Studies to explore the UVA photosensitizing action of 9-phenylacridine in cells by interaction with DNA

Hansda, Surajit; Mitra, Anindita; Ghosh, Rita

doi:10.1080/15257770.2021.1880011

Cited by 4 publications

(2 citation statements)

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“…Compounds exhibiting noncovalent interactions with DNA generally display hypochromism owing to the stacking interactions between the aromatic chromophore and the DNA base pairs. The extent of binding was understood from the intrinsic binding constant ( K b ) values deduced from the Wolfe–Shimmer equation . The plots of [DNA]/(ε a – ε f ) versus [DNA] are shown in Figure S61, and the K b values are listed in Table S6.…”

Section: Resultsmentioning

confidence: 99%

“…The extent of binding was understood from the intrinsic binding constant (K b ) values deduced from the Wolfe−Shimmer equation. 36 The plots of [DNA]/(ε a − ε f ) versus [DNA] are shown in Figure S61, and the K b values are listed in Table S6. The complexes holding PPh 3 (7−12) displayed better binding than complexes 1−6.…”

Section: Importantly Trans-[imi-h]-[rucl 4 (Imi) 2 ] and Trans-[ind-h...mentioning

confidence: 99%

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Ru(II)-p-Cymene Complexes of Furoylthiourea Ligands for Anticancer Applications against Breast Cancer Cells

et al. 2023

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Half-sandwich Ru(II) complexes containing nitro-substituted furoylthiourea ligands, bearing the general formula [(η 6 -p-cymene)RuCl 2 (L)] (1−6) and [(η 6 -p-cymene)RuCl(L)(PPh 3 )] + (7−-12), have been synthesized and characterized. In contrast to the spectroscopic data which revealed monodentate coordination of the ligands to the Ru(II) ion via a "S" atom, single crystal X-ray structures revealed an unusual bidentate N, S coordination with the metal center forming a four-membered ring. Interaction studies by absorption, emission, and viscosity measurements revealed intercalation of the Ru(II) complexes with calf thymus (CT) DNA. The complexes showed good interactions with bovine serum albumin (BSA) as well. Further, their cytotoxicity was explored exclusively against breast cancer cells, namely, MCF-7, T47-D, and MDA-MB-231, wherein all of the complexes were found to display more pronounced activity than their ligand counterparts. Complexes 7−12 bearing triphenylphosphine displayed significant cytotoxicity, among which complex 12 showed IC 50 values of 0.6 ± 0.9, 0.1 ± 0.8, and 0.1 ± 0.2 μM against MCF-7, T47-D, and MDA-MB-231 cell lines, respectively. The most active complexes were tested for their mode of cell death through staining assays, which confirmed apoptosis. The upregulation of apoptotic inducing and downregulation of apoptotic suppressing proteins as inferred from the western blot analysis also corroborated the apoptotic mode of cell death. The active complexes effectively generated reactive oxygen species (ROS) in MDA-MB-231 cells as analyzed from the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) staining. Finally, in vivo studies of the highly active complexes (6 and 12) were performed on the mice model. Histological analyses revealed that treatment with these complexes at high doses of up to 8 mg/kg did not induce any visible damage to the tested organs.

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Section: Resultsmentioning

confidence: 99%

Section: Importantly Trans-[imi-h]-[rucl 4 (Imi) 2 ] and Trans-[ind-h...mentioning

confidence: 99%