Studies on TLR2 gene variants and their association with milk yield and milk quality traits in Bos indicus (Deoni) cattle

Abstract

Present study molecular characterization of exon 2 of TLR2 gene and its association with milk yield and milk quality traits in 104 Deoni cattle using PCR-RFLP technique was done. Polymorphism was observed through HaeIII, HhaI and EcoRV restriction enzymes in Created Restriction Site (CRS) exon 2-1, CRS exon 2-5 and exon 2-1 by PCR-RFLP, respectively. In CRS exon 2-1 allelic frequencies were observed as 0.793 for A and 0.206 for B alleles and that of genotypic frequencies were 0.58 and 0.41 for genotypes AA and AB. In CRS exon 2-5, two genotypes viz., AC and CC with corresponding allelic frequencies were observed as 0.221 for A and 0.778 for C allele and that of genotypic frequencies observed were 0.44 and 0.55 for AC and CC genotypes respectively. TLR2 exon 2-1 exhibited two alleles G and T with frequencies of 0.134 and 0.865 and their Corresponding genotypic frequencies were 0.009, 0.25 and 0.74for GG, GT and TT genotypes respectively. Higher count of somatic cells (SCC) in TT homozygous and TG heterozygous genotypes, and lower in GG homozygous genotypes were observed in exon 2-1. Strongly significant (P0.01) effect for least squares means of Test Day milk yield (TDMY) and Somatic Cell Count of CRS exon 2-1 were observed.

Teat Morphological and Milk Somatic Cell Comparison between Zebu Cattle (Deoni) and HF Crossbred (Bos taurus x Bos indicus)

“…The result showed that Deoni's milk had significantly lesser somatic cell count than HF crossbred's milk (Table .1). But the previous study on season-wise change of SCC average revealed higher values than this present study values (Mundhe et al, 2018), because in that previous investigation both infected and healthy animals were considered.…”

Peripheral blood concentration of toll-like receptor-4 and its accuracy for prediction of postpartum performances of transition zebu (Bos indicus) cows

“…Higher expression of TLR-2 and TLR-4 in mastitis affected animals was reported by Goldammer et al (2004) and Yang et al (2008). Mundhe et al (2018) also reported association of TLR-2 gene polymorphism with milk production in Deoni (Bos indicus) cows. Razak et al (2015) revealed significant association of TLR-4 gene polymorphism with mastitis in Indian dairy cows.…”

A Review on Production, Reproduction and Disease Resistance Traits on Deoni Cattle

“…Strongly significant (P≤0.01) effect for least squares means of Test Day milk yield (TDMY) and Somatic Cell Count of CRS exon 2-1 were observed. Hence polymorphisms were detected through PCR-RFLP in exon 2 of TLR2 gene using Hae III, HhaI and EcoRV restriction enzymes and their association with somatic cell count, test day milk yield, fat and solid not fat percentage in Deoni indigenous cows (Mundhe et al, 2018).…”