Comparison of Agronomic Characters, Total Seed Storage Proteins and their use for Genotypes Discrimination in the Kentucky Bluegrass (<i>Poa Pratensis</i>L.)

Tamkoç, Ahmet; Arslan, Emine

doi:10.2478/v10133-010-0007-5

Cited by 6 publications

(5 citation statements)

References 9 publications

(15 reference statements)

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“…Molecular analysis, in conjunction with the characteristics of morphological traits, may be used to classify plants and select the initial forms for creating new cultivars or breeding lines. In the case of P. pratensis , this was confirmed by Shortell, Meyer & Bonos (2009) , Tamkoc & Arslan (2010) , Raggi et al (2015) and Yuan et al (2015) . In this study, two markers (RAPD and ISSR) based on DNA amplification reactions were used to assess the genetic diversity of ecotypes and cultivars of P. pratensis .…”

Section: Discussionmentioning

confidence: 76%

“…It should be stressed that in previous studies aimed at evaluating RAPD polymorphism in different populations of P. pratensis , such a high genetic diversity among ecotypes had never been found, while the average Dice’s coefficient was about 0.7 ( Szenejko & Rogalski, 2015 ). Other authors also reported significant genetic relatedness among the different genotypes and cultivars of P. pratensis ( Johnson et al, 2002 ; Ning et al, 2005 ; Tamkoc & Arslan, 2010 ; Fard et al, 2012 ). UPGMA dendrograms based on genetic similarity matrices revealed discrepancies in the mode of clustering of the studied forms of P. pratensis .…”

Section: Discussionmentioning

confidence: 89%

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Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Szenejko

Śmietana

Stępień

2016

PeerJ

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BackgroundPoa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation.MethodsThe molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA).ResultsThe low value of Dice’s coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin.DiscussionThe populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 89%

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Szenejko

Śmietana

Stępień

2016

PeerJ

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“…RAPD analyses of Poa pratensis, primarily, focused on: identification of sexual and apomictic genotypes (Huff & Bara 1993;Mazzucato et al 1995;Barcaccia et al 1997Barcaccia et al , 1998Stephens et al 2006), assessment of genetic diversity, identification of different genotypes and cultivars (Lickfeldt et al 2002;Al-Humaid & Motawei 2004;Ning et al 2005;Liang et al 2009;Fard et al 2012;Wang et al 2012), attempts to determine the genetic relationships between different genotypes and species belonging to the genus Poa (Johnson et al 2002;Curley & Jung 2004;Patterson et al 2005), and identification of pathogens responsible for fungal diseases in grasses (Hsiang et al 2000). As evidenced by the results of research presented in the literature (Johnson et al 2002;Ning et al 2005;Rajasekar et al 2005;Szenejko et al 2009;Szenejko 2013) various forms of Poa pratensis are characterized by a high level of band polymorphism, estimated to be over 80%. This was confirmed by the results of RAPD analyses presented in this study.…”

Section: Discussionmentioning

confidence: 99%

Characterization of morphological traits and RAPD polymorphism in selected forms of Kentucky bluegrass (Poa pratensis L.)

Szenejko

Rogalski

2015

Biodiversity Research and Conservation

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Measurements were taken of seven morphological characteristics of various forms of Poa pratensis, including ecotypes (12), cultivars (4), and breeding lines (2). Statistically significant differences relating to the average number and length of vegetative shoots and the leaf width of the forms studied were determined using Tukey’s HSD test. Evaluation of genetic polymorphism was carried out using the RAPD-PCR method. 18 primers were used for molecular analysis and 473 amplification products were obtained, in which high levels of polymorphism (84.2%) and specificity (63) were discovered. The mean value of Dice’s coefficient, the genetic similarity indicator, was about 0.7. These results indicate a significant genetic similarity between most of the forms of Poa pratensis studied. A correlation was also observed between genetic variation and phenotypic diversity of the studied forms of Poa pratensis. Convergence was found to occur between phenotype and genetic similarity among the forms. Only the dos01 468 ecotype showed discrepancies in the assessment of phenotypic and genomic variability.

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“…Molecular analysis, in conjunction with the characteristics of morphological traits, may be used to classify plants and select the initial forms for creating new cultivars or breeding lines. In the case of P. pratensis, this was confirmed by Shortell et al (2009), Tamkoc & Arslan (2010), Raggi et al (2015) and Yuan et al (2015). In this study two markers (RAPD and ISSR) based on DNA amplification reactions were used to assess the genetic diversity of ecotypes and cultivars of P. pratensis.…”

Section: Discussionmentioning

confidence: 89%

Peer Review #1 of "Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers (v0.1)"

2016

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Background. Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation. Methods. The molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA). Results. The low value of Dice's coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin. Discussion. The populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.

show abstract

Comparison of Agronomic Characters, Total Seed Storage Proteins and their use for Genotypes Discrimination in the Kentucky Bluegrass (Poa PratensisL.)

Cited by 6 publications

References 9 publications

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Characterization of morphological traits and RAPD polymorphism in selected forms of Kentucky bluegrass (Poa pratensis L.)

Peer Review #1 of "Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers (v0.1)"

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