection of ClO – in vivo .…”

“…As an endogenous reactive oxygen species (ROS), ClO – is generated from the reaction between hydrogen peroxide and chloride ions in the presence of myeloperoxidase (MPO) and is regarded as a biomarker in biological tissue for indicating pathologies, such as inflammation. − Real-time detection of ClO – levels can be used for monitoring inflammation development and providing assistance in diagnosis. Previous reports have documented that heptamethine cyanine dyes can be easily involved in oxidation reactions and IR-808 was always specifically oxidized by ClO – , , which also confirmed by MS spectra of the IR-808 with and without ClO – (Figure S25).…”

“…Due to the short half-lives, high reactivity, and short diffusion distance of ClO − and other ROS, the quantitative detection of ClO − is still challenging by other established methods. 62 Nevertheless, we believed that our in vivo ratiometric detection could potentially approach real data quantification based on in vitro investigation. To verify this, we intend to use the NIR-IIb ratiometric imaging with a fixed ClO − concentration for identifying the consistency of the concentration of ClO − in vivo by our established method and the real concentration of ClO − in the inflammation location (Figure S31a).…”