Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers

Shu, Jinshuai; Liu, Yumei; Li, Zhansheng; Zhang, Lili; Fang, Zhiyuan; Yang, Limei; Zhuang, Mu; Zhang, Yangyong; Lv, Honghao

doi:10.3389/fpls.2016.00927

Cited by 26 publications

(28 citation statements)

References 39 publications

(40 reference statements)

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“…A wellcharacterized male sterile cytoplasm which prevents pollen development is available in Petunia (Gillman et al, 2009) and can be transferred by backcross to elite female lines in a breeding program. In this context, molecular markers associated to cytoplasm types are of value to trace sterile cytoplasms during their introgression into elite lines and to determine genetic purity of F1s (Shu et al, 2016;Swamy et al, 2017;Ferreira and Santos, 2018).…”

Section: Discussionmentioning

confidence: 99%

PCR identification of Petunia male sterile cytoplasm

Colombo

Hagiwara²

2019

Ornam. Hortic.

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Petunia is a very important ornamental plant with a broad range of flower colour and size, and most of the cultivars grown are propagated through seeds. Cytoplasmic male sterility (CMS) is a maternally inherited character determined by mitochondrial genes that results in impaired pollen development. The unique and well characterized male sterile cytoplasm in Petunia is a valuable resource for hybrids production because it prevents self-fertilization of mother plants and ensures the purity of F1s. Introgression of the male sterile cytoplasm in elite lines of Petunia is achieved following a backcross scheme and can be assisted using molecular markers associated to the trait of interest. The objective of this study was to develop a molecular marker to identify the male sterile cytoplasm of Petunia. A PCR-based marker amplifying a region of the mitochondrial CMS-associated urfS only in the male sterile plants was designed. Results showed differential PCR amplification of a ≈ 600 bp product in plants carrying male sterile cytoplasm in four Petunia species and their F1s and BC1 generations. A multiplex PCR reaction was subsequently set up, adding specific primers amplifying a ≈ 800 bp product from the conserved region trnT-trn-L of the chloroplast genome as a positive control in order to unambiguously identify the cytoplasm types as normal or sterile. A rapid, simple and precise molecular marker is now available for assisting breeding of F1 hybrids in Petunia.

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Section: Discussionmentioning

confidence: 99%

PCR identification of Petunia male sterile cytoplasm

Colombo

Hagiwara²

2019

Ornam. Hortic.

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“…On the other hand, hybrid cultivars from male sterility lines have enhanced seed purity and hybrid rates, and DGMS lines usually have higher seed yields than Ogura-CMS line from the same inbred line [11,13]. The Ogura-CMS lines of broccoli and other Brassica plants used today have been improved by protoplast fusion [11], and Ogura-CMS is stable and easily transferred between species; therefore, these lines are important sources of CMS in Brassica plants [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed genes and phenotypic variation in florets among the isogenic Ogura-CMS, DGMS and inbred lines of broccoli

Li¹,

Liu²,

Fang³

et al. 2020

Preprint

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Background: Male sterility contributes strongly to hybrid seed purity and production in Brassica crops. To detect plant phenotypes and the gene expression patterns involved in bioprocess of an Ogura cytoplasmic male sterility line (Ogura-CMS), a dominant genic male sterility line (DGMS) and their maintainer line, we analyzed the transcriptomes of broccoli florets among the isogenic Ogura-CMS (T54C), DGMS (T54M) and inbred lines (T54S).Results: There were respectively 505, 585 and 469 upregulated genes, and 1109, 1073 and 543 downregulated genes in the comparison groups of T54C and T54M, T54C and T54S, and T54M and T54S. The head weight and head width showed stronger performance in the Ogura-CMS than the DGMS line or maintainer line. The Ogura-CMS line showed poorer performance in seed yield and seed germination than the DGMS line or maintainer line. The DGMS line had longer maturation and flowering periods than the Ogura-CMS and maintainer lines. The plant hormone genes, auxin (TIR1, GH3 and SAUR) and salicylic acid (SA) (NPR1), and adenosine triphosphate-binding cassette (ABC) transporters-related genes (ABCB1 and ATM) were highly up-regulated in T54C compared with T54M or T54S. The brassinosteroid upregulated gene CYCD3 related to the function of late-flowering and delaying senescence in plants, was detected in T54M over two seasons.Conclusions: Among the isogenic Ogura-CMS, DGMS and inbred lines of broccoli, the Ogura-CMS line showed strong performance in head yield than the DGMS line or the inbred line. The DGMS line had longer days to flowering than the Ogura-CMS and inbred lines. However, the inbred line presented a higher seed yield and seed germination rate than the DGMS or Ogura-CMS line. This study found that some potential plant hormone genes, auxin (TIR1, GH3 and SAUR) and SA (NPR1), and ABC transporters related genes (ABCB1 and ATM) might play a key role in regulation of the developmental trait in the Ogura-CMS line. The BR- upregulated gene CYCD3 may function in late-flowering and delaying senescence of broccoli consistent with the investigations conducted over two seasons. Our findings provided a possible explanation for physiological and developmental differences of broccoli among the Ogura-CMS, the DGMS and the inbred lines.

