Studies on the Mass Culture of Various Algae in Carboys and Deep-Tank Fermentations

Pruess, L. M.; Arnow, Peter; Wolcott, Lawrence; Bohonos, N.; Oleson, J. J.; Williams, J. H.

doi:10.1128/am.2.3.125-130.1954

Cited by 5 publications

References 7 publications

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A method of achieving photosynthesis in a stainless steel fermentor

Hedén

Levin

1959

Biotechnol. Bioeng.

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,'iummury. A description of the cultivation of Rhodospirillum rubrum in 1,000 and 3,000 1. fermentors fitted with a special illnmination device, and a discussion of the results. IntroductionCurrent interest in microbial photosynthesis frequently causes a need for large quantities of cell material which can be used for chemical studies of the process. Many special devices have been described, particularly for mass culture of algae (Myers et aE.;1.6 Burlew;2 Gummert, Meffert and Stratmann;3 Tamiya et aZ.;4,798Krauss and Thomas;5 Kanazawa, Fujita, Yuhara and Sasa;g Enebo;lO Fogg, Smith and Millerll), but to our knowledge only Preuss et a1.12 have used ordinary closed fermentors for photosynthesis work. They illuminated the culture through a window on the tank, a method which, however, has some drawbacks. The purpose of the present paper is to describe the use of a lamp inside a fermentor where the culture is given a large surface in a vortex formed by a bottom stirrer (cf. Chain et a1.13). The technique has been worked out for Rhodospirillum rubrum, but we feel that the ease with which an ordinary fermentor is adapted to photosynthesis might provide useful knowledge also for those working with other types of microbial photosynthesis. Background to the Experiments on R . rubrum

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A method of achieving photosynthesis in a stainless steel fermentor

Hedén

Levin

1959

Biotechnol. Bioeng.

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Heterotrophic Culture of Algae

Zajic

Chiu

1970

Properties and Products of Algae

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Fatty acids in green algae cultivated on a pilot-plant scale

Podojil

Lívanský²,

Prokeš³

et al. 1978

Folia Microbiol

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Fatty acids from Chlorella vulgaris, Scenedesmus obliquus var. acutus and from a mixed culture of the two strains, Melnik, were converted to methyl esters, separated by gas chromatography, and identified by means of standards. The spectrum of fatty acids included both saturated and unsaturated acids (with odd and even numbers of carbon atoms) from C12 to C22. Fatty acids C16:0, C18:0 and C20:3 were the major components in all cultures. Pure strains differed from the mixed culture in the production of C18:1, C12:0 and C19:2 acids; the first of these was present in higher amounts in pure cultures only, the latter two being found in the mixed culture. The level of lipids was lower as compared to the literature data and their extractability was affected by the manner of preparation of algae and extraction conditions.

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Studies on the Mass Culture of Various Algae in Carboys and Deep-Tank Fermentations

Cited by 5 publications

References 7 publications

A method of achieving photosynthesis in a stainless steel fermentor

A method of achieving photosynthesis in a stainless steel fermentor

Heterotrophic Culture of Algae

Fatty acids in green algae cultivated on a pilot-plant scale

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