Studies Using Anterior Pituitary Hormones as Antigens.

Studies on Human Growth Hormone. I. A Radio-Immunoassay for Human Growth Hormone*

“…The antigenic specificity of HGH has been studied and extensively described by previous workers (7,9,24,25). Our observations with radio-immunoprecipitation confirm the information obtained with more orthodox immunologic procedures showing that antisera induced in rabbits by HGH reacts only with other primate growth hormones and not with growth hormones of other species.…”

Immunogenicity of Synthetic Angiotensin II Octapeptide and Analysis of Antibodies by Passive Haemagglutination

“…Haemagglutination inhibition has found wide application, not only for the determination of peptide hormones in biological material, but also as a means for the immunological analysis of antibodies [1,2,22,23,36,50,57,61,62]. In the present study, an attempt was made to analyze by haemagglutination inhibition some of the structural features determining immunological specificity of anti-angiotensin produced by immunization of rabbits with either All-carrier complexes or with All adsorbed on to bentonite.…”

Antibody Production Induced by Hormonal Therapy

“…First, Arquilla & Stavitsky (1956a) showed that the serum of insulin-treated rabbits and of some human diabetics would agglutinate sheep red cells coated with insulin. Since then agglutination of red cells coated with the homologous hormone has been demonstrated with serum of rabbits treated with corticotrophin (Fishman et al 1959), somatotrophin (Read & Stone 1958), mammotrophin (Levy & Sampliner 1961), chorionic gonadotrophin (Wide & Gemzell 1960), and thyrotrophin (Cline et al 1960). In each case agglutination is inhibited by prior addition of the homologous hormone to the serum and is not produced by normal serum.…”