Studies on the Osmotic Fragility of Erythrocytes Influenced by a Metabolic Acidosis

Hiro, Takumitsu

doi:10.7600/jspfsm1949.31.279

Cited by 12 publications

(12 citation statements)

References 10 publications

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“…As one factor of intravascular hemolysis, it has been reported that the level of serum lactic acid is increased by exercise, the blood is acidified, and the erythrocyte membrane resistance is consequently decreased 12. One of the causes of hemolysis shown in this study may be that the level of blood lactic acid after the intense exercise was increased.…”

Section: Discussionmentioning

confidence: 48%

“…Various studies have been performed on the factors involved in hemolysis and exceeding the normal exercise tolerance, and it was reported that intravascular hemolysis may develop due to physical factors, such as the bursting of red blood cells in the circulation due to the impact of footfalls,8,9 or an increase in friction between red blood cells and vessel walls due to increased blood flow 10,11. Furthermore, several studies have indicated that erythrocyte membrane compromise due to factors such as lactic acid, lysolecithin, or oxidative stress, which increase in blood when the normal exercise tolerance is exceeded, could also be involved in hemolysis 12–17. Therefore, the development of exercise-induced hemolysis has been attributed to not only physical, but also chemical factors.…”

Section: Introductionmentioning

confidence: 99%

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Intense Exercise Increases Protein Oxidation in Spleen and Liver of Mice

Kobayashi

Nakatsuji

Aoi

et al. 2014

Nutr Metab Insights

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Studies have indicated that sports anemia is mainly associated with intravascular hemolysis induced by exercise. We hypothesized that such exercise-induced hemolysis leads to oxidative damage due to an increase in free iron caused by hematocyte destruction. Thirty-one male ICR mice were randomly divided into 3 groups: a rested control group, an intense-exercise group, and a group rested for 24 hours after intense exercise. The serum haptoglobin level of the intense-exercise group decreased compared with that of the rested control group, suggesting hemolysis. Tissue iron and protein carbonyl levels in the liver were increased after exercise, and the protein carbonyl level in the spleen on the day after exercise was significantly increased compared with that of the resting state. These results suggest that the spleen and liver, where extravascular hemolysis occurs, were subjected to oxidative modification by the free iron, which was released from large numbers of hemocytes that were destroyed due to the intense exercise.

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Section: Discussionmentioning

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Intense Exercise Increases Protein Oxidation in Spleen and Liver of Mice

Kobayashi

Nakatsuji

Aoi

et al. 2014

Nutr Metab Insights

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“…The acceleration of erythrocyte osmotic fragility during exercise was evaluated for the cause of sports anemia [16]. It was reported that the osmotic fragilities of human and/or dog red cells was accelerated by physical stress: friction between the red cell membrane and blood vessels [3,4], and chemical stresses: the release of lysolecithin, which can injure the erythrocytes, from the spleen by the increased secretion of epinephrine [14], and the increase of blood lactate caused by metabolic acidosis [4,7] during exercise.…”

mentioning

confidence: 99%

Effects of Splenic Erythrocytes and Blood Lactate Levels on Osmotic Fragility of Circulating Red Cells in Thoroughbred Horses during Exercise.

Hanzawa

Kubo

Kai

et al. 1998

JES

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Changes in the packed cell volume (PCV), blood lactate concentration [La-] and osmotic fragility of circulating erythrocytes were determined in three horses subjected to treadmill exercises or treated with epinephrine. The osmotic fragility was taken as the haemolysis rate (HL) in 0.56% NaCl. Previous warm-up significantly decreased HL by 24%, and cantering at 8 m/ s for 8 min (10% incline) increased HL by 92%; subsequent cool-down significantly decreased the rate by 23% to the initial resting level, but thereafter the rate gradually increased again, returning to the initial resting level. In contrast, continuous trotting at 5 m/s for 15 min (0% incline) decreased the HL by 50% to the initial resting level. Intravenous injection of epinephrine had little effect on the HL. Although the exercises in combination with the injection significantly increased PCV and [La-], these changes in the experiments were not correlated with the HL, but the correlation coefficient for PCV and [La-] and HL during cantering was positive: r=0.875 and 0.958, P<0.01, respectively. These results suggest that: 1) the release of red cells from the spleen into the circulation has no effect on changes in the osmotic fragility of erythrocytes during exercise; 2) heavy exercise increases the osmotic fragility, but 3) light exercise decreases it.

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“…In this study, MCHC was decreased, and both osmotic fragility and plasma hemo globine concentration were increased by exercise. These phenomena suggested that exercise produces promotion of fragility of red cell membrane and hemolysis, resulted in the release of hemoglobin from red cells to plasma [3,6,8].…”

Section: Discussionmentioning

confidence: 99%

“…The increase of plasma K+ is caused by releasing of the K+ from skeletal muscle due to the inhibition of Nat, K+-ATPase activity on the muscular cell membrane by production of lactate and deficiency of ATP by exercise [10]. However, generally, physical-and chemical stresses during heavy exercise promote fragility of red cell membrane and hemo lysis [6][7][8], resulted in the release of intra cellular substances from red cells to plasma [l, 8,21].…”

mentioning

confidence: 99%