Studies on the effect of ethanol on eukaryotic protein synthesis in vitro.

David, E T; Fischer, Itzhak; Moldave, Kivie

doi:10.1016/s0021-9258(18)32236-1

Cited by 20 publications

(2 citation statements)

References 11 publications

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“…The inhibition of translation and the downregulation of genes encoding ribosomal subunits are well-described and conserved impacts of alcohol exposure in vitro and in a variety of species from bacteria to humans. 47 – 52 In addition, reproductive pathways were among the most affected across exposure conditions at the F1, such as “gamete generation,” “germ cell development,” and “embryo development ending in birth or egg hatching” pathways ( Table S4 ).…”

Section: Resultsmentioning

confidence: 99%

Single-nucleus resolution mapping of the adult C. elegans and its application to elucidate inter- and trans-generational response to alcohol

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Single-nucleus resolution mapping of the adult C. elegans and its application to elucidate inter- and trans-generational response to alcohol

et al. 2023

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“…However, a major target of ethanol across exposure conditions and clusters is the translation machinery as exemplified by the deregulation of many ribosomal components and representing 5 of the top 10 GO categories, including the top 3 (Table S4). The inhibition of translation and the downregulation of genes encoding ribosomal subunits are a well described and conserved impact of alcohol exposure in vitro and in a variety of species from bacteria to humans [50][51][52][53][54][55]. In addition, reproductive pathways were amongst the top shared across exposure conditions at the F1, such as "gamete generation", "germ cell development", and "embryo development ending in birth or egg hatching" pathways (Table S4).…”

Section: Snrna-seq Reveals Broad Impacts Of Inter-generational Exposu...mentioning

confidence: 99%

Single-nucleus resolution mapping of the adult C. elegans and its application to elucidate inter- and trans-generational response to alcohol

Truong¹,

CHEN

Barrere‐Cain³

et al. 2022

Preprint

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Single-cell RNA transcriptomic platforms have significantly contributed to our understanding of tissue heterogeneity as well as of developmental and cellular differentiation trajectories. They also provide an opportunity to map an organism’s response to environmental cues with high resolution and unbiasedly identify the cell types that are the most transcriptionally sensitive to exposures. Here, we applied single nucleus RNA-seq experimental and computational approaches to C. elegans to establish the transcriptome of the adult nematode and comprehensively characterize the transcriptional impact of ethanol as a model environmental exposure on the entire organism at cell type-resolution over several generations. Clustering, tissue and phenotype enrichment, and gene ontology analyses identified 31 clusters representing a diverse number of adult cell types, including those from syncytial and multi-nucleated tissues which are difficult to assess by single cell RNA-seq, such as the mitotic and meiotic germline, hypodermal cells, and the intestine. We applied this method to identify the impact of inter- and trans-generational exposure to two human-relevant doses of alcohol. Cell type proportions were not significantly altered by ethanol. However, Euclidean distance analysis identified several germline, striated muscle, and neuronal clusters as being major transcriptional targets of ethanol at both the F1 and F3 generations although the relative order of clusters changed between generations. The impact on germline clusters was further confirmed by phenotypic enrichment analysis as well as functional validation, namely a remarkable inter- and trans-generational increase in germline apoptosis, aneuploidy, and embryonic lethality. Together, snRNA-seq of the adult C. elegans represents a powerful approach for the detailed examination of an adult organism’s response to environmental cues.

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Serum albumin

Rothschild¹,

Oratz²,

Schreiber³

1988

Hepatology

405

278

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The liver manufactures albumin at a massive rate and decreases production in times of environmental, nutritional, toxic and trauma stress. Osmotic pressure is a basic evolutionary regulatory factor, and hormonal control over albumin production has been demonstrated. Where and why new or old albumin is degraded are questions which have not been clarified, although the vascular endothelium may well be the degradative site. Albumin is important as a transport protein, as a measure of evolution and as a model to study secretion following synthesis without the intervening steps of glycosylation. Investigations as to how this protein enters the endoplasmic membrane may well answer some of the questions concerning signal peptide insertion (288). The role of the urea cycle intermediate ornithine and its participation in polyamine synthesis, which has a positive effect on albumin synthesis, is under study. Likewise, the inverse relation between acute-phase protein synthesis and albumin synthesis regulated by interleukin 1 and other cytokines will merit further study. These are a few of the concepts which will be tested in the future.

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Studies on the effect of ethanol on eukaryotic protein synthesis in vitro.

Cited by 20 publications

References 11 publications

Single-nucleus resolution mapping of the adult C. elegans and its application to elucidate inter- and trans-generational response to alcohol

Single-nucleus resolution mapping of the adult C. elegans and its application to elucidate inter- and trans-generational response to alcohol

Single-nucleus resolution mapping of the adult C. elegans and its application to elucidate inter- and trans-generational response to alcohol

Serum albumin

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