Summary.-Using competitive double -antibody radioimmunoassays we have shown that immunoglobulin (especially IgA) can be recovered in pH 3-5, 012M acid citrate eluates of freshly excised CCHl tumour-cell suspensions. Studies with 1251-labelled eluates indicate that such preparations exhibit a variable, but appreciable, degree of non-specific binding to unrelated syngeneic tumour and normal tissues. PAGE/SDS gel electrophoresis of the labelled eluates revealed the presence of a major nonimmunoglobulin component of 33-36K dalton which could account in part for the non-specific binding observed. This component was also detected in similar eluates from cultured CCH1 tumour and in all other tumour-cell eluates examined to date. In contrast, preliminary data suggest that it is less prevalent in acid citrate eluates from normal tissue, with the exception of peritoneal-exudate cells. The possible origins, nature and significance of this non -immunoglobulin component are discussed.