Studies on the formation of bioconjugates of Endoglucanase with colloidal gold

Gole, Anand; Vyas, S. P.; Phadtare, Sangita; Lachke, Anil; Sastry, Murali

doi:10.1016/s0927-7765(01)00301-0

Cited by 57 publications

(39 citation statements)

References 57 publications

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“…Besides the common electrostatic interaction, some nanoparticles can also immobilize biomolecules by other interactions. For example, it is reported that gold nanoparticles can immobilize proteins through the covalent bonds formed between the gold atoms and the amine groups and cysteine residues of proteins [6,7].…”

Section: Immobilization Of Biomoleculesmentioning

confidence: 99%

Application of Nanoparticles in Electrochemical Sensors and Biosensors

et al. 2006

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The unique chemical and physical properties of nanoparticles make them extremely suitable for designing new and improved sensing devices, especially electrochemical sensors and biosensors. Many kinds of nanoparticles, such as metal, oxide and semiconductor nanoparticles have been used for constructing electrochemical sensors and biosensors, and these nanoparticles play different roles in different sensing systems. The important functions provided by nanoparticles include the immobilization of biomolecules, the catalysis of electrochemical reactions, the enhancement of electron transfer between electrode surfaces and proteins, labeling of biomolecules and even acting as reactant. This minireview addresses recent advances in nanoparticle-based electrochemical sensors and biosensors, and summarizes the main functions of nanoparticles in these sensor systems.

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Section: Immobilization Of Biomoleculesmentioning

confidence: 99%

Application of Nanoparticles in Electrochemical Sensors and Biosensors

et al. 2006

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“…The GNPs offer excellent candidates for the immobilization platform. The adsorption of biomolecules onto the surfaces of GNPs can retain their bioactivity and stability because of the biocompatibility and the high surface free energy of GNPs [76,77]. GNPs, as compared with flat gold surfaces, have a much higher surface area, allowing loading of a larger amount of protein and are potentially more sensitive.…”

Section: Gnps Act As Immobilization Platformmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

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“…From UV-Vis spectroscopy measurements, the amount of the F-prot bound to 10 mg of nanogoldzeolite was found to be 1.15 mg, with immobilization of the enzyme on the gold nanoparticles occurring via amine groups and cysteine residues in proteins that are known to bind strongly with gold colloids (Gole et al, 2001a(Gole et al, , 2001b(Gole et al, , 2002. Exposure of F-prot to 10 mg of amine-functionalized zeolite material resulted in 1.13-mg loading of the enzyme, presumably due to hydrogen bonding between the hydroxyl and amine groups of zeolite and carbonyl or amino groups in F-prot.…”

Section: Enzyme Quantitative Studiesmentioning

confidence: 99%

“…In this laboratory, some of us have reported on the bioconjugation of the enzymes pepsin (Gole et al, 2001a), fungal protease (F-prot) (Gole et al, 2001b), and endoglucanase (Gole et al, 2002) with gold nanoparticles. Although it was observed that the specific catalytic activity of the nanogold-enzyme bioconjugate material was comparable (and at times marginally in excess of) to that of the free enzyme in solution, and that the immobilized enzyme exhibited enhanced temperature and pH stability (Gole et al, 2001a(Gole et al, , 2001b(Gole et al, , 2002, the reuse characteristics of the bioconjugate material were poor.…”

Section: Introductionmentioning

confidence: 99%

“…Although it was observed that the specific catalytic activity of the nanogold-enzyme bioconjugate material was comparable (and at times marginally in excess of) to that of the free enzyme in solution, and that the immobilized enzyme exhibited enhanced temperature and pH stability (Gole et al, 2001a(Gole et al, , 2001b(Gole et al, , 2002, the reuse characteristics of the bioconjugate material were poor. Indeed, separation of the biocatalyst material from the reaction medium often could not be achieved, even by ultracentrifugation (Gole et al, 2001a(Gole et al, , 2001b(Gole et al, , 2002.This is clearly a major disadvantage in the use of otherwise biocompatible gold nanoparticles in the immobilization of enzymes. This problem may be overcome if the gold nanoparticles could be tethered at high density to a more massive surface, such as that provided by micron-sized particles.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization and biocatalytic activity of fungal protease on gold nanoparticle‐loaded zeolite microspheres

Phadtare

Vinod

Mukhopadhyay

et al. 2004

Biotech & Bioengineering

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Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. However, separation of the gold nanoparticle-enzyme bioconjugate material from the reaction medium is often difficult. In this study, we investigate the assembly of the gold nanoparticles on the surface of the amine-functionalized zeolite microspheres in the formation of zeolite-gold nanoparticle "core-shell" structures and, thereafter, the use of this structure in immobilization of fungal protease. The assembly of gold nanoparticles on the zeolite surface occurs through the amine groups present in 3-aminopropyltrimethoxysilane (3-APTS). The fungal proteases bound to the massive "core-shell" structures were easily separated from the reaction medium by mild centrifugation and exhibited excellent reuse characteristics. The biocatalytic activity of fungal protease in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability and a shift in the optimum temperature of operation.

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Studies on the formation of bioconjugates of Endoglucanase with colloidal gold

Cited by 57 publications

References 57 publications

Application of Nanoparticles in Electrochemical Sensors and Biosensors

Application of Nanoparticles in Electrochemical Sensors and Biosensors

Gold nanoparticle-based biosensors

Immobilization and biocatalytic activity of fungal protease on gold nanoparticle‐loaded zeolite microspheres

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