Studies on the generative nuclear division. III. Effect of exogenous ATP on the generative nuclear division in Lilium longiflorum.

Kamizyô, Akio; Tanaka, Norihiro

doi:10.1508/cytologia.47.195

Cited by 9 publications

(3 citation statements)

References 6 publications

(6 reference statements)

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“…Early studies demonstrated that exogenous application of ATP could modulate stomatal aperture in Commelina communis and the stimulation of pollen tube generative nuclear divisions in Lilium longiflorum (Kamizyo and Tanaka, 1982;Nejidat et al, 1983). Recent results show that micromolar levels of exogenous ADP increase the growth rate of Arabidopsis thaliana root hairs (Lew and Dearnaley, 2000), and millimolar concentrations of xATP suppress the gravitropic growth of Arabidopsis roots (Tang et al, 2003) as well as inhibit Arabidopsis pollen germination (Steinebrunner et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Evidence of a Novel Cell Signaling Role for Extracellular Adenosine Triphosphates and Diphosphates in Arabidopsis

et al. 2004

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Extracellular ATP is a known receptor agonist in animals and was previously shown to alter plant growth, and so we investigated whether ATP derivatives could function outside plant cells as signaling agents. Signaling responses induced by exogenous nucleotides in animal cells typically include increases in free cytoplasmic calcium concentration ([Ca 2þ ] cyt ). We have evaluated the ability of exogenously applied adenosine 59-[g-thio]triphosphate (ATPgS), adenosine 59-[b-thio]diphosphate (ADPbS), and adenosine 59-O-thiomonophosphate to alter [Ca 2þ ] cyt in intact apoaequorin transgenic Arabidopsis thaliana seedlings. ATPgS and ADPbS increase [Ca 2þ ] cyt , and this increase is enhanced further when the nucleotides are added with the elicitor oligogalacturonic acid. Exogenous treatment with ATP also increases the level of transcripts encoding mitogen-activated protein kinases and proteins involved in ethylene biosynthesis and signal transduction. The increase in [Ca 2þ ] cyt induced by nucleotide derivatives can be ablated by Ca 2þ -channel blocking agents and by the calcium chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N9,N9-tetraacetic acid (BAPTA), and the changes in gene expression can be partially blocked by these agents. These observations suggest that extracellular ATP can activate calcium-mediated cell-signaling pathways in plants, potentially playing a physiological role in transducing stress and wound responses.

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Section: Introductionmentioning

confidence: 99%

Evidence of a Novel Cell Signaling Role for Extracellular Adenosine Triphosphates and Diphosphates in Arabidopsis

et al. 2004

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“…Early observations of nucleotide effects on plant biological processes suggest that extracellular ATP could be a signaling agent in plants as well. For example, these reports show that exogenous application of ATP increases Lilly pollen tube mitotic activity [ 1 ] modulates stomatal guard cell aperture [ 2 ], and promotes the closure of the Venus fly trap [ 3 ]. Externally applied caffeine, another purine derivative, induces pronounced inhibitory effects on plant developmental and cellular processes, including sieve plate formation [ 4 ], tracheary element differentiation [ 5 ] and pollen tube growth [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Plant responses to extracellular nucleotides: Cellular processes and biological effects

Jeter

Roux

2006

Purinergic Signalling

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Higher plants exhibit cellular responsiveness to the exogenous application of purine nucleotides in a manner consistent with a cellYcell signaling function for these molecules. Like animals, plants respond to extracellular ATP, ADP, and stable analogues (e.g., ATPgS and ADPbS) by increasing the cytoplasmic concentration of calcium. Agonist substrate specificity and concentration dependency suggest receptor mediation of these events, and, although the identity of the plant receptor is currently unknown, pharmacological analysis points to the involvement of a plasma membrane-localized calcium channel. Extracellular ATP can also induce the production of reactive oxygen species and stimulate an increase in the mRNA levels of a number of stress-and calcium-regulated genes, suggesting a role for nucleotide-based signaling in plant wound and defense responses. Furthermore, the growth and development of plants can also be altered by the application of external ATP. Recent studies are only beginning to uncover the complexities of plant signaling networks activated in response to extracellular ATP and how these might interact to affect plant physiological processes.

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“…In our previous papers (Kamizyo andTanaka 1982a, 1982b), it has been reported that progressive rate of mitosis of the pollen tube generative nucleus varied according to the difference in culture medium. Namely, in Lilium longiflorum, the generative nuclear division was greatly depressed in the artificial medium than in the stigmatic exudate medium.…”

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confidence: 94%

Studies on the generative nuclear division. V. Effect of ageing of pollen grains on the generative nuclear division in Tradescantia paludosa.

Kamizyô

Tanaka

1982

CYTOLOGIA

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In our previous papers (Kamizyo and Tanaka 1982a, 1982b), it has been reported that progressive rate of mitosis of the pollen tube generative nucleus varied according to the difference in culture medium. Namely, in Lilium longiflorum, the generative nuclear division was greatly depressed in the artificial medium than in the stigmatic exudate medium. But addition of a suitable concentration of exogenous ATP into the artificial medium has slightly improved the mitotic activity. And furthermore, in both plant species of Lilium longiflorum and Tradescantia paludosa, the mitosis was elated more satisfactorily in the medium supplemented with 10% sucrose than in the medium supplemented with 20% sucrose.It has been well known that artificial storage of pollen grains for a certain time results in erratic germination and destituted elongation of pollen tube in vitro (Johri and Vasil 1961, Linskens 1975). In the course study of pollen tube culture in Tradescantia paludosa, it has been noticed that the ageing of pollen grains affected both viability and mitotic activity of the generative nucleus. This problem will be described here. Materials and methodsThe materials used in this study were taken from the same source as described previously (Kamizyo and Tanaka 1982b), but the methods applied here were slightly modified as follows: A large number of pollen grains collected in the morning of the day of experiments, which will be referred to hereafter as "fresh pollen grains" was allowed to stand for about seven hours at room temperature, which will be referred to as "aged pollen grains". Thus aged pollen grains were sown on the artificial medium at 3p. m., and fixed at 9p. m. and 11p. m., which will be referred to according to the culture duration as "6 hr culture" and "8 hr culture", respectively. The culture medium was supplemented with sucrose of either concentration of 10 or 20%, which will be referred to as "10% sucrose medium" and "20% sucrose medium", respectively. The control cultures of fresh pollen grains were started at 8 a.m., and fixed at 2p. m. and 4p. m. in parallel. Taken procedure will be ab breviated similarly as described in our previous paper, i.e., an expression, "aged pollen grain-10% sucrose medium-6 hr culture", means that pollen grains were sown, after ageing, on the medium supplemented with 10% sucrose at 3p. m. and fixed at 9p. m., and so on.

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Studies on the generative nuclear division. III. Effect of exogenous ATP on the generative nuclear division in Lilium longiflorum.

Cited by 9 publications

References 6 publications

Evidence of a Novel Cell Signaling Role for Extracellular Adenosine Triphosphates and Diphosphates in Arabidopsis

Evidence of a Novel Cell Signaling Role for Extracellular Adenosine Triphosphates and Diphosphates in Arabidopsis

Plant responses to extracellular nucleotides: Cellular processes and biological effects

Studies on the generative nuclear division. V. Effect of ageing of pollen grains on the generative nuclear division in Tradescantia paludosa.

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