Studies on the Thermophilic Sulfate-Reducing Bacteria From a Souring North Sea Oil Field

Cochrane, W.J.; Jones, P.S.; Sanders, P.F.; Holt, D.M.; Mosley, M.J.

doi:10.2523/18368-ms

Cited by 9 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optimum temperature for sulfate reduction in colder sediments was around 30°C. 88il°8 Cochrane et al 19 observed that the presence and growth of thermophilic SRB at temperatures greater than 60°C was a major source of sulfide production in a North Sea oil field. None of the presently known sulfate reducers is able to grow at extremely high temperatures near 100°C or below -5°C.…”

Section: Temperaturementioning

confidence: 99%

Sulfate‐reducing bacteria

Hao

Chen

Huang

et al. 1996

Critical Reviews in Environmental Science and Technology

275

148

View full text Add to dashboard Cite

The corrosion of sewers and the control of odor are the major operational and maintenance problems in wastewater collection systems. The generation of hydrogen sulfide and subsequent sulfuric acid results from microbially mediated reactions, by sulfate-reducing bacteria (SBR) and sulfide-oxidizing bacteria. This review covers pertinent information about sulfate reduction-induced problems in general and SBR in particular. Metabolism with respect to carbon, energy, and sulfur sources, ecology, growth factors (dissolved oxygen, temperature, pH, and sulfide), and the competitive effects of methane-producing bacteria on SBR are discussed. Because metals react with sulfide to form metal sulfide precipitates with extremely low solubilities, metal interactions in sulfate reduction environments are discussed.

show abstract

Section: Temperaturementioning

confidence: 99%

Sulfate‐reducing bacteria

Hao

Chen

Huang

et al. 1996

Critical Reviews in Environmental Science and Technology

275

148

View full text Add to dashboard Cite

show abstract

“…Samples for microbial analysis were collected in 500 ml sterilised glass Duran bottles after 0, 2, 6, 12, 30, 48, 60, 78 and 96 h backXowing from well A-42 and after ¡0. 5,1,4,8,24,36,54,72 and 95 h backXowing from well B-26. The sample collected at the time ¡0.5 h contained water that had reached well-bottom but not the reservoir.…”

Section: Samplingmentioning

confidence: 99%

“…The introduction of sea water alters the physical and chemical conditions in the reservoir as sulphate-rich sea water gradually cools the reservoir formation [1,2] and blends with the warm (60-200°C) indigenous reservoir water (formation water) which is low in sulphate and rich in organic acids [3][4][5]. The cooling of the reservoir formation creates a new biotope for mesophilic sea water bacteria in the near injector area.…”

Section: Introductionmentioning

confidence: 99%

Microbial analysis of backflowed injection water from a nitrate-treated North Sea oil reservoir

Bødtker

Lysnes²,

Torsvik³

et al. 2009

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Reservoir souring in offshore oil fields is caused by hydrogen sulphide (H(2)S) produced by sulphate-reducing bacteria (SRB), most often as a consequence of sea water injection. Biocide treatment is commonly used to inhibit SRB, but has now been replaced by nitrate treatment on several North Sea oil fields. At the Statfjord field, injection wells from one nitrate-treated reservoir and one biocide-treated reservoir were reversed (backflowed) and sampled for microbial analysis. The two reservoirs have similar properties and share the same pre-nitrate treatment history. A 16S rRNA gene-based community analysis (PCR-DGGE) combined with enrichment culture studies showed that, after 6 months of nitrate injection (0.25 mM NO(3) (-)), heterotrophic and chemolithotrophic nitrate-reducing bacteria (NRB) formed major populations in the nitrate-treated reservoir. The NRB community was able to utilize the same substrates as the SRB community. Compared to the biocide-treated reservoir, the microbial community in the nitrate-treated reservoir was more phylogenetically diverse and able to grow on a wider range of substrates. Enrichment culture studies showed that SRB were present in both reservoirs, but the nitrate-treated reservoir had the least diverse SRB community. Isolation and characterisation of one of the dominant populations observed during nitrate treatment (strain STF-07) showed that heterotrophic denitrifying bacteria affiliated to Terasakiella probably contributed significantly to the inhibition of SRB.

