Isolation of sheep anterior pituitary messenger RNA and its translation in a heterologous cell-free system

Prasad, Mahesh; Adiga, P. Radhakantha

doi:10.1007/bf02716881

Cited by 1 publication

(2 citation statements)

References 21 publications

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“…[22] Briefly, the obtained protein was emulsified with an equal proportion of Freund's complete adjuant (Sigma Aldrich) and injected subcutaneously at weekly intervals for 4 weeks into Rabbits at various sites. The rabbits were then bled through the ear veins 7 days after the completion of the booster dose.…”

Section: Production Of Antiserum To Riboflavin Binding Protein (Rbp)mentioning

confidence: 99%

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IMMUNOLOGICAL CHARACTERIZATION AND COMPARISON BETWEEN EGG YOLK & WHITE RIBOFLAVIN BINDING PROTEINS OF LARGEST FLIGHTLESS BIRD OSTRICH AND THE FLYING BIRD HEN

Nikhath¹

2017

WJPR

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Ostrich (Struthiocamelus), the largest flightless bird's and the flying bird (Gallus gallus) hen's egg Riboflavin Binding Protein (RBP) was isolated and purified from their eggs. First, the purification of RBP was undertaken in two steps, using DEAE-Sepharose ion exchange chromatography. Second, the purified (RBP) sample was obtained using gel filtration on Sephadex G-100. The holoprotein had an absorption spectrum characteristic of Flavoproteins. The purity of the RBP, was confirmed by SDS slab-gel electrophoresis, where the isolated RBP migrated as a single band. The mobility of the RBP with that of standard mol.wt proteins marker suggested that the mol.wt was 54 kda (Ostrich) and 29 kda (Hen). Against these RBP's antiserum was raised in Rabbits and this antiserum showed immunological cross reactivity -precipitin lines between the two compounds i.e flightless bird and flying bird RBPs. Hence the study proves that, the largest flightless bird (Ostrich) and flying bird (Hen) are immunologically relatives and phylogenetically different from each other.

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Section: Production Of Antiserum To Riboflavin Binding Protein (Rbp)mentioning

confidence: 99%

“…Antibodies against Ostrich (flightless bird) egg white and Hen (flying bird) egg yolk RBP were produced by adopting method of Prasad and Adiga [22] as described earlier in materials and methods. Studies on the immunological aspects of RBPS are very limited and hence detailed analysis is required.…”

Section: Production Of Antiserum Of Riboflavin Binding Proteinmentioning

confidence: 99%

IMMUNOLOGICAL CHARACTERIZATION AND COMPARISON BETWEEN EGG YOLK & WHITE RIBOFLAVIN BINDING PROTEINS OF LARGEST FLIGHTLESS BIRD OSTRICH AND THE FLYING BIRD HEN

Nikhath¹

2017

WJPR

View full text Add to dashboard Cite

show abstract

Isolation of sheep anterior pituitary messenger RNA and its translation in a heterologous cell-free system

Cited by 1 publication

References 21 publications

IMMUNOLOGICAL CHARACTERIZATION AND COMPARISON BETWEEN EGG YOLK & WHITE RIBOFLAVIN BINDING PROTEINS OF LARGEST FLIGHTLESS BIRD OSTRICH AND THE FLYING BIRD HEN

IMMUNOLOGICAL CHARACTERIZATION AND COMPARISON BETWEEN EGG YOLK & WHITE RIBOFLAVIN BINDING PROTEINS OF LARGEST FLIGHTLESS BIRD OSTRICH AND THE FLYING BIRD HEN

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