“…In recent years, sucrose-mineral solution so devised by Brewbaker and Kwack (1963) has been chiefly used for in vitro culture of pollen tubes and for following chromosome analysis (e.g., Kwack and Kim 1967, Tera saka and Tanaka 1974, Kamizyo and Tanaka 1978, 1981, in place of simple sucrose-agar plate (SAP) culture. However, many investigators have reported that, in most species, pollen tubes cultured in vitro failed to grow as long as enough to reach from stigma to egg cell in the embryo sac (e.g., Pfaher 1967, Rosen 1971, Stanley 1971.…”