Studies on the Interaction of Organic Phosphates with Haemoglobin in an Amphibian (Bufo marinus), a Reptile (Trachydosaurus rugosus) and Man

Coates, MichaeI

doi:10.1071/bi9750367

Aust. Jnl. Of Bio. Sci.

1975

DOI: 10.1071/bi9750367

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Studies on the Interaction of Organic Phosphates with Haemoglobin in an Amphibian (Bufo marinus), a Reptile (Trachydosaurus rugosus) and Man

MichaeI Coates¹

Abstract: The primary organic phosphate modifiers of haemoglobin function are DPG (2,3-diphosphoglycerate) in the toad Bufo marinus and ATP in the lizard Trachydosaurus rugosus. Myo-IP6 (myoinositol hexaphosphate) and myo-IPs (myo-inositol pentaphosphate) are more effective than ATP or DPG in reducing the oxygen affinities of the haemoglobins of B. marinus, T. rugosus and man, while ATP and DPG are about equally effective. Competition experiments indicate that ATP, DPG and myo-IP6 bind to the same site or sites on the h… Show more

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Cited by 12 publications

References 17 publications

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High levels of inosine monophosphate in the erythrocytes of elasmobranchs

1978

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The acid soluble organic phosphates of the erythrocytes of three species of elasmobranchs were assayed by chromatography on Dowex 1 anion exchange columns. Organic phosphates in the peaks eluted from these columns were identified by their ultraviolet absorption spectra and by further chromatography on paper. All three species are unusual amongst the vertebrates in that their erythrocytes contain high levels of inosine monophosphate (IMP). IMP has little effect on the oxygen affinity of the hemoglobins of the two species tested.

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High levels of inosine monophosphate in the erythrocytes of elasmobranchs

1978

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Ontogenetic changes in erythrocytic organic phosphates in the bullfrog, Rana catesbeiana

Hazard

Hutchison

1978

J. Exp. Zool.

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The metamorphosis of anuran tadpoles includes adjustments to different respiratory media and involves changes in all components of the respiratory system. Increased concentrations of erythrocytic organic phosphates decrease blood oxygen affinity in vertebrates. Nucleoside triphosphates (NTP) , 2,3 diphosphoglycerate (DPG), inositol polyphosphate (I-poly-P) and total phosphate were measured in different stages of tadpoles, juveniles and adults. The concentrations of NTP, DPG, and I-poly-P were, respectively: 4.35, 1.90 and 1.12 pmol ml-' RBC in tadpoles, and 1.80, 1.10 and 0.393 pmol ml-' RBC in juveniles and adults. Molar ratios also decreased a t metamorphosis: NTP/Hb from 1.83 to 0.49, DPG/Hb from 0.814 to 0.296, and I-poly-P/Hb from 0.483 to 0.061. The decrease in NTP occurs simultaneously with the transition from tadpole to adult hemoglobin type. The decrease in DPG parallels the decline in blood pH during metamorphosic climax. Levels of I-poly-P were not correlated with developmental changes of the respiratory system. The developmental stages of the decrease in red cell organic phosphate concentrations coincide with the suite of adaptive adjustments which lead to aerial respiration in metamorphosing bullfrogs.Krogh and Leitch ('19) first proposed that vertebrate erythrocytes might contain compounds, other than hemoglobin, which could modulate whole blood respiratory function. Greenwald ('25) reported large quantities of 2,3 diphosphoglycerate (DPG) in porcine red cells, and in a more comprehensive study, Rapoport and Guest ('41) found DPG, adenosine triphosphate (ATP), and phytic acid (inositol hexaphosphate, IHP) in red cells of several vertebrate species. A connection between such organic phosphates and respiratory function was established by Benesch and Benesch ('67), Benesch et al. ('68) and Chanutin and Curnish ('67). These workers demonstrated that DPG was an allosteric ligand for hemoglobin which increased the Ps0, decreased the oxygen affinity and enhanced tissue oxygen delivery. Bartlett ('70) established broad phylogenetic distribution patterns for organic phosphates. In fishes, ATP (Gilleen and Riggs, '711, guanosine triphosphate (GTP) (Geoghegan and Poluhowich, '74) or inositol pentaphosphate (Isaacks et al., '77) have been reported as major erythrocytic phosphates. In birds, the IHP originally reported by Rapoport and later by

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Isolation of O‐phosphorylethanolamine from the erythrocytes of an elasmobranch (the Southern Fiddler Ray — Trygonorhina fasciata guanerius)

Coates¹,

Thompson²,

Tate³

1979

J. Exp. Zool.

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An amphoteric organic phosphate, which represents 20% of erythrocyte acid soluble phosphate components from an elasmobranch (the Southern Fiddler Ray — Trygonorhina fasciata guanerius) has been isolated as a colourless crystalline solid. Its phosphorus content, melting point behaviour, chromatographic, electrophoretic and ion‐exchange properties, together with its infrared and electron impact mass spectra are consistent with its formulation as O‐phosphorylethanolamine. Assuming minimal degradation in the isolation procedure, the calculated erythrocyte intracellular concentrations of characterised phosphates from the Southern Fiddler Ray are as follows: O‐phosphorylethanolamine (1.89 mM), adenosine triphosphate (1.61 mM), inorganic phosphate (0.26 mM), inosine monophosphate (0.47 mM), guanosine triphosphate (0.34 mM) and adenosine diphosphate (0.11 mM). On a molar basis O‐phosphorylethanolamine is the major acid soluble phosphate of the Southern Fiddler Ray erythrocyte, but examination of the literature suggests its concentration may vary considerably among other elasmobranchs and possible reasons for such variance are considered.

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Studies on the Interaction of Organic Phosphates with Haemoglobin in an Amphibian (Bufo marinus), a Reptile (Trachydosaurus rugosus) and Man

Cited by 12 publications

References 17 publications

High levels of inosine monophosphate in the erythrocytes of elasmobranchs

High levels of inosine monophosphate in the erythrocytes of elasmobranchs

Ontogenetic changes in erythrocytic organic phosphates in the bullfrog, Rana catesbeiana

Isolation of O‐phosphorylethanolamine from the erythrocytes of an elasmobranch (the Southern Fiddler Ray — Trygonorhina fasciata guanerius)

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