Studies on the Group F Antigen of Lactobacilli: Antigenicity and Serological Specificity of Teichoic Acid Preparations

Studies on the Group F Antigen of Lactobacilli: Detection of Antibodies by Haemagglutination

“…Cell wall did not absorb antibodies nor did the cell-wall polysaccharide react with antibodies to purified teichoic acid ( Knox et al 1970). It may, therefore, be concluded that absorption of antibodies implies their penetration of the cell wall to react with membrane teichoic acid.…”

“…of solution examined for reactivity with antiserum 148 (0.1 nil.) by the quantitative precipitin method (Heidelberger & MacPherson, 1943;Knox et al 1970). By comparison with P-teichoic acid standards, the teichoic acid content of the extracts was estimated.…”

“…The serological classification of group F lactobacilli has been shown to depend on a glycerol teichoic acid associated with the cell membrane and having glucose and galactose substituents : by using a mild phenol extraction procedure the isolated product contained both glycolipid and phospholipid, and retained its antigenicity (Knox, Hewett & Wicken, 1970;.…”

Serological Properties of the Wall and Membrane Teichoic Acids from Lactobacillus helveticus NCIB 8025

“…The specificity of group antisera was examined initially by the qualitative ring precipitin method (Sharpe, 1955). Quantitative precipitin reactions and haemagglutination titres were determined by the methods previously used with Lactobacillus fermenti Knox et al 1970).…”

Isolation of Lipoteichoic Acids from Butyrivibrio fibrisolvens

“…Chemical and serological methods. All chemical, chromatographic, analytical and serological procedures were as detailed in previous publications (Knox, Hewett & Wicken, 1970;Knox & Wicken, 1972;Wicken & Knox, 1971).…”