Studies on the plasma membrane of normal and psoriatic keratinocytes. 3. Uptake of labelled sugars and their incorporation into glycoconjugates

Roelfzema, H.; Bergers, Mieke; Erp, P.E.J. van; Gommans, J.M.; Mier, P.D.

doi:10.1111/j.1365-2133.1981.tb00749.x

Cited by 15 publications

(7 citation statements)

References 4 publications

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“…By far the most interesting observation arising from the present work is the abnormality in the molecular weight distribution of fucose-containing glycopeptides, particularly since this confirms our previous report of a specific disturbance in the metabolism of fucose by psoriatic keratinocytes (Roelfzema et al, 1981). The reduced C/B ratio in the untreated lesion indicates either that the fucosylated glycopeptides are more highly branched than normal or that there is 5i6 H. Roelfzema et al an increased sialylation of glycopeptides with a normal degree of branching.…”

Section: Discussionsupporting

confidence: 91%

“…), fetuin, transferrin and thyroglobulin 510 H. Roelfzema et al from the Sigma Chemical Co., Ltd (St Louis, Miss., U.S.A.). Authentic samples of lipids were provided by Applied Science Laboratories, Inc., Philadelphia, U.S.A. All other chemicals were as described previously (Roelfzema et al, 1981). The selection of healthy volunteers and psoriatic patients, method of biopsy and preparation of keratinocyte suspensions were as reported by Gommans et al (1979).…”

Section: Methodsmentioning

confidence: 99%

“…Incubation of cells with radioactive sugars and the subsequent isolation of a *TCA-inso!ubIe sugar' pellet was as before (Roelfzema et al., 1981) using a 3 h or 22 h labelling period.…”

Section: Labelling and Isolation Proceduresmentioning

confidence: 99%

“…In general this was as described previously (Roelfzema et al., 1981). Samples of silica gel were scraped from thin-layer plates, suspended in i ml water and mixed with 10 ml 'Aquasol' for scintillation counting.…”

Section: Measurement Of Radioactivitymentioning

confidence: 99%

“…The uptake of labelled sugars by keratinocytes and their subsequent incorporation into glycoconjugates (glycoprotein, glycolipid and proteoglycan) have already been described (Roelfzema et al, 1981). Certain differences between normal and 'psoriatic' keratinocytes were observed, in particular in the metabolism of fucose.…”

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confidence: 91%

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Studies on the plasma membrane of normal and psoriatic keratinocytes. 4. Characterization of glycoconjugates

et al. 1981

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A substantial proportion (20-50%) of radioactive sugar incorporated into glycoconjugates by normal human keratinocytes was soluble in chloroform-methanol; using (14C)-galactose as precursor about half of this fraction was neutral lipid. The incorporation of labelled sugars into the lipid fraction was consistently increased in keratinocytes derived from psoriatic lesions. The abnormality in fucose metabolism which we reported previously has been confirmed. In particular we have shown that the molecular weight of fucosylated glycopeptides appears to be abnormally high in the untreated psoriatic lesion, possibly reflecting an increased degree of branching. In psoriatic uninvolved epidermis and in treated psoriatic lesions the situation is reversed, the molecular weight being significantly lower than normal.

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

“…Incubation of cells with radioactive sugars and the subsequent isolation of a *TCA-inso!ubIe sugar' pellet was as before (Roelfzema et al., 1981) using a 3 h or 22 h labelling period.…”

Section: Labelling and Isolation Proceduresmentioning

confidence: 99%

Section: Measurement Of Radioactivitymentioning

confidence: 99%

mentioning

confidence: 91%

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Studies on the plasma membrane of normal and psoriatic keratinocytes. 4. Characterization of glycoconjugates

et al. 1981

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Glycoconjugate biosynthesis in human epidermis

1987

Arch Dermatol Res

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Epidermal glycoconjugates synthesized by skin slices in media containing radiolabelled sugars were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A large proportion of the epidermal glycoconjugates were soluble in 10% (w/v) trichloroacetic acid. In addition to glycolipids and glycosaminoglycans, the acid-soluble fraction contained glycoproteins, a group of which (mol. wt. 70,000-160,000) were highly glycosylated and particularly rich in fucose. Analysis of the glycosaminoglycans by cellulose acetate electrophoresis combined with specific enzymic and nitrous acid degradation studies revealed that hyaluronic acid (73%) and heparan sulphate (18%) were the main components.

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The Binding of Helix pomatia and Ulex europeus Agglutinins to Normal and Psoriatic Skin

Mansbridge¹,

Knapp²

1984

Journal of Investigative Dermatology

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We have compared the staining patterns of Ulex europeus agglutinin (UEA) I-FITC and Helix pomatia agglutinin (HPA)-FITC on normal and psoriatic epidermis in order to follow the production of the binding sites as a function of maturation. We have further characterized them with respect to solvent extraction and enzyme digestion. The bandlike pattern of membrane staining by UEA I in the upper spinous and granular layer cells of normal epidermis is lost in psoriasis. Instead there is a fainter cytoplasmic staining which is largely sensitive to chloroform/methanol extraction, and thus presumably is glycolipid in nature. Epidermal binding of HPA to normal skin sections is mainly seen in cell membranes. It spares the basal layer and then increases from the suprabasal region to the granular layer. HPA staining in the spinous layer is destroyed by extraction with Triton X-100. In contrast, binding to the membranes of cells in psoriatic epidermis is triton-resistant. The result indicates the production of a component early in the maturation pathway which has no counterpart in normal skin. HPA binding to the spinous cells of symptomless skin from psoriatic patients shows decreased sensitivity to Triton X-100 by comparison with normal. The results are discussed in terms of changes in the pathway of keratinocyte maturation in psoriasis.

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Studies on the plasma membrane of normal and psoriatic keratinocytes. 3. Uptake of labelled sugars and their incorporation into glycoconjugates

Cited by 15 publications

References 4 publications

Studies on the plasma membrane of normal and psoriatic keratinocytes. 4. Characterization of glycoconjugates

Studies on the plasma membrane of normal and psoriatic keratinocytes. 4. Characterization of glycoconjugates

Glycoconjugate biosynthesis in human epidermis

The Binding of Helix pomatia and Ulex europeus Agglutinins to Normal and Psoriatic Skin

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