Studies on the flavones using liquid chromatography–electrospray ionization tandem mass spectrometry

Wu, Wen‐Hong; Yan, Chaochao; LI, L; LIU, Z; LIU, S

doi:10.1016/s0021-9673(04)01135-5

Cited by 104 publications

(54 citation statements)

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“…Results showed that terpene fraction possesses the highest activity at 40 µg ml at m/z 225 was most likely the loss of M-H-C 2 H 2 O-CO 2 , and further loss of C 2 H 2 O that corresponds to the ion m/z 183. This fragmentation pathway usually corresponds to compounds from the class flavonoids, probably for apigenin, as reported by Wu et al (2004) which showed such a fragmentation pathway. Peaks 9 and 10, which correspond to m/z 325 and 339 respectively, also shared similar fingerprint fragment ions (Table 1).…”

Section: Profiling Of Selected Potent Antioxidative Extract Constituesupporting

confidence: 53%

CUPRAC assay-guided profiling of antioxidant compounds in methanol extract of Lentinus squarrosulus Mont. mycelium

Abdullah

Sabaratnam

et al. 2018

AAS

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A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phasehigh performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A 450

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Section: Profiling Of Selected Potent Antioxidative Extract Constituesupporting

confidence: 53%

CUPRAC assay-guided profiling of antioxidant compounds in methanol extract of Lentinus squarrosulus Mont. mycelium

Abdullah

Sabaratnam

et al. 2018

AAS

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“…In particular, electrospray ionization multi‐stage tandem mass spectrometry (ESI‐MS n ) is a very efficient technique for identifying the structures of flavonoids . Relative to positive ion mode, negative‐ion mode have been proven to have a higher sensitivity and more readily provide flavone fragmentation information by CID, making it more effective for the structural characterization and isomeric differentiation of flavonoid glycosides . Therefore, based on the characteristic mass spectrometry fragmentation patterns of flavonoid glycosides, the retention times of references and their UV spectra, the structure of the unknown flavonoids in Dendrobium can be determined.…”

Section: Introductionmentioning

confidence: 99%

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

et al. 2019

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Dendrobium huoshanense, a unique species in the genus Orchidaceae, is only found in China and is known as “mihu”. Due to the lack of quality control, the use of D. huoshanense in the herbal market has been limited. In this study, methods based on thin‐layer chromatography, high‐performance liquid chromatography and high‐performance liquid chromatography coupled with electrospray ionization multi‐stage tandem mass spectrometry were used to identify the flavonoids in D. huoshanense and distinguish this species from other Dendrobium species. Using thin‐layer chromatography, a characteristic band was observed for D. huoshanense, and this band was absent from the thin‐layer chromatography plates of other Dendrobium species. Then, using high‐performance liquid chromatography, nine peaks of flavonoids were observed in the chromatograms of ten batches of D. huoshanense. Ultimately, 22 flavonoids in D. huoshanense were identified by multi‐stage tandem mass spectrometry, and 11 of these compounds are being reported from D. huoshanense for the first time. In addition, two compounds both with molecular weights of 710, were identified as being unique to D. huoshanense; one of these compounds, apigenin‐6‐C‐α‐L‐rhamnosyl‐(1→2)‐β‐D‐glucoside‐8‐C‐α‐L‐arabinoside, was proven to be responsible for the characteristic thin‐layer chromatography band of D. huoshanense. These analysis methods can be applied for the identification and quality control of D. Huoshanense.

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“…The utility of HPLC itself for separation of complex mixtures was greatly enhanced by mass spectrometric detection, which allowed the confident identification of, e.g., the flavonoids in plant materials. A number of papers dealing with LC/ESI‐MS analyses of flavonoids in plants have been published 8–29. Fabre et al 8.…”

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confidence: 99%

Liquid chromatography/electrospray ionization mass spectrometry for the characterization of twenty‐three flavonoids in the extract of Dalbergia odorifera

Liu

Guo

et al. 2005

Rapid Comm Mass Spectrometry

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A method incorporating high-performance liquid chromatography (HPLC) with electrospray ionization and tandem mass spectrometry, with parallel analysis by HPLC with UV detection using a diode-array detector, was developed for the qualitative characterization of flavonoids in D. odorifera. Twenty-three flavonoids, including six isoflavones, six neoflavones, four isoflavanones, three flavanones, two chalcones, one isoflavanonol and one pterocarpan, were unambiguously identified by comparing their retention times, UV and MS spectra with those of authentic compounds. Furthermore, the collision-induced dissociations of the [M-H]- ions were studied to clarify the MS behavior of the different types of flavonoids. In negative ion ESI-MS all the flavonoids yielded prominent [M-H]- ions in the first order mass spectra. Fragments involving losses of CH3*, H2O, CO, C2H2O, and CO2 were observed in the MS/MS spectra. Each of the seven types of flavonoid showed characteristic MS/MS fragmentation patterns. The isoflavanones, flavanones and chalcones were observed to undergo retro-Diels-Alder fragmentations. The spectra of almost all the neoflavonoids unexpectedly exhibited only [M-H-CH3]-* radical anions as base peaks without any further fragmentation. Substitution positions also remarkably influenced the fragmentation behavior, which could assist in distinction among the flavonoid isomers. The fragmentation rules deduced here could aid in the characterization of other flavonoids of these types.

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Studies on the flavones using liquid chromatography–electrospray ionization tandem mass spectrometry

Cited by 104 publications

References 0 publications

CUPRAC assay-guided profiling of antioxidant compounds in methanol extract of Lentinus squarrosulus Mont. mycelium

CUPRAC assay-guided profiling of antioxidant compounds in methanol extract of Lentinus squarrosulus Mont. mycelium

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

Liquid chromatography/electrospray ionization mass spectrometry for the characterization of twenty‐three flavonoids in the extract of Dalbergia odorifera

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