STUDIES ON ULTRACENTRIFUGE FRACTIONS OF ANTERIOR PITUITARY WITH SPECIAL REFERENCE TO ADRENOCORTICOTROPHIC HORMONE¹

Biosynthesis of Adrenocorticotropin and Protein in Slices of Bovine Anterior Pituitary Tissue*

“…This observation is not surprising in view of the finding that vasopressin formation was also depressed by this same antibiotic (Haller et al, 1964). It may be pointed out at this juncture that ACTH was found distributed between microsomal and supernatant fractions in porcine anterior pituitary preparations (Brown and Ulvedal, 1960). The possibility therefore exists that the Zamecnik cycle of reactions governs the biosynthetic pathway of the several pituitary peptide hormones.…”

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

“…Brown and Ulvedal (1960) had localized TSH activity in the mitochondria and ACTH activity was found to be -118-dispersed over several subcellular fractions; however, no hormonal activity was found to be present in the nuclear fraction. Yet, one…”

“…Assaying subcellular fractions for hormonal activity is not an unprecedented investigative procedure since Brown and Ulvedal (1960) localized hormonal activity in a number of anterior pituitary fractions. Similarly, Hamilton and Cohn (1969) demonstrated that the 105,000 x g particulate fraction of parathyroid glands exhibited hypercalcemic activity which is characteristic of PTH.…”

The Cytological Localization of Acth in the Human Pituitary*

“…Among the methods for demonstrating the cellular sites of production of anterior pituitary hormones are special histochemical techniques correlated with clinical and experimental findings (1)(2)(3), ultracentrifugation studies (4,5), tissue culture methods (6), and fluorescent antibody methods (7)(8)(9). The fluorescent antibody technique has the distinct advantages of specificity and of being applicable to pituitaries in histologic section.…”