Studies on the role of gelatin as a cryo- and lyo-protectant in the stability of capsicum oleoresin nanocapsules in gelatin matrix

Bejrapha, Piyawan; Surassmo, Suvimol; Choi, Mi‐Jung; Nakagawa, Kyuya; Min, Sang-Gi

doi:10.1016/j.jfoodeng.2011.02.042

Cited by 19 publications

(8 citation statements)

References 42 publications

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“…The breakdown of electrostatic interaction or osmotic shrinkage caused by an increase of salt concentration may also occur during freezing and lead to particle aggregation after thawing. Many studies have focused on the use of excipients such as cryoprotectants to protect nanoparticles during freezing process . Thus, we further examined the stability of AITC‐NPs during freezing–thawing processes.…”

Section: Resultsmentioning

confidence: 99%

“…Many studies have focused on the use of excipients such as cryoprotectants to protect nanoparticles during freezing process. 26 Thus, we further examined the stability of AITC-NPs during freezing-thawing processes. Figure 1(E) shows the cryostability of AITC-NPs treated with 1, 2, and 3 freezing-thawing cycles, as evident by a mean particle size of 10.2 ± 0.8, 9.9 ± 0.3 and 9.9 ± 0.2 nm, respectively, all of which were not significantly different when compared with the control treatment (9.5 ± 0.3 nm).…”

Section: Characterization and Stability Of Nanoparticlesmentioning

confidence: 99%

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Preparation of allyl isothiocyanate nanoparticles, their anti‐inflammatory activity towards RAW 264.7 macrophage cells and anti‐proliferative effect on HT1376 bladder cancer cells

et al. 2019

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BACKGROUND Allyl isothiocyanate (AITC), a volatile and water‐insoluble compound present in several cruciferous vegetables, has been shown to possess several biological qualities such as anti‐bacterial, anti‐fungal, and anti‐cancer activity. In this study, water‐soluble allyl isothiocyanate nanoparticles (AITC‐NPs) were prepared by oil dispersed in water (O/W) microemulsion and complex coacervation techniques and evaluated for their anti‐inflammatory activity towards macrophage cell RAW 264.7 and anti‐cancer effect on human bladder cancer cell HT1376. RESULTS The AITC‐NPs with a particle size of 9.4 nm were stable during heating up to 110 °C or three freeze‐thawing cycles. No significant cytotoxicity was shown on Caco‐2 and intestine epithelial IEC‐6 cells at AITC‐NP doses ranging from 0.25 to 2 g L−1 (8.75–70 mg L−1 AITC). However, at 2 g L−1 dosage, AITC‐NPs could inhibit the growth of human bladder cancer cells HT1376 by 90%, while their low dosage at 0.25 g L−1 could inhibit migration ability by 83.7, 71.3, 58.4 and 31.4% after 4, 8, 12, and 24 h of incubation, respectively. Compared to AITC and NPs, AITC‐NPs showed a better inhibition on lipopolysaccharide (LPS)‐induced TNF‐α, IL‐6, NO and iNOS production in RAW 264.7 macrophage cells. CONCLUSION The results demonstrate the potential of AITC‐NPs as therapeutic agents for the treatment of bladder cancer and the enhancement of immune function. © 2018 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Characterization and Stability Of Nanoparticlesmentioning

confidence: 99%

Preparation of allyl isothiocyanate nanoparticles, their anti‐inflammatory activity towards RAW 264.7 macrophage cells and anti‐proliferative effect on HT1376 bladder cancer cells

et al. 2019

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“…Though cryogels that underwent incubation always showed a larger size than their non-incubated counterparts, the effect of incubation was dominant for set 1 at a slower cooling rate of 0.5℃/min and for set 2 at 2.0℃/ min. The results may be attributed to the exposure of amino groups ) from the network by the formation of threedimensional cross-linked network during low-temperature incubation (Bejrapha et al, 2011). Prego et al (2006) reported that an increase in particle size indicated the attachment of polymers to the surface of the oil core.…”

