Studies on the Uganda I/CDC Strain of Plasmodium malariae in Bolivian Aotus Monkeys and Various Anophelines

We, Collins; Ik, Schwartz; Jc, Skinner; Broderson,

doi:10.2307/3281749

Cited by 17 publications

(5 citation statements)

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“…Banked sera from malaria studies conducted in experimentally infected chimpanzees prior to 2000 were included in this report. Chimpanzees Bit and Klimatis were infected with the Uganda I strain of P. malariae [ 46 , 47 ], Alpert was infected with the Nigeria I strain of P. ovale [ 48 ], and Duff was infected with the Salvador I strain of P. vivax [ 49 ]. As previously described, all animals had been splenectomized before they were inoculated intravenously with heparinized, infected blood.…”

Section: Methodsmentioning

confidence: 99%

Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Priest

Pluciński

Huber

et al. 2018

Malar J

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BackgroundMultiplex bead assays (MBA) that measure IgG antibodies to the carboxy-terminal 19-kDa sub-unit of the merozoite surface protein 1 (MSP119) are currently used to determine malaria seroprevalence in human populations living in areas with both stable and unstable transmission. However, the species specificities of the IgG antibody responses to the malaria MSP119 antigens have not been extensively characterized using MBA.MethodsRecombinant Plasmodium falciparum (3D7), Plasmodium malariae (China I), Plasmodium ovale (Nigeria I), and Plasmodium vivax (Belem) MSP119 proteins were covalently coupled to beads for MBA. Threshold cut-off values for the assays were estimated using sera from US citizens with no history of foreign travel and by receiver operator characteristic curve analysis using diagnostic samples. Banked sera from experimentally infected chimpanzees, sera from humans from low transmission regions of Haiti and Cambodia (N = 12), and elutions from blood spots from humans selected from a high transmission region of Mozambique (N = 20) were used to develop an antigen competition MBA for antibody cross-reactivity studies. A sub-set of samples was further characterized using antibody capture/elution MBA, IgG subclass determination, and antibody avidity measurement.ResultsTotal IgG antibody responses in experimentally infected chimpanzees were species specific and could be completely suppressed by homologous competitor protein at a concentration of 10 μg/ml. Eleven of 12 samples from the low transmission regions and 12 of 20 samples from the high transmission area had antibody responses that were completely species specific. For 7 additional samples, the P. falciparum MSP119 responses were species specific, but various levels of incomplete heterologous competition were observed for the non-P. falciparum assays. A pan-malaria MSP119 cross-reactive antibody response was observed in elutions of blood spots from two 20–30 years old Mozambique donors. The antibody response from one of these two donors had low avidity and skewed almost entirely to the IgG3 subclass.ConclusionsEven when P. falciparum, P. malariae, P. ovale, and P. vivax are co-endemic in a high transmission setting, most antibody responses to MSP119 antigens are species-specific and are likely indicative of previous infection history. True pan-malaria cross-reactive responses were found to occur rarely.Electronic supplementary materialThe online version of this article (10.1186/s12936-018-2566-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Priest

Pluciński

Huber

et al. 2018

Malar J

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“…Parasite and host samples and DNA extraction Two strains of Plasmodium malariae were isolated from patients who had acquired P. malariae infections in Uganda (Collins et al 1984) or Brazil (John Barnwell, unpublished data). The UgandaI/ CDC strain, after isolation from an infant infected by blood transfusion, was maintained by passage in splenectomized chimpanzees (Pan troglodytes) and New World monkeys.…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

“…Six were found to have homology to host DNA, 2 contained no repeats and 1 had a repeat at the end of the sequence. PCR primers were designed to the sequences of the other 27 clones and orthologous sequences were amplified and sequenced from the DNA from each of 2 laboratory isolates of P. malariae (Pm-Uganda I/CDC ; Collins et al 1984 and Pm-Brazil I). On the basis of observed and potential for polymorphism, 11 loci were selected for further use and analyses using a semi-nested PCR (see Table 2) employing a panel of 76 test samples of P. malariae from a wide geographical range, 2 P. brasilianum isolates and 15 controls including other Plasmodium species from humans and host DNA samples (Table 1).…”

Section: R E S U L T Smentioning

confidence: 99%

Characterization and application of multiple genetic markers forPlasmodium malariae

et al. 2006

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SUMMARYPlasmodium malariae, a protozoan parasite that causes malaria in humans, has a global distribution in tropical and subtropical regions and is commonly found in sympatry with other Plasmodium species of humans. Little is known about the genetics or population structure of P. malariae. In the present study, we describe polymorphic genetic markers for P. malariae and present the first molecular epidemiological data for this parasite. Six microsatellite or minisatellite markers were validated using 76 P. malariae samples from a diverse geographical range. The repeat unit length varied from 2 to17 bp, and up to 10 different alleles per locus were detected. Multiple genotypes of P. malariae were detected in 33 of 70 samples from humans with naturally acquired infection. Heterozygosity was calculated to be between 0·236 and 0·811. Allelic diversity was reduced for samples from South America and, at some loci, in samples from Thailand compared with those from Malawi. The number of unique multilocus genotypes defined using the 6 markers was significantly greater in Malawi than in Thailand, even when data from single genotype infections were used. There was a significant reduction in the multiplicity of infection in symptomatic infections compared with asymptomatic ones, suggesting that clinical episodes are usually caused by the expansion of a single genotype.

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“…20,2007 PLASMODIUM MALARIAE: PARASITE AND DISEASE 587 monkeys was reported by Geiman and Siddiqui (49). Additional studies were made with different species of Aotus and Saimiri monkeys (23,28,32,33,34,36). In splenectomized Aotus monkeys, maximum parasite counts with P. malariae varied markedly, from 10 to 56,800/l.…”

Section: Infections In Chimpanzees and Monkeysmentioning

confidence: 99%

Plasmodium malariae : Parasite and Disease

2007

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SUMMARY A review of the life history of Plasmodium malariae, the quartan malaria parasite of humans, is presented. Much of the information is based on data obtained from induced infections in humans who were given malaria therapy for the treatment of neurosyphilis between 1940 and 1963. Prepatent periods (i.e., the time until the first day of parasite detection) fever episodes, and maximum parasitemias as a result of infection with P. malariae were obtained and are presented. Experimental and known vectors of the parasite are also discussed. Splenectomized chimpanzees and New World monkeys are readily infected and serve as sources of parasites and antigens for diagnostic and molecular studies. South American monkeys are naturally infected with a parasite known as Plasmodium brasilianum. This parasite appears to be P. malariae that has adapted from humans to grow in monkeys, probably within the last 500 years. Infection with P. malariae is associated with the production of immune complexes in the kidneys and the associated nephrotic syndrome. The essential lesions are a thickening of the glomerular basement membrane and endocapillary cell proliferation. Studies of monkeys infected with P. malariae indicate the same pathology as that demonstrated in humans.

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Studies on the Uganda I/CDC Strain of Plasmodium malariae in Bolivian Aotus Monkeys and Various Anophelines

Cited by 17 publications

References 7 publications

Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Characterization and application of multiple genetic markers forPlasmodium malariae

Plasmodium malariae : Parasite and Disease

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