Studies on the interaction between nanodiamond and human hemoglobin by surface tension measurement and spectroscopy methods

Pishkar, Leila; Taheri, Saba; Makarem, Somayeh; Zeinabad, Hojjat Alizadeh; Rahimi, Arash; Saboury, Ali Akbar; Falahati, Mojtaba

doi:10.1080/07391102.2016.1155172

Cited by 37 publications

(16 citation statements)

References 41 publications

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“…In general, intermolecular interacting forces between a ligand and a protein include hydrogen bonding, van der Waals interaction, electrostatic forces, and hydrophobic network. 43 The thermodynamic parameters such as enthalpy change (ΔH°), standard entropy change (ΔS°), and standard Gibbs free energy change (ΔG°) of interaction were determined using van't Hoff equation (Equation 3): 43…”

Section: Thermodynamic Parametersmentioning

confidence: 99%

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Anbouhi¹,

Esfidvajani²,

Nemati³

et al. 2018

IJN

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BackgroundNanoparticles (NPs) have been emerging as potential players in modern medicine with clinical applications ranging from therapeutic purposes to antimicrobial agents. However, before applications in medical agents, some in vitro studies should be done to explore their biological responses.AimIn this study, protein binding, anticancer and antibacterial activates of zero valent iron (ZVFe) were explored.Materials and methodsZVFe nanoparticles were synthesized and fully characterized by X-ray diffraction, field-emission scanning electron microscope, and dynamic light scattering analyses. Afterward, the interaction of ZVFe NPs with human serum albumin (HSA) was examined using a range of techniques including intrinsic fluorescence, circular dichroism, and UV–visible spectroscopic methods. Molecular docking study was run to determine the kind of interaction between ZVFe NPs and HSA. The anticancer influence of ZVFe NPs on SH-SY5Y was examined by MTT and flow cytometry analysis, whereas human white blood cells were used as the control cell. Also, the antibacterial effect of ZVFe NPs was examined on Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), and Staphylococcus aureus (ATCC 25923).ResultsX-ray diffraction, transmission electron microscope, and dynamic light scattering analyses verified the synthesis of ZVFe NPs in a nanosized diameter. Fluorescence spectroscopy analysis showed that ZVFe NPs spontaneously formed a complex with HSA through hydrogen bonds and van der Waals interactions. Also, circular dichroism spectroscopy study revealed that ZVFe NPs did not change the secondary structure of HSA. Moreover, UV–visible data presented that melting temperature (Tm) of HSA in the absence and presence of ZVFe NPs was almost identical. Molecular dynamic study also showed that ZVFe NP came into contact with polar residues on the surface of HSA molecule. Cellular assays showed that ZVFe NPs can induce cell mortality in a dose-dependent manner against SH-SY5Y cells, whereas these NPs did not trigger significant cell mortality against normal white bloods in the concentration range studied (1–100 µg/mL). Antibacterial assays showed a noteworthy inhibition on both bacterial strains.ConclusionIn conclusion, it was revealed that ZVFe NPs did not induce a substantial influence on the structure of protein and cytotoxicity against normal cell, whereas they derived significant anticancer and antibacterial effects.

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Section: Thermodynamic Parametersmentioning

confidence: 99%

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Anbouhi¹,

Esfidvajani²,

Nemati³

et al. 2018

IJN

Self Cite

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“…41 The interaction of nanodiamond and silicon dioxide NPs with Hb also showed secondary and tertiary structural changes of Hb. 42,43 It has been shown that fullerene NPs synthesized by different methods induce various impacts on human serum albumin and bovine serum albumin conformations. The crucial difference between the two investigations was the fabrication methods of fullerene NPs, and principal differences between the main NPs fabricated by the two methods were the NP size distributions.…”

Section: Discussionmentioning

confidence: 99%

Biophysical, bioinformatical, cellular, and molecular investigations on the effects of graphene oxide nanosheets on the hemoglobin structure and lymphocyte cell cytotoxicity

Babadaei¹,

Moghaddam²,

Solhvand³

et al. 2018

IJN

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BackgroundImplementations of nanoparticles have been receiving great interest in medicine and technology due to their unique characteristics. However, their toxic impacts on the biological system are not well explored.AimThis study aims to investigate the influence of fabricated nano graphene oxide (NGO) sheets on the secondary and quaternary structural alterations of human hemoglobin (Hb) and cytotoxicity against lymphocyte cells.Materials and methodsDifferent spectroscopic methods, such as extrinsic and synchronous fluorescence spectroscopy and far circular dichroism (CD) spectroscopy, molecular docking investigation, cellular assays (trypan blue exclusion, cellular uptake, ROS, cell cycle, and apoptosis), and molecular assay (fold changes in anti/proapoptotic genes [B-cell lymphoma-2 {BCL2}/BAX] expression levels) were used in this study.ResultsTransmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and zeta potential investigations revealed the nano-sized nature of NGOs with good colloidal stability. Extrinsic fluorescence spectroscopy by using 8-anilinonaphthalene-1 -sulfonic acid and synchronous fluorescence spectroscopy showed that NGOs can unfold the quaternary structure of Hb in the vicinity of Tyr residues. The CD investigation demonstrated that the α-helicity of Hb experienced substantial alteration upon interaction with increasing concentrations of NGOs. The molecular docking study showed that NGOs interacted with polar residues of Hb. Cellular and molecular assays revealed that NGOs lead to ROS formation, cell cycle arrest, and apoptosis through the BAX and BCL2 pathway.ConclusionThese data reveal that NGOs can induce some protein structural changes and stimulate cytotoxicity against normal cell targets. Therefore, their applications in healthy systems should be limited.

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“…A number of techniques such as nuclear magnetic resonance (NMR) spectroscopy [78], X-ray crystallography [79], circular dichroism spectroscopy [80], isothermal calorimetry [81], differential scanning calorimetry [82], fluorescence spectroscopy [83], and UV-visible spectroscopy [84] have been widely used for analyzing the protein-NP interactions. The NPinduced conformational changes and subsequent corona formation depends on several factors such as, protein type, NP type, size of NP, shape of NP, pH and the temperature.…”

Section: The Effect Of Np On the Protein Conformational Changesmentioning

confidence: 99%

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

Ajdari¹,

Moosavi²,

Rahmati³

et al. 2018

Preprint

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Nanoparticles (NPs) are widely used in diverse disciplines, including biology, medicine science. The central question that need to be answered is whether NPs have toxic effects on biological cells and molecules or are they safe. The safety of NPs including targeted drug delivery is critical and so is their toxicity in the environment. In recent years, in vitro and in vivo research on animals has generated abundant information about the toxicity of NPs.However, due to varying laboratory conditions, the comparison of the results from ensuing studies is somewhat unreliable. It should be noted that, depending on the type of production, NPs can enter the body through inhalation, skin and via digestive routes. Due to the diversity of NPs and their properties, there is paucity of accurate information on their toxicological effects; particle size, shape, surface area and the chemical levels are considered as key factors in creating health and toxicological effects. Consequently, there is a need for reliable information about their effects on various organs so as to deal with NPs effectively and their impact on health and the environment. This review covers the existing knowledge base on the subject that hopefully prepares us better to address these challenges.

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Studies on the interaction between nanodiamond and human hemoglobin by surface tension measurement and spectroscopy methods

Cited by 37 publications

References 41 publications

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Biophysical, bioinformatical, cellular, and molecular investigations on the effects of graphene oxide nanosheets on the hemoglobin structure and lymphocyte cell cytotoxicity

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

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