Studies on the digestibility and utilization of the nitrogen of irradiated rumen bacteria by rats

Mason, V. C.; Palmer, Robert M.

doi:10.1017/s0021859600069574

Cited by 28 publications

(7 citation statements)

References 14 publications

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“…Only diaminopimelic acid (DAPA) was very low, as expected (Mason & Palmer, 1971;Mason & White, 1971). Of the other AA, only histidine (0.68), cystine (0.73) and proline (0.76) had values substantially less than the average.…”

Section: Resultssupporting

confidence: 72%

The nutritive value of rumen micro-organisms in ruminants

Storm¹,

Brown²,

Ørskov³

1983

Br J Nutr

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1. An experiment was conducted with three sheep maintained entirely by intragastric nutrition to estimate the digestibility of isolated individual constituents and amino acids (AA) of rumen micro-organisms (RMO) in the small intestine.2. Five levels of RMO were infused into the abomasum. The apparent and true disappearance of the individual components were measured by regression of abomasal input on the passage at the ileum. In order to proceed further to state the AA requirement of ruminants precisely it is also necessary to estimate the digestibility of each AA from RMO since this is the main protein source for ruminants. The present paper describes the estimation by regression analysis of the true digestibilities of total N, total AA-N, RNA, DNA and nineteen individual AA of RMO and the endogenous losses of these substances in the small intestine of three sheep sustained entirely by a constant intraruminal infusion of volatile fatty acids (VFA) and minerals and abomasal infusion of five different amounts of RMO. A preliminary account of some of this work has been given previously (Storm & Orskov, 1982). MATERIALS A N D METHODS Animals.Three Suffolk x (Finnish Landrace x Dorset Horn) wether lambs, weighing between 27 and 36 kg, were used.Each animal was surgically prepared with a permanent rumen cannula and an abomasal catheter, as previously described by Orskov et al. (1979). Furthermore, the animals were fitted with a simple T-piece cannula in the distal part of the ileum, 15-20 mm anterior to the ileo-caecal junction. The open end of the ileal cannula was exteriorized midway up on the right flank of the animal and a plastic loop arranged on each side of the cannulas according to Hecker (1974) so that total collection of ileal fluid was possible.Infusion treatment. After the lambs had been fitted with the cannulas they were kept in individual metabolism cages for 2 weeks before intragastric infusion was commenced. The infusion regimen was then gradually introduced over 10-12 d. Clean drinking water was freely available at all times. 16-2

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Section: Resultssupporting

confidence: 72%

The nutritive value of rumen micro-organisms in ruminants

Storm¹,

Brown²,

Ørskov³

1983

Br J Nutr

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“…This value is at the lower end of the range of reported nitrogen values for mixed rumen populations (Milwid et al, 1968;Mason and Palmer, 1971). About 40% of the faecal nitrogen in this study appears to be in the soluble fraction of the solids.…”

Section: Discussionsupporting

confidence: 43%

The Microbial Contribution to Human Faecal Mass

Stephen

Cummings

1980

Journal of Medical Microbiology

480

231

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“…Nevertheless, estimates for the BV, of RMO obtained with rats are generally higher than our estimates. A possible reason for the discrepancy may be an active hind-gut fermentation (Mason & Palmer, 1971) as outlined previously. It is interesting to note that the estimates of N digestibility and BV, obtained by Mason & Palmer (1971), who conducted the largest and most comprehensive of the rat experiments published, are in best agreement with our values obtained with ruminants.…”

Section: Non-ruminant In Vivo Methodsmentioning

confidence: 97%

“…However, the results with rats are variable and this may reflect differences in experimental methods used, such as the method of drying, e.g. freeze-drying (Mason & Palmer, 1971), extraction with alcohol and drying at 38' (McNaught et af. 1954).…”

Section: Non-ruminant In Vivo Methodsmentioning

confidence: 99%

“…Furthermore, the AA composition of this material appears to be constant irrespective of dietary and animal conditions (Storm et al 1983). It has been difficult to obtain precise estimates of the nutritive value of microbial N for ruminants due to difficulties in isolating sufficient quantities of rumen micro-organisms (RMO) for experiments with large animals, though some evaluations of nutritive value have been undertaken using rats (Johnson et al 1944;McNaught et al 1954; Mason & Palmer, 1971).…”

mentioning

confidence: 99%

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