“…Most of the literature reports describe the purification of enzyme through selective ammonium sulphate precipitation and chromatographic techniques like gel-filtration chromatography (Goyal and Anjan, 2008), ion-exchange chromatography based on CM (carboxymethyl) Sephadex, DEAE (diethylaminoethyl) Sephadex or DEAE cellulose (Aydemir and Kuru, 2003;Bilal et al, 2006). River buffalo (Bubalus bubalis) is a species of tremendous important in livestock and rural economy in Pakistan where it contributes 27 million tons of milk and 0.68 million tons of meat annually (Li and Schellhorn, 2007;Khan and Iqbal, 2009). Despite of having a great importance in agriculture and livestock, the river buffalo remained unexplored for its proteins, enzymes and related genes.…”