Glycoconjugate biosynthesis in human epidermis

Gp, Roberts

doi:10.1007/bf00417324

Arch Dermatol Res

1987

DOI: 10.1007/bf00417324

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Glycoconjugate biosynthesis in human epidermis

Roberts Gp

Abstract: Epidermal glycoconjugates synthesized by skin slices in media containing radiolabelled sugars were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A large proportion of the epidermal glycoconjugates were soluble in 10% (w/v) trichloroacetic acid. In addition to glycolipids and glycosaminoglycans, the acid-soluble fraction contained glycoproteins, a group of which (mol. wt. 70,000-160,000) were highly glycosylated and particularly rich in fucose. Analysis of the glycosaminoglycans by cel… Show more

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Cited by 3 publications

References 21 publications

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Influence of glycosaminoglycans on neurite morphology and outgrowth patterns in vitro

Verna

Fichard

Saxod

1989

Intl J of Devlp Neuroscience

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The neuritic growth patterns obtained on substrates made of several glycosaminoglycans (GAGs) bound to type I collagen were analysed and compared in primary cultures of chick embryo dorsal root ganglion grown in serum-free supplemented medium. In 2-day cultures grown on type I collagen or heparan sulphate (HS)-collagen surfaces, ganglionic explants exhibit a dense, symmetrical network of long, parallel neuritic processes and very few flat migrating non-neuronal cells. In contrast, on either dermatan sulphate (DS), chondroitin-6-sulphate (C6S) or hyaluronic acid (HA)-bound collagen substrates, neurons form irregular nerve fibre patterns; indeed, neurites follow convoluted paths and often, after abrupt turns, totally reverse their direction of extension. Experiments were carried out in which a choice was given to growing neural processes between collagen or GAG-collagen substrates. While growth cones elongating over type I collagen easily cross the border with HS-bound collagen surface and indiscriminately extend on this substrate, in contrast, neurites generally avoid surfaces coated with DS, C6S or HA and change their direction of growth in order to stay on collagen. The binding of DS, C6S or HA, but not HS, to type I collagen thus decreases its ability to promote neurite elongation. The interaction of neuronal cells with these extracellular matrix components by restricting neurites in their paths of extension may, therefore, play a role in the patterning of the nervous circuitry.

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Influence of glycosaminoglycans on neurite morphology and outgrowth patterns in vitro

Verna

Fichard

Saxod

1989

Intl J of Devlp Neuroscience

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Effects of tunicamycin on the avoidance reaction of epidermis by sensory neurites in co‐cultures

Fichard

Verna

Saxod

1990

Intl J of Devlp Neuroscience

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In 7-day chick embryo dorsal root ganglia and epidermis or dermis co-cultures, nerve fibres establish contacts with dermis while avoiding epidermis. Previous results have indicated that factor(s) secreted by epidermis could be involved in this avoidance reaction. The present study demonstrates that the avoidance reaction is abolished when epidermal cells are treated by the N-linked glycoproteins synthesis inhibitor, tunicamycin. The same result is obtained after monensin treatment. The epidermal cell viability, development and total protein secretion are not significantly affected by tunicamycin, as demonstrated by trypan blue exclusion, electron microscopy and SDS-PAGE electrophoresis after 35S-methionine labelling. It has thus been concluded that the avoidance factor is glycoproteic in nature. It is also suggested that this factor possibly contains chondroitin-6-sulphate moieties.

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Cohesion and Desquamation of Epidermal Stratum Corneum

Brysk

Rajaraman

1992

Progress in Histochemistry and Cytochemistry

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Glycoconjugate biosynthesis in human epidermis

Cited by 3 publications

References 21 publications

Influence of glycosaminoglycans on neurite morphology and outgrowth patterns in vitro

Influence of glycosaminoglycans on neurite morphology and outgrowth patterns in vitro

Effects of tunicamycin on the avoidance reaction of epidermis by sensory neurites in co‐cultures

Cohesion and Desquamation of Epidermal Stratum Corneum

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