Studies on the preparations of cellulase produced by Irpex lacteus Fr. II. Properties of partially purified cellulolytic and plant tissue macerating enzymes of Irpex lacteus Fr. in special reference to their application.

Noguchi, Sadao; Shimura, Gen; Kawai, Masanobu; Suga, Yoshito; Samejima, Hirotoshi

doi:10.1271/bbb1961.42.339

Cited by 9 publications

(3 citation statements)

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“…Protoplasts were isolated from protonemal tissue using Driselase, a natural enzyme mixture containing laminarinase, xylanase and cellulase activities (Noguchi et al, 1978). This process imposes stress on plant cells, due to both loss of the cell wall and the effects of Driselase itself.…”

Section: Resultsmentioning

confidence: 99%

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

et al. 2016

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Plant protoplasts are widely used for genetic manipulation and functional studies in transient expression systems. However, little is known about the molecular pathways involved in a cell response to the combined stress factors resulted from protoplast generation. Plants often face more than one type of stress at a time, and how plants respond to combined stress factors is therefore of great interest. Here, we used protoplasts of the moss Physcomitrella patens as a model to study the effects of short-term stress on the chloroplast proteome. Using label-free comparative quantitative proteomic analysis (SWATH-MS), we quantified 479 chloroplast proteins, 219 of which showed a more than 1.4-fold change in abundance in protoplasts. We additionally quantified 1451 chloroplast proteins using emPAI. We observed degradation of a significant portion of the chloroplast proteome following the first hour of stress imposed by the protoplast isolation process. Electron-transport chain (ETC) components underwent the heaviest degradation, resulting in the decline of photosynthetic activity. We also compared the proteome changes to those in the transcriptional level of nuclear-encoded chloroplast genes. Globally, the levels of the quantified proteins and their corresponding mRNAs showed limited correlation. Genes involved in the biosynthesis of chlorophyll and components of the outer chloroplast membrane showed decreases in both transcript and protein abundance. However, proteins like dehydroascorbate reductase 1 and 2-cys peroxiredoxin B responsible for ROS detoxification increased in abundance. Further, genes such as thylakoid ascorbate peroxidase were induced at the transcriptional level but down-regulated at the proteomic level. Together, our results demonstrate that the initial chloroplast reaction to stress is due changes at the proteomic level.

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Section: Resultsmentioning

confidence: 99%

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

et al. 2016

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“…In the majority of published protocols Driselase is used dissolved in 1× MTSB buffer with pH of approximately 7.0 [ 3 ]. These conditions are suboptimal, because Driselase has quite low cell maceration activities and its pectolytic and cellulolytic activities have an optimum pH ranging from 4.0 to 6.0 and from 3.0 to 5.0, respectively [ 11 ]. In order to improve the cell wall digestion and increase tissue maceration a mixture of Driselase and Macerozyme R10 was used in MES buffer with pH 5.0.…”

Section: Methodsmentioning

confidence: 99%

Protocol: an improved and universal procedure for whole-mount immunolocalization in plants

et al. 2015

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Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner cell layers, tissues and organs still represents a major challenge for immunolocalization studies. Here we describe a protocol for whole-mount immunolocalization of proteins which is applicable to a wide range of plant species. The protocol is improved and robust for optimal sample fixation, tissue clearing and multi-protein staining procedures and can be used in combination with simultaneous detection of specific sequences of nucleic acids. In addition, cell wall and nucleus labelling can be implemented in the protocol, thereby allowing a detailed analysis of morphology and gene expression patterns with single-cell resolution. Besides enabling accurate, high resolution and reproducible protein detection in expression and localization studies, the procedure takes a single working day to complete without the need for robotic equipment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-015-0094-2) contains supplementary material, which is available to authorized users.

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“…The macerating enzymes used were Driselase (Kyowa Hakko Kogyo Co., Tokyo), a product of Basidiomycete fungi with activities similar to cellulase, pectinase, glucanase, xylanase, amylase, and others (Noguchi et al, 1978), and Cellulase Onozuka FA (Yakult Biochemicals Co., Nishinomiya, Japan), a product of Trichoderma viride Pers. ex Gray with activities similar to cellulase, protopectinase, hemicellulase, and others.…”

mentioning

confidence: 99%

Improvement of Rose Achene Germination by Treatment with Macerating Enzymes

Yambe¹,

Takeno²

1992

HortSci

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The germination percentage of Rosa multiflora Thunb. achenes was greatly increased when they were treated with 1% Driselase, a macerating enzyme, for 36 hours. The seeds germinated more rapidly when the achenes were treated with the enzyme for a longer period. Treatment with Cellulase Onozuka improved seed germination at a lower concentration than did Driselase. Pure preparations of pectinase and cellulase had effects similar to treatment with the enzymes noted. Treatment with pectinase was more efficient than treatment with cellulase. These enzymes likely loosened the bond between cells along the suture of the pericarp and forced the pericarp to split.

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Studies on the preparations of cellulase produced by Irpex lacteus Fr. II. Properties of partially purified cellulolytic and plant tissue macerating enzymes of Irpex lacteus Fr. in special reference to their application.

Cited by 9 publications

References 0 publications

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

Protocol: an improved and universal procedure for whole-mount immunolocalization in plants

Improvement of Rose Achene Germination by Treatment with Macerating Enzymes

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