Studies on the Determination of Biogenic Amines in Foods. 1. Development of a TLC Method for the Determination of Eight Biogenic Amines in Fish

Changes in pork and shark (Rhizopriondon terraenovae) protein emulsions due to exogenous and endogenous proteolytic activity

“…2), where pH remained within a closer range (5.95 ± 0.05 to 6.63 ± 0.06) to pH for maximum activity of one protease (pH 6.0). Removing shark meat from its natural environment promotes rapid deterioration producing amines which, in turn, increase pH (Naguib et al, 1995).…”

“…Naguib, Ayesh, and Shalaby (1995) found unspecific protein breakdown; whereas Alanís et al (1999) reported that protease extracts obtained from Pseudomonas fluorescens and Pseudomonas fragi mainly act on actin and high molecular weight intermediate proteins. In addition of Pseudomonas spp.…”

Determination of biogenic amines in oysters by capillary electrophoresis coupled with electrochemiluminescence

“…Among those were high performance liquid chromatography (HPLC) (Yen, & Hsieh, 1991;Li et al, 2014), capillary electrophoresis (CE) (Park et al, 2010), ion chromatography (IC) (Cinquina et al, 2004;Palermo, Muscarella, Nardiello, Iammarino, & Centonze, 2013), thin-layer chromatography (TLC) (Naguib, Ayesh, & Shalaby, 1995;Romano et al, 2012) and gas chromatography (GC) (Naila, Flint, Fletcher, Bremer, & Meerdink, 2011). HPLC requires extensive sample preparation, including derivatization of the amines to form non-polar volatile compounds (the highly polaramines tend to ''stick'' to the column and cause ''memory effects'') (Callejón, Sendra, Ferrer, & Pardo, 2013).…”

Phenols, Aromatic Carboxylic Acids, and Indoles

“…Adsorption and partition chromatographic TLC methods have been comprehensively studied for the analysis of biogenic amines and alkaloids and their derivatives (225b). A procedure has been developed for the determination of eight biogenic amines in fish (226). The methodology for the separation, detection, and quantitative determination of catecholamines, 5-hydroxytryptamine, and their metabolites in biological samples has been reviewed (227).…”