Studies on the Effects of Adult Animal Tissue Extracts on Wound Healing

Hoffman, R. S.; Dingwall, James A.; Andrus, William DeW.

doi:10.1097/00000658-194612000-00014

Cited by 7 publications

(5 citation statements)

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“…So far, it is evident that there is at least one growth promoting factor in the euglobulin fraction of cord serum. This factor would seem to correspond to the growth-promoting factor described by Baker and Carrel (6) and Carrel and Ebeling (7) in the euglobulin fraction of chicken serum and perhaps to the protein fraction isolated from sheep heart by Hoff man, Dingwall, and Andrus (8).…”

Section: Discussionmentioning

confidence: 55%

“…Baker and Carrel (6) and Carrel and Ebeling (7) isolated a euglobulin fraction from chicken serum which had growth-promoting activity. Hoffman, Dingwall, and Andrus (8) obtained an active protein fraction from an extract of sheep heart and Davidson and Waymouth (9) obtained active protein fractions from brain, heart, and skeletal muscle of sheep embryos. Laser (10) found that tissue cultures fed only * This work has been aided by a grant from the American Cancer Society and by a grant and fellowship from the Division of Research Grants and Fellowships of the Public Health Service 765 embryo extract showed a greater increase in area than cultures fed only serum; however, the increase in weight was greater in the cultures that were fed only serum.…”

Section: Introductionmentioning

confidence: 99%

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Tissue Culture Media

Jacquez

Barry

1951

Journal of General Physiology

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1. Human placental cord serum contains a factor or factors necessary for active growth of fibroblasts in tissue culture and a factor which prevents the appearance of a characteristic toxicity. 2. The growth-promoting factor(s) is stable to heating to 60°C. for 30 minutes and is associated with the euglobulin fraction of the serum. 3. Extraction of dried cord serum with benzene, chloroform, and ether does not cause any decrease in growth-promoting activity. 4. Sera from hypophysectomized, adrenalectomized, and thyroid-parathyroidectomized rats show as much growth-promoting activity as normal rat sera. 5. Bovine gamma globulins, anterior pituitary growth hormone, and insulin do not replace the serum growth factor. 6. The toxicity-preventing factor is serum albumin. 7. Evidence is presented that the toxicity is due to free fatty acids in the medium and that serum albumin acts by binding the fatty acids.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Tissue Culture Media

Jacquez

Barry

1951

Journal of General Physiology

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“…Further testing also revealed that adult tissue extracts affect both connective and epithelial tissue . Finally, Werner and Doljanski produced a powder preparation of sheep's heart, and this set the stage for the test of Hoffman et al . as noted previously.…”

Section: Tissue Extractsmentioning

confidence: 82%

“…The first study assessing the effects of heart extract preparation (HEP) on wound healing in an animal model was by Hoffman et al., wherein they reported a 40% acceleration of skin wound healing in dogs serving as their own control. This study resulted from the efforts of Doljanski and Hoffman starting in 1939, who wanted to apply embryonic tissue extracts to enhance wound healing following the suggestions of Carrel and Burrows and Carrel .…”

Section: Tissue Extractsmentioning

confidence: 99%

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Historical foundations of wound healing and its potential for acceleration: dose‐response considerations

Calabrese

2013

Wound Repair Regeneration

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This paper provides a detailed historical assessment of the origin and developmental progress of the concept of wound healing and its attempted acceleration from its start in the beginning of the 20th century to approximately 1960. Emphasis is placed on the development of cell culture in the assessment of wound healing and in attempts to validate experimental findings via clinical research. Of particular interest were the observations that wound healing could be accelerated in the 30-50% range with the dose response displaying biphasic characteristics consistent with the hormesis dose-response model. Such findings set the stage for the hormetic dose-response revolution that is occurring within the biological and biomedical sciences, including wound healing, whereby considerable research now supports the capacity for endogenous and exogenous agents to accelerate the process of wound healing and its functional performance.

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Untersuchungen �ber tierische Wundhormone

Hegemann¹,

Traut²,

Wallenstern³

1950

Arch. f. klin. Chir

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Bei den Betrachtungen fiber die Ursache der ~eparation nach einer Defektsetzung im lebenden Gewebe ist yon vielen Biologen die Hypothese gefordert worden, dab der wichtigste, hierbei wirksame, formative Stimulus chemischer Natur sei. Einen wesentliehen Fortschritt in dieser Diskussion brachten die Beflmde I-~ABERLANDTS (1921) fiber p/lanzliche ,, W undhormone" .Er kolmte zeigen, d~g an frischen Schnittfl~chen yon Kohlrabi und Kartoffelh knollen sehr reichlich mitotisehe Zellteilungen erfolgen, dal~ diese Vorg/~nge aucr dutch Gewebsbrei ausgelSst werden kfnnen, abet naeh Abspttlen wegbleiben. Eund andere Pttanzenphysiologen bewiesen in vielen Versuehsanordnungen, dag be-sehSAigte oder abgestorbene Zellen Substanzen abgeben, die bei Einwirkung auf nichtverwundete Gewebsbezirke Zellteilungen und Zellstrec~ungen auslSsen. Derartige Stoffe werden Wund-, Teilungs-oder Nekrohormone genannt. Neuerdings konnte ein solches pfl~nzliehes Wundhormon, z.B. aus Bohnen gewonnen, seine Konstitution aufgekl~rt, es sogar synthetisiert werden (E~GI~ISH 1941). Es h~ndel~ sieh um die zweibasige unges~ttig~e Fettsi~ure; das Traum~tin. Wahrscheinlich sind die pflanzlichen Wundhormone chemisch nicht einheitlieh. Eiweil]abbauprodukte, z. B. das Tyrosin, sollen iihnlich wirken kfnnen wie das Traumatin. Die Ermittlung der chemisehen N~tur wird erschwert dureh die Tatsache, dab sehr viele Substanzen, die keine Wundhormone sind, in hSheren Konzentrationen zellteilungs-auslSsend auf pflanzliches Gewebe wirken. Sic tun das, well sie d~s physiologisehe GMchgewicht irgendwie stfren und durch einen geringen ,,Verwundungsreiz" die kSrpereigenen, eigentliehen Wundhormone freisetzen (i)bersicht bei HA~TMA~, B/~ZUIJSG). Auf die in den letzten Jahren besonders erfolgreich studierten Wnehsstoffe bestimmter Vitamine (Biosgruppe) und auf die vielen neuerdings als Naturstoffe isolierten und synthetisch gewonnenen, bei niedrigen Organismen wirkenden Wuehssto//e (Ubersicht bei RUDOLPH), soll hier far nicht eingegangen werden, da wesentliche Beziehungen zu tierischen Wundheilungsvorg~ngen bisher zu fehlen seheinen.Es sei sehon zu Beginn unserer Ausftihrungen betont, dal3 gegenfiber den Befunden der Pflanzenphysiologen, die den grundsatzlieh wichtigen Beweis eines zellteilungswirksamen Wundhormons liefern kSnnen, atle Feststellungen fiber tierische Wundhormone weniger exakt definiert sind. Dies liegt damn, dab es viel sehwieriger ist, standardisierte tierisehe tndividuen als Test in geni~gender Zahl zu erhalten als ein leieht * IIerrn Prof. A. LAEW~S zum 75. Geburtstag.

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Studies on the Effects of Adult Animal Tissue Extracts on Wound Healing

Cited by 7 publications

References 1 publication

Tissue Culture Media

Tissue Culture Media

Historical foundations of wound healing and its potential for acceleration: dose‐response considerations

Untersuchungen �ber tierische Wundhormone

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