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“…Therefore, presently cytoplasm induced male sterility are most widely used in hybrid breeding in cauliflower [13,19]. The wild allies and other closely related Brassica coenospecies serve as the reservoir of different cytoplasms whichare being used to develop CMS throughintraspecific/interspecific/intergeneric hybridization mediated by sexual crossing or protoplast fusion [11,[20][21][22][23][24]. Among the different CMS types, ogu [25]and pol [26]CMS systems are widely used in hybrid breeding of vegetableBrassicas.Once developed the CMS phenotype and the associated mitochondrial genes exhibit a maternal inheritance pattern.Numerous CMS related genes (orfs) have been identified and well characterized in different plant species.…”

Section: Introductionmentioning

confidence: 99%

“…oleracea var. italica [23,37], B. oleracea var. capitata [38], B. rapa [39][40][41], B. napus [39] and B. juncea [42].…”

Section: Introductionmentioning

confidence: 99%

“…or PISTILLATA, which are class B genes of classic ABC model for flower development, renders homeotic transformation of stamens to carpels or petals to sepals [37,[46][47] [6,[22][23][24]49].In India, during the last three decades, a large number of CMS based breeding lines/material have been generated in Indian cauliflowers by repeated back crossing or somatic hybridization by exploiting various CMS sources.…”

Section: Introductionmentioning

confidence: 99%

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Elucidating mitochondrial DNA-markers of Ogura-based CMS linesin Indian cauliflowers (Brassica oleracea var. botrytis L.) and their floral abnormalities due to diversity in cyto-nuclear interaction

Singh

Bhatia

Kumar

et al. 2020

Preprint

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Background: Mitochondrial markers can be used to differentiate diverse mitotypes as well as cytoplasms in angiosperms. In cauliflower, cultivation of hybrids is pivotal in remunerative agriculture and cytoplasmic male sterile lines constitute an important component of the hybrid breeding. Thus, the breeders look for utilizing diverse male sterile cytoplasms in hybrid progamme. In diversifying source of male sterility,it is essential to appropriately differentiate among the available male sterile cytoplasms in cauliflower.PCR polymorphism atthe key mitochondrial genes associated with male sterility will be useful in developing mitochondria specific markers for the different male sterile cytoplasms. Also, the auto and alloplasmic cytonuclear combinations result in complex floral abnormalities.Thus, the study aimed at developing mitotype specific markers of the sterile cytoplasms and to unravel thegenetic effects of the cytonuclear interactions on flower morphology in Indian cauliflowers.Results: In PCR based analysis using a set of primers targeted to orf-138, 76 Indian cauliflower lines showed presence of Ogura cytoplasm though amplicons showed polymorphism within the ofr-138 sequence. The polymorphic loci were found to be spanning over 200-280 bp and 410-470bp genomic regions of BnTR4 and orf125, respectively. Sequence analysis revealed that such cytoplasmic genetic variations could be due to single nucleotide polymorphisms and insertion or deletions of31/51 nucleotides.The cytoplasmic effects on varying nuclear-genetic backgrounds led to varying degree of floral malformations ranging from reduction in flower size, stamens and style length, modification in position of styleand anthers, absence of non-functional stamens to other floral abnormalities. These floral malformations caused dysplasia of flower structure affecting female fertility and inefficient nectar production.Conclusions: The mitochondria specific markers can distinguish ogurabased male sterile cytoplasm. Large number of Indian cauliflower lines showed mitotype variations even within the ogura based cytoplasm. Variable nucleo-cytoplasmic interactions resulted into diverse type of floral malformationsin addition to pollen sterility even within the group of ogura based CMS lines. The finding provide important reference ameliorate understanding of mechanism of cytonuclear interactions in floral organ development in Brassicas. The study will help the breeders in selecting CMS lines without any floral abnormalities in B. oleracea.

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Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers

Cited by 26 publications

References 39 publications

PCR identification of Petunia male sterile cytoplasm

PCR identification of Petunia male sterile cytoplasm

Differentially expressed genes and phenotypic variation in florets among the isogenic Ogura-CMS, DGMS and inbred lines of broccoli

Elucidating mitochondrial DNA-markers of Ogura-based CMS linesin Indian cauliflowers (Brassica oleracea var. botrytis L.) and their floral abnormalities due to diversity in cyto-nuclear interaction

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