Studies on the Expression of Fibroblast Growth Factor-2 from Odontoblast-like Cells

Oliveira, Sandra Helena Penha de; Santos, Vanessa Aparecida Carvalho

doi:10.1016/j.joen.2011.08.004

Cited by 7 publications

(8 citation statements)

References 41 publications

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“…The assay (DuoSet ELISA development systems; R&D System) was performed in triplicate, according to the manufacturerl's recommendations. 45,[49][50][51] The quantitative analysis of gene expression for type IV collagen (COL IV; COL4A3BP; Hs00178621_m1; Thermo Fisher Scientific), matrix metalloproteinase 9 (MMP9; MMMP9: Hs00234579_m1; Thermo Fisher Scientific), and transforming growth factor b (TGFb; TGFB1:…”

Section: Discussionmentioning

confidence: 99%

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Effect of different methods of polymerizing ocular prosthesis acrylic resin on a human conjunctival cell line

Silva

Goiato

Bonatto

et al. 2016

The Journal of Prosthetic Dentistry

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Section: Discussionmentioning

confidence: 99%

“…Hs0099133_m1; Thermo Fisher Scientific) 49 was performed through real-time reverse-transcriptase (RT)-PCR. These targets are part of the tissue repair process.…”

Section: Discussionmentioning

confidence: 99%

Effect of different methods of polymerizing ocular prosthesis acrylic resin on a human conjunctival cell line

Silva

Goiato

Bonatto

et al. 2016

The Journal of Prosthetic Dentistry

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“…The collection aimed to dose the proinflammatory cytokines IL1b, IL6, and TNFa and the chemokine macrophage inflammatory protein 1a (CCL3/MIP1a), using enzymelinked immunosorbent assay (ELISA; DuoSet ELISA Development Systems; R&D System). 35,36 A total volume of 100 mL of cell-free supernatant was used for the quantitative analysis of the specimen in triplicate, performed according to the recommendations of the manufacturer. 35,36…”

Section: Obtaining Eluatesmentioning

confidence: 99%

“…35,36 A total volume of 100 mL of cell-free supernatant was used for the quantitative analysis of the specimen in triplicate, performed according to the recommendations of the manufacturer. 35,36…”

Section: Obtaining Eluatesmentioning

confidence: 99%

Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis

Bonatto

Goiato

Silva

et al. 2017

The Journal of Prosthetic Dentistry

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“…Intriguingly, some pharmacological activities of AS‐IV result from AS‐IV‐mediated inhibition of the ERK and NF‐κB signaling pathways . Basic fibroblast growth factor, also known as bFGF, FGF2, or FGF‐β, one of the pro‐inflammatory factors which can be induced by LPS in odontoblast‐like cells, has been reportedly involve in the regeneration and differentiation of dental pulp cells . Moreover, the expression of FGF2 can be reduced by AS‐IV treatment .…”

Section: Introductionmentioning

confidence: 99%

Astragaloside IV attenuates inflammatory injury and promotes odontoblastic differentiation in lipopolysaccharide‐stimulated MDPC‐23 cells and rat pulpitis

Ding¹,

Gao

Zheng³

et al. 2019

J Oral Pathology Medicine

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Background Astragaloside IV (AS‐IV), a natural herbal compound from Astragalus membranaceus, has inhibitory effects on receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclastogenesis, and RANKL signal helps to regulate odontoblast differentiation. However, whether and how AS‐IV affects odontoblastic differentiation remains unclear. Methods Lipopolysaccharide (LPS)‐stimulated MDPC‐23 cells and rat pulpitis were treated with AS‐IV, cell viability, and LDH leakage was analyzed by CCK‐8 assay and LDH Leakage assay. The production of TNF‐α and IL‐6 was determined by ELISA and qRT‐PCR assay. The expression of alkaline phosphatase (ALP) was detected using an ALP assay kit, and the expression of dentin sialophos‐phoprotein (DSPP), dentin matrix protein‐1 (DMP1), basic fibroblast growth factor (FGF2), and phosphorylated extracellular signal‐regulated kinase (p‐ERK) was determined by western blot. Results AS‐IV dose dependently increased in cell viability and decreased the overproduction of TNF‐α and IL‐6 in LPS‐stimulated MDPC‐23 cells. AS‐IV also counteracted LPS‐induced downregulation of ALP, DSPP, and DMP1 in MDPC‐23 cells. Furthermore, AS‐IV significantly decreased the expression of FGF2 and p‐ERK in LPS‐stimulated MDPC‐23 cells. More important, the addition of FGF2 partly neutralized AS‐IV‐mediated inhibition of FGF2/ERK signaling, abolished AS‐IV‐induced reduction of TNF‐α and IL‐6, and counteracted AS‐IV‐induced upregulation of DSPP and DMP‐1 in these cells. Meanwhile, AS‐IV inhibited the excessive production of TNF‐α and IL‐6, suppressed the downregulation of DSPP and DMP1, and disturbed the up‐regulation of FGF2 and p‐ERK in the pulp tissues of rat pulpitis model. Conclusions AS‐IV exerted anti‐inflammatory and pro‐differentiation effects in LPS‐stimulated MDPC‐23 cells and rat pulpitis via inhibiting the FGF2/ERK signaling pathway.

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Studies on the Expression of Fibroblast Growth Factor-2 from Odontoblast-like Cells

Cited by 7 publications

References 41 publications

Effect of different methods of polymerizing ocular prosthesis acrylic resin on a human conjunctival cell line

Effect of different methods of polymerizing ocular prosthesis acrylic resin on a human conjunctival cell line

Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis

Astragaloside IV attenuates inflammatory injury and promotes odontoblastic differentiation in lipopolysaccharide‐stimulated MDPC‐23 cells and rat pulpitis

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