Studies Regarding the Establishment of a Viable Micropropagation and Acclimatization Protocol for Brassica Oleracea L. Plants Obtained From in Vitro Culture

In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

“…Hybrid seed production is very difficult for Brassica species. Obtaining the commercial hybrids of Brassica oleracea L. is a highly laborious effort because they are strictly cross-pollinated plants and the maintaining of parental lines used in hybrid combinations involves a lot of time and financial support (Balkaya et al, 2005;Cristea et al, 2009;Karaagac and Kar, 2016;Dogru et al, 2017). The low price of seeds has limited the opportunities for mass micropropagation of vegetables and thereby restricted the use of clonal multiplication for breeding purposes (Kieffer et al, 2001).…”

“…This technology allows the regeneration of a large number of plants, independently of the season, in controlled conditions. The genetic material thus obtained is authentic, homogeneous, healthy, high quality, and productive, avoiding phytosanitary risks and early biological cycles (Cristea et al, 2009). However, the development of in vitro regeneration techniques is useful to produce uniform and true-to-type plants of the cultivar and is also essential for the future recovery of transgenic plants (Ravanfar et al, 2014;Kurtar et al, 2020).…”

“…For genetic improvement of cabbage using genetic engineering, efficient and reproducible in vitro regeneration protocols are required. In Brassica, remarkable results regarding the regeneration of new plants using different explants such as cotyledon and hypocotyl were obtained through direct organogenesis and embryogenesis (Cristea et al, 2009;Gambhir et al, 2017). Brassica vegetable improvement programs started in the 1990s in Türkiye.…”