Biosynthesis of Adrenocorticotropin and Protein in Slices of Bovine Anterior Pituitary Tissue^*

Abstract

of the guinea pig. Mention may also be made of tissue slice experiments on the biosynthesis of the low molecular weight protein insulin (Humbel, 1963;Mallory et al., 1964).The bovine adrenocorticotropin (ACTH)1 molecule, with 39 amino acid residues (Li et al., 1961), was selected for the present investigation. 123456789 10 11Seryr-Ser-Met-Glu-His-Phe-Arg-T ry-Gly-Lys-12 13 14

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

“…In vitro biosynthetic media containing either slices or homogenates of such endocrine tissues as the hypothalamus (Takabatake and Sachs, 1964), anterior pituitary (Adiga et al, 1965), parathyroid glands (Hamilton andCohn, 1969 andHamilton !! al., 1971), and thyroid glands (Sorgente, 1969 andRadde et al, 1970) have been utilized to monitor the biosynthesis and release of hormones.…”

The small-scale isolation and characterization of adrenocorticotrophin

“…Chymotrypsin digestion was performed as described by Adiga et al (1965). The carboxypeptidase procedure of Harris & Li (1955) was used to identify C-terminal amino acids.…”

“…A numnber of techniques have been employed, with or without prior oxycellulose treatment, for the final purification of ACTH. These include chromatography on cation-exchange resins (Dixon, Moore, Stacke-Dunne & Young, 1951;White -& Fierce, 1953;Li et al 1954;Dixon & Stack-Dunne, 1955;White & Peters, 1956; Li & Dixon, 1956), countercurrent distribution (Li et al 1954;Li & Dixon, 1956;Shepherd et al 1956), CM-cellulose and DEAE-cellulose chromatography (Farmer, 1959;Lee, Lerner & Buettner-Janusch, 1960;De Jager, Homan & de Wied, 1963;Pickering, Anderson, Lohmar, Birk & Li, 1963), SE-Sephadex chromatography (Island et al 1965), Sephadex-gel filtration (Birk & Li, 1964), paper chromatography and paper electrophoresis (White, Fierce & Lesh, 1951;Jacobowitz, Marks & Vernikos-Danellis, 1963;Adiga, Uemura & Winnick, 1965). A recent technique (Papkoff & Li, 1966) has increased the customary yield from ovine pituitaries tenfold, to 100mg.…”

“…There is, however, need for convenient small-scale isolation and purification procedures for biosynthetic studies. The present study arose from experiments concerned with the incorporation ofradioactive amino acids into ACTH in surviving slices and cell-free ribosomal preparations derived from anterior pituitary tissue (Adiga et al 1965;Adiga, Rao, Hussa & Winnick, 1966). Its purpose was to compare several isolation procedures adapted to the scale of 1-20g.…”