Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis

Abstract

The banding pattern of seed storage proteins resulting from sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) of extracts from bulked seed samples has been used to distinguish species and cultivars within the genera Latium and Festuca. Perennial (L. perenne L.) and Italian (L. multiflorum Lam.) ryegrasses were readily differentiated, and distinctive banding patterns were obtained for seed proteins from a range of cultivars and varieties of each, some very closely related. These patterns were independent of site, year, or generation of seed production. Fescue species differentiated were F. pratensis Huds. (meadow fescue), F. arundinacea Schreb. (tall fescue), and F. rubra L. (creeping red and Chewings fescues). The 2 types within F. rubra could be distinguished. SDS-PAGE of bulked seed samples offers a rapid method for distinguishing cultivars of Latium and Festuca during seed certification, for checking for genetic shift or contamination in cultiv.ars ~u~ng seed multiplication, as well as for Identlfymg unknown or wrongly named lines. Seed protein banding pattern has potential as a tool for taxonomic classification and could also be used as an adjunct to the more traditional chara~ters ~sed in distinguishing cui ti vars for Plant Vanety Rights.

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“…Values of p˂ 0.05 were considered to be statistically significant. A great deal of attention and efforts have been paid to the development of biochemical methods for cultivar characterization, variety discrimination and identification (Gardiner et al, 1986;Cai and Bulletin 1992;El-Kariem 2002, 2003;Oladipo et al, 2008). The most widely applied and versatile technique used for laboratory cultivar characterization has been the analysis of seed and leaf proteins and enzymes by various forms of electrophoresis.…”

Comparison of Agronomic Characters, Total Seed Storage Proteins and their use for Genotypes Discrimination in the Kentucky Bluegrass (Poa PratensisL.)

“…Doyle et al (11) has successfully used DNA data in grass taxonomy and phylogeny. Composition of seed protein is generally not influenced by environmental factors; therefore, they undergo a slower process of evolution due to their non-essential nature in contrast to leaf proteins, and may give better results in the genetic diversity studies (9,15). Currently, biochemical methods especially SDS-PAGE (including electrophoretic analysis of endosperm proteins) is widely used in studies on phenotypically close taxa because SDS-PAGE is a simple and effective method for describing the genetic structure of crop germplasm (4,17,20).…”

“…Grass seed storage proteins have already been investigated systematically by several authors (16,26). Seed protein electrophoresis was found to be a useful technique for cultivar discrimination in several economically important grass species including Kentucky bluegrass (Poa pratensis l.) and ryegrass (L. perenne l. and L. multiflorum l.) (8,12,13,14,15,20,21,28). it is possible to identify and certify individual genotypes to analyze the progenies and populations (2,18,24,27).…”

Identification of timothy cultivars by SDS-PAGE analysis of seed storage proteins

“…) and pasrure grasses (Gardiner et al 1986;Gardiner and Forde 1987), as well as some other crops (Barratt 1980;Kapse and Nerkar 1985;and Onokpise et al 1988). …”