Studies on the Chemistry of the Lichen Genus Umbilicaria Hoffm.

Posner, Birgit; Feige, G. B.; Huneck, Siegfried

doi:10.1515/znc-1992-1-202

Cited by 23 publications

(29 citation statements)

References 5 publications

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“…U. hirsuta contains GA as a main metabolite accompanied only by small amounts of lecanoric acid [23]. By using and novel, simple and economic procedure for extracting and purifying GA, we achieved a purity of 98, 2%.…”

Section: Discussionmentioning

confidence: 95%

Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Goga

Kello

Vilková

et al. 2019

BMC Complement Altern Med

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Background: Lichens produce a huge diversity of bioactive compounds with several biological effects. Gyrophoric acid (GA) is found in high concentrations in the common lichen Umbilicaria hirsuta, however evidence for biological activity was limited to anti-proliferative activity described on several cancer cell lines. Methods: We developed and validated a new protocol for GA isolation, resulting in a high yield of highly pure GA (validated by HPLC and NMR) in an easy and time saving manner. Anti-proliferative and pro-apoptotic activity, oxygen radicals formation and stress/survival proteins activity changes was study by flow cytometry. Results: The highly purified GA showed anti-proliferative activity against HeLa (human cervix carcinoma) and other tumor cells. Moreover, GA threated cells showed a significant increase in caspase-3 activation followed by PARP cleavage, PS externalization and cell cycle changes mediated by oxidative stress. Production of oxygen radicals led to DNA damage and changes in stress/survival pathways activation. Conclusions: GA treatment on HeLa cells clearly indicates ROS production and apoptosis as form of occurred cell death. Moreover, DNA damage and changing activity of stress/survival proteins as p38MAPK, Erk1/2 and Akt mediated by GA treatment confirm pro-apoptotic potential. The pharmacological potential of U. hirsuta derived GA is discussed.

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Section: Discussionmentioning

confidence: 95%

Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Goga

Kello

Vilková

et al. 2019

BMC Complement Altern Med

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“…Umbilicaria spodochroa contained gyrophoric acid (major) and atranorin (minor). Two minor unidentified compounds with spectra resembling gyrophoric acid may be lecanoric acid and hiasic acid, which can be expected in all characterized chemical races of the species (Posner et al 1992).…”

Section: Lichen Compoundsmentioning

confidence: 99%

Lichen Compounds Restrain Lichen Feeding by Bank Voles (Myodes glareolus)

et al. 2010

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Some lichen compounds are known to deter feeding by invertebrate herbivores. We attempted to quantify the deterring efficiency of lichen compounds against a generalist vertebrate, the bank vole (Myodes glareolus). In two separate experiments, caged bank voles had the choice to feed on lichens with natural or reduced concentrations of secondary compounds. We rinsed air-dry intact lichens in 100% acetone to remove extracellular compounds non-destructively. In the first experiment, pairs of control and rinsed lichen thalli were hydrated and offered to the bank voles. Because the lichens desiccated fast, we ran a second experiment with pairs of ground control and compound-deficient thalli, each mixed with water to porridge. Eight and six lichen species were tested in the first and second experiment, respectively. In the first, bank voles preferred compound-deficient thalli of Cladonia stellaris and Lobaria pulmonaria, but did not discriminate between the other thallus pairs. This was likely a result of deterring levels of usnic and stictic acid in the control thalli. When lichens were served as porridge, significant preference was found for acetone-rinsed pieces of Cladonia arbuscula, C. rangiferina, Platismatia glauca, and Evernia prunastri. The increased preference was caused mainly by lower consumption of control thalli. Grinding and mixing of thallus structures prevented bank voles from selecting thallus parts with lower concentration of secondary compounds and/or strengthened their deterring capacity. We conclude that some lichen secondary compounds deter feeding by bank voles.

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“…It can occur in major or minor quantities, or in traces within the same species, for example in Umbilicaria crustulosa (Ach.) Lamy (Posner et al 1992;Huneck et al 1993;Seriña et al 1996).…”

Section: Diagnostic Traits and Variabilitymentioning

confidence: 99%

Umbilicaria orientalis– a new species ofUmbilicariasubg.Papillophorawith an East Asian distribution: morphological delimitation and molecular evidence

et al. 2020

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Umbilicaria orientalis Davydov sp. nov. is described and phylogenetic analysis (ITS, mtLSU and RPB2) confirmed its distinctness and indicated a sister relationship with U. trabeculata within the U. vellea group. The species is morphologically similar to U. vellea but differs by simple, cylindrical or strap-like rhizinomorphs and by thalloconidia, developing both on the lower surface and on rhizinomorphs, that are 1–2 (rarely 4)–celled or in aggregates of up to 5–6 cells. Umbilicaria orientalis is described from the Russian Far East and is currently known from a wide range of localities in East Asia, from the Far East to South Siberia and Mongolia in the north, to Hebei and Tibet in the south. New sequences of U. americana were obtained; the species represents an independent phylogenetic lineage within Umbilicaria subg. Papillophora. Diagnostic traits and variability of different developmental stages of U. orientalis, as well as its East Asian distribution pattern, are discussed.

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Studies on the Chemistry of the Lichen Genus Umbilicaria Hoffm.

Cited by 23 publications

References 5 publications

Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Lichen Compounds Restrain Lichen Feeding by Bank Voles (Myodes glareolus)

Umbilicaria orientalis– a new species ofUmbilicariasubg.Papillophorawith an East Asian distribution: morphological delimitation and molecular evidence

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