Studies on Tubercle Bacillus-Monocyte Relationship

Fong, Jacob; Chin, Dennis; Elberg, Sanford S.

doi:10.1084/jem.114.1.75

Cited by 10 publications

(4 citation statements)

References 8 publications

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“…In fact, Fong, Chin, and Elberg (1961) have recently demonstrated a series of changes which are impressed upon mycobacteria when they are passed directly through generations of intramonocytic growth. Losses in mouse lethality, neutral red binding, increased sensitivity to sodiumn oleate, among others, were determined.…”

Section: Discussionmentioning

confidence: 99%

Lysis of Brucellae by the Combined Action of Glycine and a Lysozyme-Like Agent From Rabbit Monocytes

1961

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of brucellae by the combined action of glycine and a lysozyme-like agent from rabbit monocytes. J. Bacteriol. 82:342-353. 1961.-An acidextractable lytic material was obtained from rabbit monocytes. It acts on a substrate in the walls of brucellae and has properties similar to egg-white lysozyme. Brucella melitensis strain Rev Is is completely resistant to this agent and also to crystalline lysozyme, but when the cells are exposed to sufficient amounts of glycine, the surface is rendered susceptible to these lytic agents. Rough type Rev Is cells are more susceptible than smooth, and the virulent B. melitensis strain 6015 is most resistant.

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Section: Discussionmentioning

confidence: 99%

Lysis of Brucellae by the Combined Action of Glycine and a Lysozyme-Like Agent From Rabbit Monocytes

1961

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“…The use of tissue culture methods (2,3) has yielded more controversial findings, but one of these earlier studies (2) appeared to confirm Lurie's conclusions concerning acquired cellular resistance. Our own investigations involving in vitro cultures of infected histiocytes have shown that peritoneal exudate cells of normal and immunized rabbits could modify virulent tubercle bacilli, thereby reducing bacillary virulence for mice and inducing changes in certain other bacterial attributes (4). Although these modifications of virulent tubercle bacilli occurred after interaction of bacilli with either normal or immune histiocytes, only immune histiocytes were able to alter virulent tubercle bacilli to the extent where they lost the ability to necrotize the histiocytes themselves (5); this latter observation may be indicative of a greater capacity of immune histiocytes to modify bacillary virulence.…”

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confidence: 99%

Studies on Tubercle Bacillus-Histiocyte Relationship

Fong

Chin

Elberg

1962

The Journal of Experimental Medicine

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Studies of passive transfer of cellular resistance, as manifested by refractoriness to necrotization with virulent tubercle bacilli, have shown that immune histiocytes or immune lymphocytes were effective transferring agents; immune polymorphonuclear leucocytes and immune serum as well as comparable cells from normal animals lacked this capacity. Comparisons of immune histiocytes and immune lymphocytes showed that the former cells were more efficient; this was indicated by (a) the smaller numbers of immune histiocytes needed for passive transfer, (b) the longer duration of cellular resistance in recipients given histiocytes than in those given lymphocytes, (c) the greater capacity of histiocytes to effect serial passive transfer, and (d) the ability of histiocytic but not lymphocytic lysates to transfer cellular resistance. Experiments to establish the mechanism of passive transfer of cellular resistance showed that there was no active induction of resistance in recipients through transfer of bacillary antigens contained in immune histiocytes; in fact, the results of serial passive transfers with immune histiocytes suggested an active replication of the "cell resistance factor."

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“…Detailed descriptions of procedures and materials used in these studies have been described in earlier reports (1)(2)(3)(4)(5)(6) and are therefore briefly described herein.…”

Section: Methodsmentioning

confidence: 99%

“…Preparation of Passaged Bacter/~.--The preparation of virulent tubercle bacilli which had been grown in immune histiocytes has been described in an earlier paper (6). The same methods were used in the present studies except that virulent H37Rv were grown in Brucdla-lmmune hlstiocytes rather than BCG-immune histiocytes.…”

Section: Mycobacterium Tuberculosismentioning

confidence: 99%

Studies of Tubercle Bacillus-Histiocyte Relationships

Fong

Chin

Elberg

1964

The Journal of Experimental Medicine

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Earlier reports (1, 2) from this laboratory have shown that immunization of animals with the BCG strain of tubercle bacillus resulted in the development of resistant populations of histiocytes in these animals; this form of cellular resistance was manifested by the refractoriness of infected immune histiocytes to necrotization by virulent tubercle bacilli (H37Rv strain). The expression of such resistance was, however, dependent upon the presence of some non-specific factor present in homologous and heterologous immune sera (e.g. anti-BCG, antiovalbumin, anti-Salmonella sera).Further studies (3) have shown that a similar type of cellular resistance was demonstrable with the histiocytes of rabbits immunized with the Rev I strain of Brucella mel/tens/s. There was, moreover, a state of cross-immunity between the Brucella-immune (from animals immunized with Rev I) and the BCG-immune (from animals immunized with BCG) histiocytes such that infection of one or the other of the two histiocytes with either the homologous or heterologous pathogen did not result in destruction of cells by the parasite. An obvious inference which may be drawn from this observation is the probable identity of the mechanism or mechanisms for this type of cellular resistance.Since the above findings have been reported, additional characterization of the tubercle bacillus-histiocyte model system has been achieved (4--6). It is now known that cellular resistance against mycobacteria can be induced in normal animals by injections of immune histiocytes, recipient histiocytes (histiocytes of normal animals which had been injected with resistant cells), and by immune ribosomes and ribosomal RNA (ribonucleic acid). It is also an established fact that serial transfer of resistance in normal animals against mycobacteria is achievable by injection of animals with resistant histiocytes or subfractions (ribosomes) derived therefrom. Moreover, it has been shown that passage of virulent tubercle bacilli in immune histiocytes resulted in attenuation of bacilli for both mice and normal histiocytes (i.e. passaged bacilli lose their inherent ability to necrotize the histiocytes of normal animals).In view of the above findings, it seemed propitious to investigate the identity or lack of identity of the basic mechanisms which underlie cellular immunity

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Studies on Tubercle Bacillus-Monocyte Relationship

Cited by 10 publications

References 8 publications

Lysis of Brucellae by the Combined Action of Glycine and a Lysozyme-Like Agent From Rabbit Monocytes

Lysis of Brucellae by the Combined Action of Glycine and a Lysozyme-Like Agent From Rabbit Monocytes

Studies on Tubercle Bacillus-Histiocyte Relationship

Studies of Tubercle Bacillus-Histiocyte Relationships

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