<i>In vitro</i>maintenance of CMS lines of Indian cauliflower: an alternative for conventional CMS-based hybrid seed production

Bhatia, Reeta; Dey, S. S.; Sharma, Kanika; Parkash, Chander; Kumar, Raj

doi:10.1080/14620316.2015.11513169

Cited by 11 publications

(6 citation statements)

References 11 publications

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“…The IBA used in the media had a positive effect on the average root diameter in all cabbage genotypes. These findings are similar to the results obtained from in vitro propagation studies carried out on Brassica species (Munshi et al, 2007;Bhatia et al, 2015).…”

Section: Average Root Diametersupporting

confidence: 90%

“…The auxin group plant growth regulators used in white cabbage in the media do not affect the rooting rate but have positive effects on healthy and strong root development (Gerszberg et al, 2015). On the other hand, root formation was weaker in cauliflower plants without auxin in the rooting media (Bhatia et al, 2015). x Aaccording to the Duncan multiple comparison test, the difference between the means indicated by the same letter is not statistically significant.…”

Section: Number Of Branching Rootsmentioning

confidence: 99%

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In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

Dogru¹,

Balkaya

Kurtar

2022

Black Sea Journal of Agriculture

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Brassica species are the most widely cultivated vegetable crops and improvement programs started in the last thirty years in Türkiye. Hybrid seed production is very difficult for Brassica vegetable species. Because the production of a new F1 hybrid cultivar needs a male sterile line (A), maintainer line (B), and also a male line (C). Biotechnological methods provide an excellent opportunity for new F1 hybrid cultivar improvement via in vitro maintenance of the breeding lines. Thus, in vitro propagation possibilities of Matsunami F1 cultivar and 3 white head cabbage maintainer inbred lines were examined using hypocotyl and cotyledon explants. The most successful results were obtained from MS + 2 mg/l and 4 mg/l BAP combinations. Matsunami F1 was prominent in terms of proliferation rate. Root formation was found to be considerably high in all genotypes. The use of in vitro propagation techniques is expected to provide significant benefits in head cabbage breeding programs.

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Section: Average Root Diametersupporting

confidence: 90%

Section: Number Of Branching Rootsmentioning

confidence: 99%

In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

Dogru¹,

Balkaya

Kurtar

2022

Black Sea Journal of Agriculture

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“…The application of CMS provides an alternative to using self-incompatible lines during crop breeding (Wang et al, 2012 ). Currently, CMS is widely used for hybrid breeding and seed production of important vegetable crops, including onion (Havey, 2000 ), cabbage (Fang et al, 2001 , 2004 ), radish (Lee et al, 2008 ), and cauliflower (Dey et al, 2011 ; Kaminski et al, 2012 ; Bhatia et al, 2015 ). Similarly, CMS lines play a crucial role in broccoli breeding and hybrid production (Mao et al, 2012 ; Shu et al, 2014 ; Jing et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers

Shu

Liu

et al. 2016

Front. Plant Sci.

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Broccoli (Brassica oleracea var. italica) is an important commercial vegetable crop. As part of an efficient pollination system, cytoplasmic male sterility (CMS) has been widely used for broccoli hybrid production. Identifying the original sources of CMS in broccoli accessions has become an important part of broccoli breeding. In this study, the diversity of the CMS sources of 39 broccoli accessions, including 19 CMS lines and 20 hybrids, were analyzed using mitochondrial markers. All CMS accessions contained the ogu orf138-related DNA fragment and the key genes of nap CMS, pol CMS, and tour CMS were not detected. The 39 CMS accessions were divided into five groups using six orf138-related and two simple sequence repeat markers. We observed that ogu CMS R3 constituted 79.49% of the CMS sources. CMS6 and CMS26 were differentiated from the other accessions using a specific primer. CMS32 was distinguished from the other accessions based on a 78-nucleotide deletion at the same locus as the orf138-related sequence. When the coefficient was about 0.90, five CMS accessions (13CMS6, 13CMS23, 13CMS24, 13CMS37, and 13CMS39) exhibiting abnormal floral organs with poor seed setting were grouped together. The polymerase chain reaction amplification profiles for these five accessions differed from those of the other accessions. We identified eight useful molecular markers that can be used to detect CMS types during broccoli breeding. Our data also provide important information relevant to future studies on the possible origins and molecular mechanisms of CMS in broccoli.

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“…Our group has developed large number of DH lines on Brassica vegetables through isolated microspore culture [41, 52, 53]. The improved CMS lines of cauliflower have also been developed indigenously through protoplast fusion followed by recurrent backcrossing [9, 54]. Till date, only limited information is available regarding combining ability, gene action and heterosis using CMS systems and DH lines to improve yield and quality traits in B .…”

Section: Introductionmentioning

confidence: 99%

Heterosis and combining ability in cytoplasmic male sterile and doubled haploid based Brassica oleracea progenies and prediction of heterosis using microsatellites

et al. 2019

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In Brassica oleracea , heterosis is the most efficient tool providing impetus to hybrid vegetable industry. In this context, we presented the first report on identifying superior heterotic crosses for yield and commercial traits in cauliflower involving cytoplasmic male sterile (CMS) and doubled haploid (DH) lines as parents. We studied the suitability of genomic-SSRs and EST-SSRs based genetic distance (GD) and agronomic trait based phenotypic distance (PD) for predicting heterosis in F 1 hybrids using CMS and DH based parents. 120 F 1 hybrids derived from 20 Ogura based CMS lines and 6 DH based testers were evaluated for 16 agronomic traits along with the 26 parental lines and 4 commercial standard checks. The genomic-SSRs and EST-SSRs based genetic structure analysis grouped the 26 parental lines into 4 distinct clusters. The CMS lines Ogu118-6A, Ogu33A, Ogu34-1A were good general combiner for developing early maturity hybrids. The SCA effects were significantly associated with heterosis suggesting non-additive gene effects for the heterotic response of hybrids. Less than unity value of σ 2 A/D coupled with σ 2 gca /σ 2 sca indicated the predominance of non-additive gene action in the expression of studied traits. The correlation analysis of genetic distance with heterosis for commercial traits suggested that microsatellites based genetic distance estimates can be helpful in heterosis prediction to some extent.

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In vitromaintenance of CMS lines of Indian cauliflower: an alternative for conventional CMS-based hybrid seed production

Cited by 11 publications

References 11 publications

In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers

Heterosis and combining ability in cytoplasmic male sterile and doubled haploid based Brassica oleracea progenies and prediction of heterosis using microsatellites

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