Purification and Properties of Liver Catalase from Water Buffalo (Bubalus bubalis)

Nadeem, Muhammad; Khan, Jalaluddin A.; Murtaza, Bibi Nazia; Muhammad, Khushi; Rauf, Abdul

doi:10.14737/journal.sajls/2015/3.2.51.55

Cited by 8 publications

(4 citation statements)

References 26 publications

(33 reference statements)

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“…Their peroxidatic functions overlap with GPx and catalase, and their catalytic efficiency is lower (~10 5 M −1 s −1 ) compared to GPx (~10 8 M −1 s −1 ) and catalase (~10 6 M −1 s −1 ) [ 179 ]. Furthermore, comparing Prdx Km for H 2 O 2 with that of GPx and catalase exceeding even the millimolar range [ 180 ] suggests that the role of Prdx is rather as a sensor of H 2 O 2 [ 178 ] than oxidative stress condition reversal.…”

Section: Peroxiredoxins (Prdx)mentioning

confidence: 99%

Glutathione-Related Enzymes and Proteins: A Review

Vašková

Kočan²,

Vaško

et al. 2023

Molecules

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The tripeptide glutathione is found in all eukaryotic cells, and due to the compartmentalization of biochemical processes, its synthesis takes place exclusively in the cytosol. At the same time, its functions depend on its transport to/from organelles and interorgan transport, in which the liver plays a central role. Glutathione is determined as a marker of the redox state in many diseases, aging processes, and cell death resulting from its properties and reactivity. It also uses other enzymes and proteins, which enables it to engage and regulate various cell functions. This paper approximates the role of these systems in redox and detoxification reactions such as conjugation reactions of glutathione-S-transferases, glyoxylases, reduction of peroxides through thiol peroxidases (glutathione peroxidases, peroxiredoxins) and thiol–disulfide exchange reactions catalyzed by glutaredoxins.

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Section: Peroxiredoxins (Prdx)mentioning

confidence: 99%

Glutathione-Related Enzymes and Proteins: A Review

Vašková

Kočan²,

Vaško

et al. 2023

Molecules

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“…The optimal pH and temperature in the Purification and Properties of Liver Catalase in Water Buffalo (Bubalus bubalis) investigation were determined to be 7.5 and 30 C, respectively (Nadeem et al, 2015).…”

Section: Table 2 Activity Measurements Of Aronia Fruit Leaf Tissue Ca...mentioning

confidence: 99%

Characterization of Catalase Enzyme from Leaf Tissue of Aronia (Aronia melanocarpa) Plant

Taş

MİTROVİCA

Ekinci

2023

ANAJAS

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Aronia is among the most antioxidant containing plants which is found commonly around the world. Aronia cultivation started in Turkey for the first time in 2012 at the Atatürk Central Research Institute of Garden Cultures, and a plantation was constructed in the experimental area. Since antioxidants help to preserve food by blocking oxidation processes and contributing to the health promotion provided by numerous dietary supplements, nutraceutical and functional food additives, antioxidant capacity of these plants should be well characterized. To assess and evaluate the antioxidant content of foods and plant products, many approaches are utilized. In this study, catalase (CAT) enzyme was partially purified from aronia plant leaf tissue and characterization was carried out. Purification process consisted of homogenate preparation, ammonium sulfate precipitation and dialysis. The optimal ionic strength, pH, substrate concentration and enzyme quantity were examined. These values were found to be 300 mM Tris, pH:8.0, 12 mM H2O2 and 75 μl, respectively, for the catalase enzyme of the Aronia plant leaf tissue. This study is the first in the literature dealing with the characterization of antioxidant enzyme from Aronia plant.

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“…Полученные осадки разрушали осмотическим шоком (в 50 мМ трис-HCl буфере, рН 7,5). Перекрёстное загрязнение определяли по активности маркерных ферментов цитоплазмы (алкогольдегидрогеназа [11]), митохондрий (сукцинатдегидрогеназа [12]) и пероксисом (каталаза [13]).…”

Section: методикаunclassified

Effect of jerusalem artichoke extract on the functioning of malate dehydrogenase in the liver of rats with alloxan diabetes

2021

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An increase in the activity and the appearance of a new isoform of NAD-dependent malate dehydrogenase (MDH; EC 1.1.1.37) has been detected in the liver of rats with alloxan diabetes was revealed. This confirms the possibility of MDH involvement in the adaptive reaction of the body under oxidative tress caused by biochemical changes in diabetic animals. The increase in the hepatic MDH activity in rats with experimental type I diabetes mellitus (T1DM) is associated with the formation of an additional MDH isoform in peroxisomes. Data on the expression of the MDH encoding genes mdh1 and mdh2 confirm that in T1DM the increase in MDH activity occurs at the level of transcription of MDH encoding genes. The use of the extract of Helianthus tuberosus led to a marked decrease in the blood glucose concentration of rats with alloxan diabetes, abolished by the change in transcriptional activity of the studied genes and blocked the formation of new MDH isoforms in rats with experimental alloxan diabetes. This suggest that extract of H. tuberosus may be of considerable interest from the point of view of pharmacological correction of metabolic changes during the development of pathologies of this kind.

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Purification and Properties of Liver Catalase from Water Buffalo (Bubalus bubalis)

Cited by 8 publications

References 26 publications

Glutathione-Related Enzymes and Proteins: A Review

Glutathione-Related Enzymes and Proteins: A Review

Characterization of Catalase Enzyme from Leaf Tissue of Aronia (Aronia melanocarpa) Plant

Effect of jerusalem artichoke extract on the functioning of malate dehydrogenase in the liver of rats with alloxan diabetes

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