Serological Properties of the Wall and Membrane Teichoic Acids from Lactobacillus helveticus NCIB 8025

Knox, K. W.; Wicken, A. J.

doi:10.1099/00221287-63-2-237

Cited by 42 publications

(31 citation statements)

References 20 publications

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“…Streptococci are known to contain glycerolphosphate teichoic acid polymers (6,12,13,24) that are associated with the cytoplasmic membrane (8,18,20). A similar location has recently been shown in the lactobacilli (9). A large part of the immunological specificity of these polymers is due to the glycerophosphate "backbone" (9,12), and as such is responsible for the serological cross-reactions among many grampositive bacteria.…”

Section: Downloaded Fromsupporting

confidence: 65%

Extraction, Purification, and Chemical and Immunological Properties of the Streptococcus mutans Group “a” Polysaccharide Cell Wall Antigen

Mukasa

Slade

1973

Infect Immun

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An antigen of Streptococcus mutans has been extracted from HS6 (group "a") whole cells and repeatedly fractionated by Sephadex chromatography. The antigen is shown to be a polysaccharide and contains the S. mutans group "a" antigenic site and also a second antigenic site which is common to "a" strains and 2 of 3 group "d" strains. Immunological electrophoretic and chromatographic data indicate that the two sites exist in a single molecule. The polysaccharide has a molecular weight of 107,000 and is composed of glucose, galactose, glucosamine, and galactosamine. No significant quantities of lipid, phosphorus, glycerol, or ribitol are present. Immunological specificity of the group "a" polysaccharide site depends primarily on a D-glucose D-glucose sequence, the "a-d" site on a terminal D-galactose. Water at 100 C and pepsin (pH 2.5) at room temperature are very effective in extracting the polysaccharide from lyophilized S. mutans cells. Trypsin and lysozyme are less effective. The antigen-antibody combining site appears to be located at the cell wall surface. A small quantity of enzyme-resistant protein (5%) is firmly linked to the antigen and is considered to be a remnant of a protein to which the polysaccharide is attached in the cell wall. The composition of the protein does not identify it as a part of the peptidoglycan. No reaction to the purified polysaccharide is obtained with antisera specific for teichoic acid glycerophosphate polymers from streptococci, staphylococci, or lactobacilli. "a-d" antigen. 190 on July 31, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Downloaded Fromsupporting

confidence: 65%

Extraction, Purification, and Chemical and Immunological Properties of the Streptococcus mutans Group “a” Polysaccharide Cell Wall Antigen

Mukasa

Slade

1973

Infect Immun

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“…Assuming a composition similar to that reported for another L. helveticus strain (Knox & Wicken, 1971), the teichoic acid content was estimated at about 14% for isolated cell walls and 21% for SDS-treated cell walls.…”

Section: Composition Of Isolated Cell Wallsmentioning

confidence: 61%

“…Previous studies also revealed the presence of glyceroltype teichoic acids (Baddiley & Davison, 1961) and neutral sugars (glucose, galactose but no rhamnose). Wall and membrane teichoic acids of L. helveticus were shown to have antigenic properties (Knox & Wicken, 1971). It has also been suggested that the cell wall of L. helveticus ATCC 10797 was composed of two layers with the outer layer presumably containing a predominant protein (Masuda & Kawata, 1983).…”

Section: Introductionmentioning

confidence: 99%

Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046

Lortal¹,

Rousseau

Boyaval³

et al. 1991

Journal of General Microbiology

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The cell wall of Lactobacillus helveticus was found to have a three-layered structure when thin sections of whole cells or isolated cell walls were stained by various procedures and examined by electron microscopy. The main feature of the composition of isolated cell walls was a high protein content (about 48% of the cell wall dry weight) with a predominant 52 kDa protein. Peptidoglycan and what was considered as teichoic acids accounted for 36% and 14%, respectively, of the cell wall dry weight. The 52 kDa protein was apparently unglycosylated, located in the outer layer and noncovalently bound to the inner layers. Peptidoglycan was mostly assigned to the innermost layer. The conditions leading to autolysis of whole cells or isolated cell walls were determined, as well as the specificity of the autolysin involved in cell wall degradation. IntroductionLactobacillus helveticus is an important lactic acid bacterium that is involved in cheese technology (Accolas et al., 1980;Turner et al., 1983) and that has recently become the object of interest for genetic manipulation (Chassy, 1987;Sandine, 1987;Thomas & Collins, 1988). Its autolysis with the concomitant release of cytoplasmic proteases (Ezzat et al., 1986) is an essential feature of cheese maturation (Bie & Sjostrom, 1975a, b). A better understanding of the autolytic system of L. helveticus could perhaps offer a new way for an accelerated ripening of cheese. Ohmiya & Sat0 (1970) were the first to follow the autolysis of L. helveticus in technological conditions. After adding whole cells to an aseptic rennet curd, the release of DNA was noted and autolysed cells were clearly observed by electron microscopy. More recently, we promoted the autolysis of L. helveticus by suspending whole cells in dilute buffers (Lortal et al., 1989). A clear understanding of the autolytic system of L. helveticus implies, in particular, a better knowledge of the composition and structure of its cell wall, which have so far been investigated only to a limited extent (Ikawa & Snell, 1960;Baddiley & Davison, 1961 ; Williams, 1971). The main peptidoglycan amino acids were determined (Glu, Ala, Asp, Lys) as well as their respective percentage in the D-configuration (Ikawa & Snell, 1960). Previous studies also revealed the presence of glyceroltype teichoic acids (Baddiley & Davison, 1961) and neutral sugars (glucose, galactose but no rhamnose). Wall and membrane teichoic acids of L. helveticus were shown to have antigenic properties (Knox & Wicken, 1971). It has also been suggested that the cell wall of L. helveticus ATCC 10797 was composed of two layers with the outer layer presumably containing a predominant protein (Masuda & Kawata, 1983).In the work described here, a more detailed investigation of the morphology and composition of the cell wall of L. helveticus ATCC 12046 was undertaken. Moreover, the main characteristics of the autolytic system of this strain were investigated, in particular ways of promoting lysis, the effect of external factors, and the specificity of the autolytic...

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“…It is conceivable that the structural diversity seen in WTA is also present in other lactic acid bacteria, but little is known about the structures of these other species. 27,28) Minimal knowledge of the WTAs of Lactobacillus buchneri and L. helveticus has been obtained by chemical decomposition methods. Although the WTA of L. plantarum has been the most frequently studied among the WTAs of lactic acid bacteria, the monomeric structures of glycerol-type WTA have not yet been determined.…”

Section: Discussionmentioning

confidence: 99%

Structures of Two Monomeric Units of Teichoic Acid Prepared from the Cell Wall ofLactobacillus plantarumNRIC 1068

Tomita¹,

Furihata²,

Nukada³

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Serological Properties of the Wall and Membrane Teichoic Acids from Lactobacillus helveticus NCIB 8025

Cited by 42 publications

References 20 publications

Extraction, Purification, and Chemical and Immunological Properties of the Streptococcus mutans Group “a” Polysaccharide Cell Wall Antigen

Extraction, Purification, and Chemical and Immunological Properties of the Streptococcus mutans Group “a” Polysaccharide Cell Wall Antigen

Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046

Structures of Two Monomeric Units of Teichoic Acid Prepared from the Cell Wall ofLactobacillus plantarumNRIC 1068

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