Studies on the Binding of Barbaloin to Human Gamma Globulin

Lei, Ruixia; Hu, Zhide; Chen, Xingguo; Shen, Fenling; Jing, Jing

doi:10.1080/00387010600636999

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…All the domains of IgG have two cysteine (Cys) residues which form the intradomain disulfide bond and at least one tryptophan (Trp) residue which can protect against the hydrolysis by proteinase. 13,14) Studies of X-ray analysis of crystal structure on the light-chain dimer Mcgl have proved that V L has a constant Trp-37 residue and C L has a constant Trp-152 residue, and both of Trp residues are adjacent to disulfide bonds. Studies of X-ray analysis of crystal structure on the interaction of IgG1FabЈ (New) with VitK 1 OH have proved that the end of VitK 1 OH is adjacent to the Trp-54 of CDRs of IgG1FabЈ (New).…”

mentioning

confidence: 99%

“…The most binding sites of IgG for antigen are located in complement-determining region (CDR) of antigen-binding fragments (Fab), and the crystallizable fragments (Fc) can be considered to a certain extent as a similarity to Fab region and can also bind to smaller molecules. 13,14) Additionally, the framework region (FR), which consists of FR1, FR2, FR3 and FR4, provides a supporting framework for CDR of Fab of IgG. Recently studies have revealed that FR3 adjacent to binding site for antigen can also bind to antigens directly.…”

mentioning

confidence: 99%

“…10,11) In addition, IgG presents itself in the blood of adults at 9.5-12.5 mg/ml, and as one of human plasma proteins, it is capable of binding an extraordinarily diverse range of metabolites, drugs, organic compounds and relevant antigens. [12][13][14] With the remarkable binding properties, IVIG can serve as an important transport protein for drugs, which may have important roles in the discovery of novel drug delivery system and targeted drug therapy. By using its high-safety profile, clinicians may construct new protocols for patients.…”

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confidence: 99%

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Study on the Interactions of Kaempferol and Quercetin with Intravenous Immunoglobulin by Fluorescence Quenching, Fourier Transformation Infrared Spectroscopy and Circular Dichroism Spectroscopy

Zhou

Yao

et al. 2008

CHEMICAL & PHARMACEUTICAL BULLETIN

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Initially used as replacement therapy for patients with primary and secondary immune deficiencies, intravenous immunoglobulin (IVIG), the human serum immunoglobulin (Ig) fraction that is mainly composed of normal human polyclonal IgG obtained from plasma pools from a large number of healthy blood donors, is now widely used for the treatment of a large number of autoimmune and systemic inflammatory diseases. 1,2) Recently, great attention has been paid to IVIG potential use as adjuvant anti-neoplastic agent.3-5) Studies on the anticancer alkaloid vindesine (VDS) conjugates of the anti-CEA (carcinoembryonic antigen) antibodies against a colorectal tumor xenograft revealed that antibody 14.95.55 (IgG2a) suppressed tumor growth both alone and as a VDS conjugate, while 11.285.14 (IgG1) produced only a slight effect alone but an almost complete and lasting suppression of tumor growth as a VDS conjugate. Acute studies showed that VDS-11.285.14 conjugate was considerably less toxic than free VDS in Balb/c mice.6) It has been revealed that there are different transfer mechanism between the drug-protein conjugates and free drugs transferred into cells, and the conjugates can reduce the toxicity of drugs, resulting in a prolongation of survival time.7) Studies on the efficacy of an IgG F(ab) 2 preparation against taxol-induced C activation revealed that at a therapeutically relevant dose level (10 mg/ml), this Fc-depleted IgG caused significant suppression of taxol-induced SC5b-9 formation, but replacing IVIG with 10 mg/ml human serum albumin (HSA) caused no inhibition of taxolinduced rise of SC5b-9. 8) Also, it is reported that within a range of molar concentration ratio of taxol to IVIG, the interaction of IVIG with taxol can inhibit taxol from crystallizing in aqueous solution.9) Studies in vitro have revealed that IVIG may stimulate the production of IL-12, an anti-tumor and anti-angiogenic cytokine, and enhanced NK cell activity.4) Studies also revealed that IVIG can decrease the level of matrix metalloproteinase-9 (MMP-9) expression in the U937 monocyte line with a decrease in the m-RNA level of MMP-9 that participates in basement membrane degradation, a vital step in the invasion of metastatic cancer cells. So, IVIG may serve as an adjuvant therapy in cancer due to its anti-neoplastic properties that may synergize with more specific MMP inhibitors and other anti-cancer drugs. 10,11) In addition, IgG presents itself in the blood of adults at 9.5-12.5 mg/ml, and as one of human plasma proteins, it is capable of binding an extraordinarily diverse range of metabolites, drugs, organic compounds and relevant antigens. [12][13][14] With the remarkable binding properties, IVIG can serve as an important transport protein for drugs, which may have important roles in the discovery of novel drug delivery system and targeted drug therapy. By using its high-safety profile, clinicians may construct new protocols for patients.Flavonols are plant pigments that are ubiquitous in nature. Kaempferol (3,4Ј,5,7-pentahydroxyflavone), quer...

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Study on the Interactions of Kaempferol and Quercetin with Intravenous Immunoglobulin by Fluorescence Quenching, Fourier Transformation Infrared Spectroscopy and Circular Dichroism Spectroscopy

Zhou

Yao

et al. 2008

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Although most of drug-protein binding involves non-covalent hydrophobic forces, they are adequate to form a very tight bond to specifi c sites of some proteins (22). Temperature and time are among the main factors that aff ect the degree of wogonin protein binding, as previously reported (23)(24)(25). These reports showed that the binding process was primarily exothermic, spontaneous, enthalpy and entropy driven.…”

Section: Wogonin Protein Binding and Plasma Distributionmentioning

confidence: 56%

“…Chromatographic separation was performed on a Hedera ODS-2 column (4.6 mm in diameter and 250 mm length, 5 μm). Isocratic mobile phase was used in this method, as previously reported (16,(22)(23)(24). It is composed of 75 % of methanol and 25 % of ammonium acetate buff er (77 mg L -1 , pH adjusted to 4 with acetic acid).…”

Section: Uv-vis Spectrophotometric Analysis and Hplc Conditionsmentioning

confidence: 99%

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Salmani

Jacob

et al. 2017

Acta Pharmaceutica

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In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experiencein vivowhile pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

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Interaction of intravenous immunoglobulin and three 20(S)-camptothecin analogs: maintaining higher circulatory levels of the biologically active species

Li¹,

Yao

et al. 2017

Med Chem Res

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Studies on the Binding of Barbaloin to Human Gamma Globulin

Cited by 10 publications

References 22 publications

Study on the Interactions of Kaempferol and Quercetin with Intravenous Immunoglobulin by Fluorescence Quenching, Fourier Transformation Infrared Spectroscopy and Circular Dichroism Spectroscopy

Study on the Interactions of Kaempferol and Quercetin with Intravenous Immunoglobulin by Fluorescence Quenching, Fourier Transformation Infrared Spectroscopy and Circular Dichroism Spectroscopy

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Interaction of intravenous immunoglobulin and three 20(S)-camptothecin analogs: maintaining higher circulatory levels of the biologically active species

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