Studies on wound healing potential of polyherbal formulation using in vitro and in vivo assays

Talekar, Yogesh P.; Apte, Kishori; Paygude, Shubhangi V.; Tondare, Prasad R.; Parab, Pradeep B.

doi:10.1016/j.jaim.2016.11.007

Cited by 62 publications

(68 citation statements)

References 21 publications

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“…The migration of ovarian cancer cells is critical for their tumorigenic properties and examined by the experiment of cell migration assay [36]. The present results showed the average healing rate of OVCAR-3 and HO-8910 was significantly greater than that of HOSEpiC ( Fig.2a and b), which is consistent with the changes of viscoelastic results by AFM, indicating the relationship between migratory potential and viscoelastic properties of ovarian cancer cells.…”

Section: Tumorigenic Properties Of Ovarian Cancer Cellssupporting

confidence: 86%

Association Examined of Viscoelastic Properties with the Invasion of Ovarian Cancer Cells by Atomic Force Microscopy

Chen¹,

Zeng²,

Ruan³

et al. 2019

Preprint

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Cellular mechanical properties could serve as a prominent indicator for disease progression and early cancer diagnosis. This study utilized atomic force microscopy (AFM) to measure the viscoelastic properties and then examined their association with the invasion of ovarian cancer at living single cell level. The results demonstrated the elasticity and viscosity of ovarian cancer cell OVCAR-3 and HO-8910 significantly decreased than those of HOSEpiC, the ovarian cancer control cell. Further examination found the dramatic increase of migration/invasion and the obvious decease of microfilament density in OVCAR-3 and HO-8910 cells compared with those of HOSEpiC cells. And there was a significant relationship between viscoelastic and biological properties among these cells. In addition, the elasticity was significantly increased in OVCAR-3 and HO-8910 cells after the treatment of anticancer compound echinomycin (Ech), while no obvious change was found in HOSEpiC cells after Ech treatment. Interestingly, Ech seemed no effects on the viscosity of these cells. Furthermore, Ech significantly inhibited the migration/invasion and significantly increased the microfilament density in OVCAR-3 and HO-8910 cells compared with those of HOSEpiC cells, which was significantly related with the elasticity among these cells. Notably, an increase of elasticity and a decrease of invasion were found in OVCAR-3 and HO-8910 cells with Ech treatment. Together, this study clearly demonstrated the association of viscoelastic properties with the invasion of ovarian cancer cells and shed a light on the biomechanical changes for early diagnosis of tumor transformation and progression at single cell level.

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Section: Tumorigenic Properties Of Ovarian Cancer Cellssupporting

confidence: 86%

Association Examined of Viscoelastic Properties with the Invasion of Ovarian Cancer Cells by Atomic Force Microscopy

Chen¹,

Zeng²,

Ruan³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In order to determine the relationship between viscoelastic and tumorigenic properties of ovarian cancer cells, the present study further examined the migration and invasion changes of these cells besides the microfilament density of F-actin cytoskeleton. The migration of cancer cells is critical for their tumorigenic properties and can be measured by using cell migration assays [41]. The average healing rates of OVCAR-3 and HO-8910 were significantly greater than that of HOSEpiC (Figure 2a,b), which is consistent with the changes of viscoelastic results by AFM, indicating the relationship between migratory potential and viscoelastic properties of ovarian cancer cells.…”

Section: Tumorigenic Properties Of Ovarian Cancer Cellssupporting

confidence: 74%

Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

Chen¹,

Zeng²,

Ruan³

et al. 2020

Beilstein J. Nanotechnol.

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The mechanical properties of cells could serve as an indicator for disease progression and early cancer diagnosis. This study utilized atomic force microscopy (AFM) to measure the viscoelastic properties of ovarian cancer cells and then examined the association with the invasion of ovarian cancer at the level of living single cells. Elasticity and viscosity of the ovarian cancer cells OVCAR-3 and HO-8910 are significantly lower than those of the human ovarian surface epithelial cell (HOSEpiC) control. Further examination found a dramatic increase of migration/invasion and an obvious decease of microfilament density in OVCAR-3 and HO-8910 cells. Also, there was a significant relationship between viscoelastic and biological properties among these cells. In addition, the elasticity was significantly increased in OVCAR-3 and HO-8910 cells after the treatment with the anticancer compound echinomycin (Ech), while no obvious change was found in HOSEpiC cells after Ech treatment. Interestingly, Ech seemed to have no effect on the viscosity of the cells. Ech significantly inhibited the migration/invasion and significantly increased the microfilament density in OVCAR-3 and HO-8910 cells, which was significantly related with the elasticity of the cells. An increase of elasticity and a decrease of invasion were found in OVCAR-3 and HO-8910 cells after Ech treatment. Together, this study clearly demonstrated the association of viscoelastic properties with the invasion of ovarian cancer cells and shed a light on the biomechanical changes for early diagnosis of tumor transformation and progression at single-cell level.

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“…In this context, plant extracts with healing activities have their effect justified by the presence of metabolites capable of capturing oxygen radicals [3,41,42]. The HECA extract showed an important concentration of phenolic compounds (209 mg / g GAE) confirming the potent Values are expressed as mean ± SEM and analyzed by one-way ANOVA followed by the tuckey-Kramer.…”

Section: Discussionmentioning

confidence: 99%

Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

et al. 2020

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Based on ethnopharmacological studies, a lot of plants, as well as its compounds, have been investigated for the potential use as wound healing agents. In Brazil, Curatella americana is traditionally used by local people to treat wounds, ulcers and inflammations. However, to the best of our knowledge, its traditional use in the treatment of wounds has not been validated by a scientific study. Here, some compounds, many of them flavonoids, were identified in the hydroethanolic extract from the leaves of C. americana (HECA) by LC-HRMS and LC-MS/MS. Besides that, solutions containing different concentrations of HECA and a gel produced with this extract were evaluated for its antimicrobial, coagulant and wound healing activities on an excision mouse wound model as well as its acute dermal safety. A total of thirteen compounds were identified in HECA, mainly quercetin, kaempferol and glucoside derivatives of both, besides catechin and epicatechin known as wound healing agents. The group treated with 1% of HECA exhibited highest wound healing activity and best rate of wound contraction confirmed by histopathology results. The present study provides scientific evidence of, this extract (HECA) possess remarkable wound healing activity, thereby, supporting the traditional use.

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Studies on wound healing potential of polyherbal formulation using in vitro and in vivo assays

Cited by 62 publications

References 21 publications

Association Examined of Viscoelastic Properties with the Invasion of Ovarian Cancer Cells by Atomic Force Microscopy

Association Examined of Viscoelastic Properties with the Invasion of Ovarian Cancer Cells by Atomic Force Microscopy

Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

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