STUDIES ON THE FORMATION OF SPHEROPLASTS FROM STATIONARY PHASE CELLS OF<i>SACCHAROMYCES CEREVISIAE</i>

Russell, Inge; Garrison, I. F.; Stewart, Graham G.

doi:10.1002/j.2050-0416.1973.tb03499.x

Cited by 18 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The treatment of yeast cell walls with exogenous proteases causes the release of mannan (Russell et al, 1973). The possibility therefore exists that limited endoproteolysis of some mannoprotein molecules would detach peptide chains from the wall network.…”

Section: Discussionmentioning

confidence: 99%

Autolytic Release of Mannoproteins from Cell Walls of Saccharomyces cerevisiae

1985

View full text Add to dashboard Cite

Autolysis of purified Saccharomyces cerevisiae cell walls resulted in the release of several components thought to be mannoprotein in nature, since they were retained by Concanavalin ASepharose. The most abundant was a 29 kDal molecule which was also a major species among mannoproteins solubilized by the p-glucanase complex Zymolyase. There was a concomitant release of glucose and mannose oligosaccharides and of P-glucanase activity. Glucanase and protease inhibitory treatments considerably lowered the release of mannoproteins. The use of different protease inhibitors during autolytic incubations interfered with at least two proteases (one of them being a cysteine protease) apparently involved in the solubilization and partial degradation of mannoproteins from the walls.

show abstract

Section: Discussionmentioning

confidence: 99%

Autolytic Release of Mannoproteins from Cell Walls of Saccharomyces cerevisiae

1985

View full text Add to dashboard Cite

show abstract

“…The presence of mannoprotein complexes in the upper wall layer in yeast is well supported by the fact that synergistic action of proteases and ,B-glucanases is required to dissolve completely the walls of the intact cells (87,214,264,268,269). The effect of proteases can be replaced by thiol reagents but not by the action of purified bacterial a-mannanase; neither is the a-mannanase alone effective in substantial removal of the mannan located at the wall surface (268,269).…”

Section: Wall Architecturementioning

confidence: 99%

Biosynthesis of cell walls of fungi.

Farkas¹

1979

Microbiological Reviews

196

View full text Add to dashboard Cite

FORMATION OF CELL WALLS .120 General .120 Biosynthesis of Individual Wall Components .121 Chitin .121 Glucans .124 ten on this subject in the last years (e.g., 5, 9, 10, 15, 16, 205). However, some general explanations are necessary to serve as an introduction to the problem of fungal cell walls. Chemical Structure The remarkable properties of fungal walls, such as their mechanical strength, morphological features, and biological activity, are undoubtedly based on their particular chemical composition. Bartnicki-Garcia (15) pointed out that a close correlation exists between taxonomic classification and cell wall composition among fungi. Polysaccharides, which represent about 80 to 117

show abstract

“…Spheroplasts can be prepared from strains of Saccharomyces cereL'isiae by digesting cells with /8-glucanase-containing preparations, such as snail gut juice or various microbial enzymes (15,19). Strains of this yeast differ in their susceptibility to /8-glucanase digestion (9,17) and, with refractory strains, spheroplast formation can be accelerated by treating cells with sulfhydryl compounds (3,7) or proteolytic enzymes (20). Several transmission electron microscope studies of spheroplast formation from S. ceretuisiae have been published (e.g., ref.…”

mentioning

confidence: 99%

Scanning electron microscope study of Saccharomyces cerevisiae spheroplast formation

Pringle¹,

Forsdyke²,

Rose³

1979

J Bacteriol

View full text Add to dashboard Cite

A suspension of Saccharomyces ceretisiae NCYC366 in buffered 1.2 M sorbitol containing Zymolyase-5000 (a ,/-glucanase-containing preparation) showed maximum osmotic sensitivity after 30 min of incubation at 30°C. A scanning electron microscope study of spheroplast formation, using a very high resolution (4-nm) machine, revealed several new morphological features. The surface of the plug in bud scars on intact cells appeared warty. The wall, which assumed a beady appearance as digestion proceeded, ultimately sloughed off to reveal the furrowed surface of the plasma membrane. Bud scars were resistant to digestion and, as incubation proceeded, they became surrounded by an outer annulus, which may be the secondary septum. Wall material was completely removed from the majority of cells only after 60 min of digestion. The surface of spheroplasts was studded with particles, about 25 to 30 nm in diameter. Many spheroplasts had a single large indentation, which may be in that part of the plasma membrane originally underlying the birth scar.

show abstract

STUDIES ON THE FORMATION OF SPHEROPLASTS FROM STATIONARY PHASE CELLS OFSACCHAROMYCES CEREVISIAE

Cited by 18 publications

References 16 publications

Autolytic Release of Mannoproteins from Cell Walls of Saccharomyces cerevisiae

Autolytic Release of Mannoproteins from Cell Walls of Saccharomyces cerevisiae

Biosynthesis of cell walls of fungi.

Scanning electron microscope study of Saccharomyces cerevisiae spheroplast formation

Contact Info

Product

Resources

About