Studies on the Determination of Species and Cultivars on the Basis of Electrophoretic Patterns of Seed Protein and Seed Enzymes

Nakamura, Suguru; Tahara, Mochimu

doi:10.2503/jjshs.46.233

Cited by 13 publications

(2 citation statements)

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“…The work of Larsen (1966) and Nakamura (1979) indicated that banding patterns obtained by the electrophoretic separation of seed proteins under non-denaturing conditions might be used to identify some grass cultivars, because they were able to distinguish seed of perennial and Italian ryegrasses. More recently, De Prins & Van de Weghe (1983), by electrofocussing the urea-soluble ryegrass seed proteins were able to readily differentiate between perennial and Italian ryegrasses and de~on~trated distinctive banding patterns of these protems m seed extracts from 8 different cultivars.…”

Section: Introductionmentioning

confidence: 98%

Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis

Gardiner¹,

Forde²,

Slack³

1986

New Zealand Journal of Agricultural Research

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The banding pattern of seed storage proteins resulting from sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) of extracts from bulked seed samples has been used to distinguish species and cultivars within the genera Latium and Festuca. Perennial (L. perenne L.) and Italian (L. multiflorum Lam.) ryegrasses were readily differentiated, and distinctive banding patterns were obtained for seed proteins from a range of cultivars and varieties of each, some very closely related. These patterns were independent of site, year, or generation of seed production. Fescue species differentiated were F. pratensis Huds. (meadow fescue), F. arundinacea Schreb. (tall fescue), and F. rubra L. (creeping red and Chewings fescues). The 2 types within F. rubra could be distinguished. SDS-PAGE of bulked seed samples offers a rapid method for distinguishing cultivars of Latium and Festuca during seed certification, for checking for genetic shift or contamination in cultiv.ars ~u~ng seed multiplication, as well as for Identlfymg unknown or wrongly named lines. Seed protein banding pattern has potential as a tool for taxonomic classification and could also be used as an adjunct to the more traditional chara~ters ~sed in distinguishing cui ti vars for Plant Vanety Rights.

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Section: Introductionmentioning

confidence: 98%

Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis

Gardiner¹,

Forde²,

Slack³

1986

New Zealand Journal of Agricultural Research

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“…A seed esterase isoenzyme (Nakamura 1979;Payne et al 1980;Griffith and Banowetz 1992), like the electrophoresis of other general seed proteins and isozymes, was able to detect species differences but these associations were based on bulked samples and lacked the necessary sensitivity to be an adequate replacement test (Larsen 1966;Ferguson and Grabe 1984). Morphologically, leaf vernation differences between perennial and Italian ryegrass have been used (Grabe 1998;Okora et al 1999), but this test is time consuming and can be inconclusive.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of superoxide dismutase (Sod-1) in a perennial × annual ryegrass cross and its allelic distribution among cultivars

Warnke

Barker

Brilman³

et al. 2002

Theor Appl Genet

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Identifying annual ryegrass contamination in perennial ryegrass seed lots has been of major interest in seed-testing laboratories and for seed regulatory agencies in the USA for many years. This study was conducted to characterize a superoxide dismutase locus ( Sod-1) and determine its potential to distinguish cultivated ryegrass species. The inheritance of Sod-1 was evaluated in a three-generation annual x perennial ryegrass mapping population and segregation fitted an expected 1:2:1 ratio for a single locus with two alleles. The molecular form of the Sod-1 locus was determined by H(2)O(2) and KCN inhibitor assays which indicated that the Sod-1, and a second independently segregating Sod-2, locus were both Cu/ZnSod enzymes. The common alleles at the Sod-1 locus were scored in 13 annual and 24 perennial ryegrass cultivars to determine the potential of using this locus for species separation. The Sod-1b allele was homozygous in 98% of perennial ryegrass individuals from 24 cultivars, but those not 100% homozygous for Sod-1b were seed lots with unknown contamination from annual ryegrass. These results indicate that the Sod-1b allele in the homozygous condition is a good indicator of perenniality. All eight annual ryegrass cultivars originating in Europe or Asia had a low frequency of Sod-1b homozygous individuals or none at all. The five cultivars originating in the Western Hemisphere, however, had genotype frequencies for homozygous Sod-1b of up to 56%. The potential of the Sod-1 locus to serve as a test to separate the two growth forms depends on the source of the annual-type contamination.

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The characterisation and identification of crop cultivars by electrophoresis

Cooke

1984

Electrophoresis

114

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Studies on the Determination of Species and Cultivars on the Basis of Electrophoretic Patterns of Seed Protein and Seed Enzymes

Cited by 13 publications

References 1 publication

Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis

Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis

Inheritance of superoxide dismutase (Sod-1) in a perennial × annual ryegrass cross and its allelic distribution among cultivars

The characterisation and identification of crop cultivars by electrophoresis

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