Section: Effect Of Concentration Ratio Of κ-Carrageenan To Cmc On Sizmentioning

confidence: 99%

Encapsulation of Curcumin Loaded Oil Droplets with Chitosan Based Cryogel: Influence of Freezing Condition on Nanocapsule Properties

Sowasod

Nakagawa

Charinpanitkul

et al. 2013

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Encapsulation of curcumin-loaded oil droplets with chitosan-based cryogels, made from a ternary system of colloidal chitosan, κ-carrageenan, and carboxymethylcellulose (CMC) sodium salt, was carried out and the effects of the freezing condition (unidirectional freezing rate with and without post-freezing incubation) and the initial concentration ratio of carrageenan to CMC on the properties of the cryogel nanocapsules were experimentally investigated. Both the freezing condition and initial concentration ratio had significant effects on the gel fraction yield, swelling extent, particle size and zeta potential, as well as the curcumin release pattern. Specimen I-S1-02, which was made of polymer set 1 (carrageenan:CMC = 4:6) at a rapid freezing rate of −2℃/min with 10-hr incubation at −10℃, showed the slowest release rate (first-order type) of curcumin. In comparison, it was found that microcapsules obtained by spray-drying a similar O/W curcumin emulsion with dextran as stabilizer exhibited the fastest rate of curcumin release, similar to a burst release.

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“…[5][6][7] Freeze drying is a promising alternative drying method for stabilizing microcapsules in a matrix, called a stabilizer or lyoprotectant. [4,[8][9][10][11][12][13] Freeze drying is preferred over other drying methods because it dries materials in their original shapes and ensures higher stability of active ingredients. [14,15] The original shape of nano=microcapsules is a critical factor that determines the functional properties of the food; therefore, it is desirable to minimize the change in capsule size during the drying process.…”

Section: Introductionmentioning

confidence: 99%

“…[16] Our former investigations showed that gelatin is a promising candidate for stabilizing a colloidal vesicular system via freeze drying. [12,13] The gel network of gelatin helps to avoid unfavorable denaturation of the microcapsules and produces excellent dispersion characteristics in the dried matrix. [11] The dispersion characteristics can be controlled by the freezing conditions because polymeric network formation is strongly related to the freezing operation.…”

Section: Introductionmentioning

confidence: 99%

Stabilization of Microcapsules Using a Freeze-Dried Gelatin Matrix: Aqueous Redispersibility and the Ingredient Activity

et al. 2012

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Microcapsules are of great interest to the food and pharmaceutical industries as vehicles to deliver active ingredients to the gastrointestinal tract. Drying plays an important role in stabilizing microcapsules to prolong their lifetime; however, drying often produces undesirable changes in the microcapsules, such as irreversible aggregation of the microcapsules and activity loss of the encapsulated ingredient. In this work, poly(epsilon-caprolactone) microcapsules containing a model bioactive compound (tocopherol) were prepared and stabilized in a freeze-dried gelatin matrix. This dried product was rehydrated and the aqueous redispersibility of the microcapsules and the tocopherol activity were investigated. The experimental results suggested that a kinetic balance between dehydration (caused by freezing) and gel network formation is a critical factor that affects the redispersibility and ingredient activity of the products. It was further suggested that a hydrogel-based product could be strategically freeze dried to maximize product quality by tuning its freezing process; that is, by employing a controllable dehydration process.

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Studies on the role of gelatin as a cryo- and lyo-protectant in the stability of capsicum oleoresin nanocapsules in gelatin matrix

Cited by 19 publications

References 42 publications

Preparation of allyl isothiocyanate nanoparticles, their anti‐inflammatory activity towards RAW 264.7 macrophage cells and anti‐proliferative effect on HT1376 bladder cancer cells

Preparation of allyl isothiocyanate nanoparticles, their anti‐inflammatory activity towards RAW 264.7 macrophage cells and anti‐proliferative effect on HT1376 bladder cancer cells

Encapsulation of Curcumin Loaded Oil Droplets with Chitosan Based Cryogel: Influence of Freezing Condition on Nanocapsule Properties

Stabilization of Microcapsules Using a Freeze-Dried Gelatin Matrix: Aqueous Redispersibility and the Ingredient Activity

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