show abstract

“…Mesophilic, thermophilic and hyperthermophilic SRB´s can grow at different temperatures and therefore colonize different parts of the reservoir. (5) However, in most situations the reservoir harsh environment does not support expressive microbiological activity unless the cool-, deareated-, rich in sulfate-, and SRB-containing-seawater is allowed to penetrate the reservoir and create a moving front (interface) with the formation fluid. In fact, the cooling of the reservoir fluid and the drop in salinity promoted by the mixing of the two waters, the existence of low molecular weight fatty acids and the anaerobic conditions found in the reservoir, are the factors that can bring about a window for BRS colonies to settle downhole.…”

Section: How Does H 2 S Form? • Anthropogenic Souringmentioning

confidence: 99%

“…So, if the bisulfite dosage is well controlled it is very unlikely that it ends up being a major source of sulfate in the reservoir, although sulfite ions are more easily reduced than sulfate ions (to sulfide ions) by SRB. (5) On the Use of Mass Spectrometry Analysis to Identify the H 2 S Genesis MS is a useful analytical tool used to identify and quantify known chemical compounds. Detection of chemical species in very small quantities is one of the amazing features presented by this technique.…”

Section: Hso 3 -+ ½ O 2 So 4 = + H +mentioning

confidence: 99%

Assessment of Oilfield Souring Mechanisms by Mass Spectrometry Analysis of the Stable Sulfur Isotopes Ratio

Martins¹,

Marques²

2006

Proceedings of First International Oil Conference and Exhibition in Mexico

View full text Add to dashboard Cite

TX 75083-3836, U.S.A., fax 01-972-952-9435. AbstractReservoir souring is defined as the increase mass of gaseous Hydrogen Sulfide (H 2 S) per unit mass of produced fluids. Unless the onset of H 2 S takes place either during the appraisal of the well or in the very early stages of oil and/or gas production, it is not quite straightforward to identify the origin of souring. Under reservoir conditions, H 2 S is extremely reactive thus being able to react with native H 2 Ssequestering agents and be converted to metallic sulfides, elemental sulfur or organic sulfur compounds. This phenomenon can account for the delay of the onset of souring in the producer wells.It is a common practice in offshore facilities to prepare the drilling, completion and work-over fluids using seawater which is rich in sulfate and contains sulfate-reducing-bacteria (SRB). Should these fluids be lost to formation there is potential of (anthropogenic) reservoir souring promoted by these bacteria. In most situations, these fluids have always to bear the blame of reservoir souring (and many other problems). But it is not always correct to blame these fluids, because huge amounts of treated seawater are also injected in the formation (water flooding) for secondary recovery. Besides, H 2 S may be derived from non-anthropogenic sources. Consequently, a given souring problem may be derived from different sources and the existence of multiple possibilities render the souring identification a kind of difficult sometimes. But, this problem can be solved with the aid of instrumental analytical tools.The sulfur isotopic ratio ( 34 S/ 32 S) determination by Mass Spectrometry (MS) has been used to identify the H 2 S genesis, a key to properly address this controversial issue. This paper focuses on the use of this analytical tool to determine the sulfur isotope ratio and, therefore, to identify the source of souring. This paper also presents case histories on the use of MS to address souring problems that took place after workover jobs done in Campos Basin. Some aspects of reservoir souring mechanisms and SRB behavior are discussed as well.

show abstract

Studies on the Thermophilic Sulfate-Reducing Bacteria From a Souring North Sea Oil Field

Cited by 9 publications

References 0 publications

Sulfate‐reducing bacteria

Sulfate‐reducing bacteria

Microbial analysis of backflowed injection water from a nitrate-treated North Sea oil reservoir

Assessment of Oilfield Souring Mechanisms by Mass Spectrometry Analysis of the Stable Sulfur Isotopes Ratio

Contact Info

Product

Resources

About