Studies on the in vitro genotoxicity of baytubes®, agglomerates of engineered multi-walled carbon-nanotubes (MWCNT)

Potential for exposure to engineered nanoparticles from nanotechnology-based consumer spray products

“…The detected large particles are likely to carry material from product matrix and agglomerates of nano-sized particles. Due to the importance of agglomerates in examining biological effects (Wick et al, 2007; Qu et al, 2009; Wirnitzer et al, 2009), subsequent research will need to examine the internal structure and composition of the released super-micron particles including the stability of the nanomaterials in agglomerates at the site of deposition in the lung. Also, testing the effect of spray cone orientation within the breathing zone will be a subject of future studies.…”

In vivo genotoxicity study of single-wall carbon nanotubes using comet assay following intratracheal instillation in rats

“…There is little information about the possible human health and environmental impacts of manufactured CNTs. Early studies suggested that SWCNTs did not have mutagenic effects in Salmonella typhimurium YG1024 and YG1029 strains , and MWCNTs did not have mutagenic effects in the bacterial reverse mutation assay (Di Sotto et al, 2009;Wirnitzer et al, 2009). In an in vitro comet assay (single cell gel electrophoresis), a mixture of SWCNTs and other CNTs increased the extent of DNA damage in human bronchial epithelial cells in a dose-dependent manner (Lindberg et al, 2009).…”

Quasi-SMILES and nano-QFAR: United model for mutagenicity of fullerene and MWCNT under different conditions

“…The numerical data on the mutagenicity (TA100) taken in the literature: for fullerene (Shinohara et al, 2009) and for multi-walled carbon-nanotubes (Wirnitzer et al, 2009). The numerical data on TA100 are converted into negative decimal logarithmic scale (pTA100): these values are examined as the endpoint for the QFAR analysis.…”