Studies on the effect of peroxisomicine on catalase activity in albino mice

Effect of peroxisomicine A2 and T 544 of the genus Karwinskia on peroxisomes of Candida boidinii

“…We have previously reported the inhibitory e¡ect in vitro of structurally related anthracenones, among them peroxisomicine A I , peroxisomicine A P and T 544, on liver catalase activity [6]. Recently, we also demonstrated that peroxisomicine A I was not able to inhibit the catalase activity in intoxicated mice, in vivo [7]. This is in agreement with the ¢ndings described by Sepu è lveda et al [5], which indicate that catalase activity is not modi¢ed in methylotrophic yeasts with peroxisomal damage induced by peroxisomicine A I in vivo.…”

“…Recently, we reported the inhibitory e¡ect in vitro of peroxisomicine A I and structurally related anthracenones on liver catalase activity, using the puri¢ed enzyme [6]. In contrast to the described ¢ndings in vitro, peroxisomicine A I is not able to inhibit in vivo the activity of catalase in methylotrophic yeasts with peroxisomal damage induced by the toxin [5], either in hepatic fragments incubated with this toxin (in situ) or in intoxicated mice (in vivo) [7]. Considering the reported e¡ect of peroxisomicine A I on peroxisomes of methylotrophic yeast cells, and the structural similarity of other dimeric anthracenones, we were interested in investigating whether this e¡ect is exclusive to peroxisomicine A I .…”

Effect of peroxisomicine A2and T 544 of the genusKarwinskiaon peroxisomes ofCandida boidinii

“…We have previously reported the inhibitory effect in vitro of structurally related anthracenones, among them peroxisomicine A 1 , peroxisomicine A 2 and T 544, on liver catalase activity [6]. Recently, we also demonstrated that peroxisomicine A 1 was not able to inhibit the catalase activity in intoxicated mice, in vivo [7]. This is in agreement with the findings described by Sepúlveda et al [5], which indicate that catalase activity is not modified in methylotrophic yeasts with peroxisomal damage induced by peroxisomicine A 1 in vivo.…”

“…Recently, we reported the inhibitory effect in vitro of peroxisomicine A 1 and structurally related anthracenones on liver catalase activity, using the purified enzyme [6]. In contrast to the described findings in vitro, peroxisomicine A 1 is not able to inhibit in vivo the activity of catalase in methylotrophic yeasts with peroxisomal damage induced by the toxin [5], either in hepatic fragments incubated with this toxin (in situ) or in intoxicated mice (in vivo) [7]. Considering the reported effect of peroxisomicine A 1 on peroxisomes of methylotrophic yeast cells, and the structural similarity of other dimeric anthracenones, we were interested in investigating whether this effect is exclusive to peroxisomicine A 1 .…”

Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy

“…For this reason, T‐514 was renamed as peroxisomicine A1 (PA1). Peroxisomes play an active role in cancer development (for a review, see Dahabieh et al, 2018), and a hypothesis exists (Moreno‐Sepúlveda et al, 1997) that, as PA1 attacks peroxisomes, cells with fewer peroxisomes such as tumor cells from a significant number of neoplastic tissues (reviewed by Islinger et al, 2018), would be destroyed more easily than normal cells.…”