Studies on Tubercle Bacillus-Histiocyte Relationship

Fong, Jacob; Chin, Dennis; Elberg, Sanford S.

doi:10.1084/jem.115.3.475

Cited by 24 publications

(14 citation statements)

References 13 publications

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“…Recent reports on the passive transfer of acquired resistance in tuberculosis (7,8,11,12,34,36) can also be recognized as presentations of the cellular nature of immunity.…”

Section: Discussionmentioning

confidence: 99%

The Use of Diffusion Chambers in Investigating the Cellular Nature of Immunity in Experimental Typhoid and Tuberculosis

Akiyama

Maeda

Asaba

et al. 1964

Japanese Journal of Microbiology

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“…Recent reports on the passive transfer of acquired resistance in tuberculosis (7,8,11,12,34,36) can also be recognized as presentations of the cellular nature of immunity.…”

Section: Discussionmentioning

confidence: 99%

The Use of Diffusion Chambers in Investigating the Cellular Nature of Immunity in Experimental Typhoid and Tuberculosis

Akiyama

Maeda

Asaba

et al. 1964

Japanese Journal of Microbiology

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“…Detailed descriptions of procedures and materials used in these studies have been described in earlier reports (1)(2)(3)(4)(5)(6) and are therefore briefly described herein.…”

Section: Methodsmentioning

confidence: 99%

“…Details concerning the test for cellular resistance have been previously described (4). Briefly, the procedure was as follows: part of the histiocytes which had been collected from donor animals or recipient animals was sedimented by centrifugation and redispersed in a small volume of 0.25 per cent trypsin in Tyrode's solution.…”

Section: Methodsmentioning

confidence: 99%

“…Resistance in Normal Animals.--Previous investigations (4,5) have shown that the resistance of hisfiocytes against virulent tubercle bacilli was transferable to normal animals by injections of immune histiocytes or of ribosomes and ribosomal RNA derived from the immune histiocytes. It therefore seemed reasonable that if the mechanism of resistance of Brucella-immune cells resembled that of the BCG-immune cells, transference to normal animals of immunity against Brucella should be similarly achievable.…”

Section: Inability Of Brucdla-immune Histiocytes and Ribosomes To Indmentioning

confidence: 99%

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Studies of Tubercle Bacillus-Histiocyte Relationships

Fong

Chin

Elberg

1964

The Journal of Experimental Medicine

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Earlier reports (1, 2) from this laboratory have shown that immunization of animals with the BCG strain of tubercle bacillus resulted in the development of resistant populations of histiocytes in these animals; this form of cellular resistance was manifested by the refractoriness of infected immune histiocytes to necrotization by virulent tubercle bacilli (H37Rv strain). The expression of such resistance was, however, dependent upon the presence of some non-specific factor present in homologous and heterologous immune sera (e.g. anti-BCG, antiovalbumin, anti-Salmonella sera).Further studies (3) have shown that a similar type of cellular resistance was demonstrable with the histiocytes of rabbits immunized with the Rev I strain of Brucella mel/tens/s. There was, moreover, a state of cross-immunity between the Brucella-immune (from animals immunized with Rev I) and the BCG-immune (from animals immunized with BCG) histiocytes such that infection of one or the other of the two histiocytes with either the homologous or heterologous pathogen did not result in destruction of cells by the parasite. An obvious inference which may be drawn from this observation is the probable identity of the mechanism or mechanisms for this type of cellular resistance.Since the above findings have been reported, additional characterization of the tubercle bacillus-histiocyte model system has been achieved (4--6). It is now known that cellular resistance against mycobacteria can be induced in normal animals by injections of immune histiocytes, recipient histiocytes (histiocytes of normal animals which had been injected with resistant cells), and by immune ribosomes and ribosomal RNA (ribonucleic acid). It is also an established fact that serial transfer of resistance in normal animals against mycobacteria is achievable by injection of animals with resistant histiocytes or subfractions (ribosomes) derived therefrom. Moreover, it has been shown that passage of virulent tubercle bacilli in immune histiocytes resulted in attenuation of bacilli for both mice and normal histiocytes (i.e. passaged bacilli lose their inherent ability to necrotize the histiocytes of normal animals).In view of the above findings, it seemed propitious to investigate the identity or lack of identity of the basic mechanisms which underlie cellular immunity

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“…It has been established by many investigators that acquired resistance against a facultative intracellular parasite,Mycobacterium tuberculosis, depends upon cell-mediated immunity [11,14,18,25]. Experimental findings during the last decade have shown that the effector cells of this type of cellmediated immunity are immune macrophages [1,2,17,29].…”

mentioning

confidence: 99%

In Vitro Studies on the Mechanism of Acquired Resistance to Tuberculous Infection

Muraoka

Takeya

Nomoto

1976

Japanese Journal of Microbiology

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Immune lymph node cells were obtained from mice immunized with bovine gamma globulin (BGG) in complete Freund's adjuvant or allogeneic MH 134 tumor cells. They showed the capacity of conferring bactericidal activity on macrophages infected with Mycobacterium tuberculosis, H37Rv, when they were incubated on macrophage monolayers together with the corresponding antigen, i.e., BGG or solubilized cellular antigen of the tumor cells. However, such capacity was lower than that of tubercle bacilli-immune lymph node cells. Culture supernatants were harvested after incubation of tubercle bacilli-immune, BGG-immune or allogeneic tumor-immune lymph node cells with the corresponding antigen for 24 hr. Macrophages were altered so as to suppress intracellular bacillary growth when macrophage monolayers were exposed to the supernatants for more than 2 days. When normal lymph node cells were incubated on normal macrophage monolayers together with a mitogen such as PHA or concanavalin A, growth of tubercle bacilli within the macrophages was slightly but difinitely suppressed. The mechanism of elicitation of cellular immunity to the infection with tubercle bacilli is discussed on the basis of results presented in this and the preceding paper.

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Studies on Tubercle Bacillus-Histiocyte Relationship

Cited by 24 publications

References 13 publications

The Use of Diffusion Chambers in Investigating the Cellular Nature of Immunity in Experimental Typhoid and Tuberculosis

The Use of Diffusion Chambers in Investigating the Cellular Nature of Immunity in Experimental Typhoid and Tuberculosis

Studies of Tubercle Bacillus-Histiocyte Relationships

In Vitro Studies on the Mechanism of Acquired Resistance to Tuberculous Infection

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